Abstract
A test was carried out to see if sucrose could regulate cold-acclimation-associated gene expression in Arabidopsis. In plants and excised leaves, sucrose caused an increase in GUS activity, as a reporter for the activity of the cold-responsive COR78 promoter. This increase was transient at 21 degrees C but lasted for at least 4 d at 4 degrees C in continuous darkness. However, at 4 degrees C with a 16 h photoperiod, GUS activity was similarly high with solutions lacking sucrose or with different concentrations of sucrose. In peeled lower epidermis in the cold dark environment, 40 mM sucrose increased COR78 transcript abundance to substantially above that in the controls, but sorbitol had no effect. Similarly to the cold and dark conditions, sucrose increased COR78 transcript abundance in the epidermis in the warm light and warm dark environments, but not in a cold light environment. Sucrose had much less effect on COR78 transcript abundance in leaves without the lower epidermis. Thus sucrose regulates expression of COR78, possibly mainly in the epidermis, at the level of transcription. Furthermore, 40 mM sucrose at 4 degrees C for 24 h in constant darkness was sufficient to give the same GUS activity as in fully acclimated plants of the same age in a 16 h photoperiod, although by 48 h, GUS activity had become intermediate between control and fully cold-acclimated plants. Thus sucrose has a regulatory role in the acclimation of whole plants to cold and this may be important during diurnal dark periods.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by-nc. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 14
Claim · model-written summary
Sucrose increased COR78 promoter reporter GUS activity in plants and excised leaves.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianat0 - Organ / stage
- leavese0
- Treatment
- sucrosee0
- Gene
- COR78e0
- Outcome
- GUS activitye0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In plants and excised leaves, sucrose caused an increase in GUS activity, as a reporter for the activity of the cold-responsive COR78 promoter.”
t0“Arabidopsis thaliana”
Claim · model-written summary
At 21 degrees C the sucrose induced GUS increase was transient.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Outcome
- increasee0
- Qualifier
- transient at 21 degrees Ce0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This increase was transient at 21 degrees C”
t0“Arabidopsis thaliana”
Claim · model-written summary
At 4 degrees C in continuous darkness the GUS increase lasted at least four days.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Outcome
- GUS activitye1
- Value
- at least 4 de0
- Qualifier
- at 4 degrees C in continuous darknesse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“lasted for at least 4 d at 4 degrees C in continuous darkness.”
t0“Arabidopsis thaliana”
e1“In plants and excised leaves, sucrose caused an increase in GUS activity, as a reporter for the activity of the cold-responsive COR78 promoter.”
Claim · model-written summary
Under cold photoperiod conditions GUS activity was similarly high with and without sucrose.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- different concentrations of sucrosee0
- Outcome
- GUS activitye0
- Direction
- similarly highe0
- Qualifier
- at 4 degrees C with a 16 h photoperiode0
- Comparator
- solutions lacking sucrosee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“at 4 degrees C with a 16 h photoperiod, GUS activity was similarly high with solutions lacking sucrose or with different concentrations of sucrose.”
t0“Arabidopsis thaliana”
Claim · model-written summary
In cold dark peeled epidermis, 40 mM sucrose increased COR78 transcripts above controls.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Organ / stage
- peeled lower epidermise0
- Treatment
- 40 mM sucrosee0
- Gene
- COR78e0
- Outcome
- COR78 transcript abundancee0
- Direction
- increasede0
- Qualifier
- cold dark environmente0
- Comparator
- controlse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In peeled lower epidermis in the cold dark environment, 40 mM sucrose increased COR78 transcript abundance to substantially above that in the controls”
t0“Arabidopsis thaliana”
Claim · model-written summary
Sorbitol did not affect COR78 transcript abundance in cold dark epidermis.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- sorbitole0
- Outcome
- COR78 transcript abundancee1
- Direction
- no effecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“but sorbitol had no effect.”
t0“Arabidopsis thaliana”
e1“In peeled lower epidermis in the cold dark environment, 40 mM sucrose increased COR78 transcript abundance to substantially above that in the controls, but sorbitol had no effect.”
Claim · model-written summary
Sucrose increased epidermal COR78 transcript abundance in warm light.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Organ / stage
- epidermise0
- Treatment
- sucrosee0
- Gene
- COR78e0
- Outcome
- COR78 transcript abundancee0
- Direction
- increasede0
- Qualifier
- warm lighte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“sucrose increased COR78 transcript abundance in the epidermis in the warm light and warm dark environments”
t0“Arabidopsis thaliana”
Claim · model-written summary
Sucrose increased epidermal COR78 transcript abundance in warm darkness.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Organ / stage
- epidermise0
- Treatment
- sucrosee0
- Gene
- COR78e0
- Outcome
- COR78 transcript abundancee0
- Direction
- increasede0
- Qualifier
- warm darke0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“sucrose increased COR78 transcript abundance in the epidermis in the warm light and warm dark environments”
t0“Arabidopsis thaliana”
Claim · model-written summary
Sucrose did not increase epidermal COR78 transcript abundance in cold light.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- sucrosee1
- Outcome
- COR78 transcript abundancee2
- Direction
- note0
- Qualifier
- cold light environmente0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“but not in a cold light environment.”
t0“Arabidopsis thaliana”
e1“Similarly to the cold and dark conditions, sucrose increased COR78 transcript abundance in the epidermis in the warm light and warm dark environments, but not in a cold light environment.”
e2“In peeled lower epidermis in the cold dark environment, 40 mM sucrose increased COR78 transcript abundance to substantially above that in the controls, but sorbitol had no effect.”
Claim · model-written summary
Sucrose had much less effect on COR78 transcripts in leaves without lower epidermis.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Organ / stage
- leaves without the lower epidermise0
- Treatment
- Sucrosee0
- Gene
- COR78e0
- Outcome
- COR78 transcript abundancee0
- Direction
- much lesse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Sucrose had much less effect on COR78 transcript abundance in leaves without the lower epidermis.”
t0“Arabidopsis thaliana”
Claim · model-written summary
Forty mM sucrose for 24 h under cold darkness yielded GUS activity equal to fully acclimated plants.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- 40 mM sucrose at 4 degrees C for 24 h in constant darknesse0
- Outcome
- GUS activitye0
- Direction
- samee0
- Comparator
- fully acclimated plants of the same age in a 16 h photoperiode0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“40 mM sucrose at 4 degrees C for 24 h in constant darkness was sufficient to give the same GUS activity as in fully acclimated plants of the same age in a 16 h photoperiod”
t0“Arabidopsis thaliana”
Claim · model-written summary
By 48 h GUS activity was intermediate between control and fully acclimated plants.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Outcome
- GUS activitye0
- Direction
- intermediatee0
- Qualifier
- by 48 he0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“by 48 h, GUS activity had become intermediate between control and fully cold-acclimated plants.”
t0“Arabidopsis thaliana”
Claim · model-written summary
Authors infer that sucrose regulates COR78 expression mainly in epidermis at transcription.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Gene
- COR78e0
- Outcome
- expression of COR78e0
- Qualifier
- at the level of transcriptione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“sucrose regulates expression of COR78, possibly mainly in the epidermis, at the level of transcription.”
t0“Arabidopsis thaliana”
Claim · model-written summary
Authors infer a regulatory role for sucrose in whole plant cold acclimation.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Outcome
- acclimation of whole plants to colde0
- Qualifier
- during diurnal dark periodse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“sucrose has a regulatory role in the acclimation of whole plants to cold and this may be important during diurnal dark periods.”
t0“Arabidopsis thaliana”
The same questions in other papers
glucitol · molecular: expression · Arabidopsis thaliana (thale cress)Positive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- positive
- no effect
sucrose · molecular: expression · Arabidopsis thaliana (thale cress)Positive and negative results
8 papers in the same direction as this paper · 5 in the opposite direction
- negative
- negative
- negative
10 papers more in this group
See all 14 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.