Abstract
Indigenous rhizobia were isolated from root nodules of lentil plants collected from various agro-climatic regions of India. These isolates together with four standard lentil Rhizobium strains were screened for sensitivity against eight phages. Four strains, USDA 2431, BHULR 104, BHULR 113 and BHULR 115 having restricted sensitivity to lytic phages LRP-1, LRP-4, LRP-13 and LRP-15 respectively, were characterized for both physiological and molecular characters. All strains had a generation time of between 3.8 and 5.6 h in yeast extract–mannitol (YM) broth, and colonies on YM agar plates showed an acidic reaction. Compared to other strains, strain USDA 2431 grew poorly when sucrose was the sole carbon source and showed maximum growth in arabinose-containing medium. The intrinsic antibiotic resistance level in all strains was tested against seven antibiotics and found to be high with ampicillin and kanamycin (50–60 μg ml -1 ) but very low with neomycin (0.03 μg ml -1 ). With the exception of strain BHULR 113, all strains expressed ex planta nitrogenase activity, with strain USDA 2431 showing the maximum activity (26.8 nmol C 2 H 4 h -1 mg -1 protein) after 6 h of incubation. Genomic and phylogenetic relationships among the strains were examined by randomly amplified polymorphic DNA and 16S rRNA sequence analysis. Genetic distance varied from 0.09 to 0.23 among the strains, and the primer OPL-11 was found to be suitable for the discrimination of these strains. The 16S rRNA sequence analysis revealed 99–100% similarity with Rhizobium leguminosarum bv. viciae. These results clearly indicate that phage sensitivity is a useful marker for discriminating locally soil-adapted rhizobial strains forming effective nodules in lentil.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 10
Claim · model-written summary
Strain USDA 2431 showed the maximum ex planta nitrogenase activity of 26.8 nmol C2H4 h-1 mg-1 protein after 6 h of incubation.
- Taxon
- lentil
Lens culinarist0 - Material
- USDA 2431e4
- Outcome
- ex planta nitrogenase activitye4
- Value
- 26.8 nmol C 2 H 4 h -1 mg -1 proteine4
- Qualifier
- after 6 h of incubatione4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Physiological and molecular characterization of locally adapted Rhizobium strains of lentil (Lens culinaris Medik.) having restricted phage sensitivity”
e4“With the exception of strain BHULR 113, all strains expressed ex planta nitrogenase activity, with strain USDA 2431 showing the maximum activity (26.8 nmol C 2 H 4 h -1 mg -1 protein) after 6 h of incubation”
Claim · model-written summary
All Rhizobium strains of lentil except BHULR 113 expressed ex planta nitrogenase activity.
- Taxon
- lentilLens culinarist0
- Outcome
- ex planta nitrogenase activitye4
- Qualifier
- With the exception of strain BHULR 113e4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Physiological and molecular characterization of locally adapted Rhizobium strains of lentil (Lens culinaris Medik.) having restricted phage sensitivity”
e4“With the exception of strain BHULR 113, all strains expressed ex planta nitrogenase activity, with strain USDA 2431 showing the maximum activity (26.8 nmol C 2 H 4 h -1 mg -1 protein) after 6 h of incubation”
Claim · model-written summary
Strain USDA 2431 grew poorly when sucrose was the sole carbon source compared with arabinose-containing medium.
- Taxon
- lentilLens culinarist0
- Material
- USDA 2431e3
- Treatment
- sucrose was the sole carbon sourcee3
- Outcome
- growthe3
- Direction
- poorlye3
- Comparator
- arabinose-containing mediume3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Physiological and molecular characterization of locally adapted Rhizobium strains of lentil (Lens culinaris Medik.) having restricted phage sensitivity”
e3“strain USDA 2431 grew poorly when sucrose was the sole carbon source and showed maximum growth in arabinose-containing medium”
Claim · model-written summary
Strain USDA 2431 showed maximum growth in arabinose-containing medium compared with sucrose as sole carbon source.
- Taxon
- lentilLens culinarist0
- Material
- USDA 2431e3
- Treatment
- arabinose-containing mediume3
- Outcome
- growthe3
- Direction
- maximume3
- Comparator
- sucrose was the sole carbon sourcee3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Physiological and molecular characterization of locally adapted Rhizobium strains of lentil (Lens culinaris Medik.) having restricted phage sensitivity”
e3“strain USDA 2431 grew poorly when sucrose was the sole carbon source and showed maximum growth in arabinose-containing medium”
Claim · model-written summary
All strains of lentil Rhizobium had a generation time of 3.8 to 5.6 h in YM broth.
- Taxon
- lentilLens culinarist0
- Material
- All strainse2
- Outcome
- generation timee2
- Value
- between 3.8 and 5.6 he2
- Setting
- yeast extract–mannitol (YM) brothe2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Physiological and molecular characterization of locally adapted Rhizobium strains of lentil (Lens culinaris Medik.) having restricted phage sensitivity”
e2“All strains had a generation time of between 3.8 and 5.6 h in yeast extract–mannitol (YM) broth”
Claim · model-written summary
Strains USDA 2431, BHULR 104, BHULR 113 and BHULR 115 of lentil Rhizobium had restricted sensitivity to lytic phages.
- Taxon
- lentilLens culinarist0
- Material
- USDA 2431, BHULR 104, BHULR 113 and BHULR 115e1
- Outcome
- sensitivity to lytic phagese1
- Qualifier
- restrictede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Physiological and molecular characterization of locally adapted Rhizobium strains of lentil (Lens culinaris Medik.) having restricted phage sensitivity”
e1“Four strains, USDA 2431, BHULR 104, BHULR 113 and BHULR 115 having restricted sensitivity to lytic phages LRP-1, LRP-4, LRP-13 and LRP-15 respectively”
Claim · model-written summary
Genetic distance among the lentil Rhizobium strains varied from 0.09 to 0.23.
- Taxon
- lentilLens culinarist0
- Outcome
- Genetic distancee5
- Value
- 0.09 to 0.23e5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Physiological and molecular characterization of locally adapted Rhizobium strains of lentil (Lens culinaris Medik.) having restricted phage sensitivity”
e5“Genetic distance varied from 0.09 to 0.23 among the strains”
Claim · model-written summary
The 16S rRNA sequences of the lentil Rhizobium strains showed 99–100% similarity with Rhizobium leguminosarum bv. viciae.
- Taxon
- lentilLens culinarist0
- Outcome
- similarity with Rhizobium leguminosarum bv. viciaee6
- Value
- 99–100%e6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Physiological and molecular characterization of locally adapted Rhizobium strains of lentil (Lens culinaris Medik.) having restricted phage sensitivity”
e6“The 16S rRNA sequence analysis revealed 99–100% similarity with Rhizobium leguminosarum bv. viciae”
Claim · model-written summary
Primer OPL-11 was suitable for discriminating the lentil Rhizobium strains.
- Taxon
- lentilLens culinarist0
- Outcome
- discrimination of these strainse7
- Qualifier
- the primer OPL-11 was found to be suitablee7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Physiological and molecular characterization of locally adapted Rhizobium strains of lentil (Lens culinaris Medik.) having restricted phage sensitivity”
e7“the primer OPL-11 was found to be suitable for the discrimination of these strains”
Claim · model-written summary
Phage sensitivity is a useful marker for discriminating locally soil-adapted rhizobial strains of lentil.
- Taxon
- lentilLens culinarise1
- Outcome
- phage sensitivity is a useful markere1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Physiological and molecular characterization of locally adapted Rhizobium strains of lentil (Lens culinaris Medik.) having restricted phage sensitivity”
e1“These results clearly indicate that phage sensitivity is a useful marker for discriminating locally soil-adapted rhizobial strains forming effective nodules in lentil”
The same questions in other papers
in vitro · growth · Lens culinaris (lentil)Results agree
4 papers in the same direction as this paper
- positive
- positive
- positive
1 paper more in this group
See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.