Abstract
Cultured cells of rose (Rosa damascena) treated with an elicitor derived from Phytophthora spp. and suspension-cultured cells of French bean (Phaseolus vulgaris) treated with an elicitor derived from the cell walls of Colletotrichum lindemuthianum both produced H2O2. It has been hypothesized that in rose cells H2O2 is produced by a plasma membrane NAD(P)H oxidase (superoxide synthase), whereas in bean cells H2O2 is derived directly from cell wall peroxidases following extracellular alkalinization and the appearance of a reductant. In the rose/Phytophthora spp. system treated with N, N-diethyldithiocarbamate, superoxide was detected by a N, N'-dimethyl-9,9'-biacridium dinitrate-dependent chemiluminescence; in contrast, in the bean/C. lindemuthianum system, no superoxide was detected, with or without N,N-diethyldithiocarbamate. When rose cells were washed free of medium (containing cell wall peroxidase) and then treated with Phytophthora spp. elicitor, they accumulated a higher maximum concentration of H2O2 than when treated without the washing procedure. In contrast, a washing treatment reduced the H2O2 accumulated by French bean cells treated with C. lindemuthianum elicitor. Rose cells produced reductant capable of stimulating horseradish (Armoracia lapathifolia) peroxidase to form H2O2 but did not have a peroxidase capable of forming H2O2 in the presence of reductant. Rose and French bean cells thus appear to be responding by different mechanisms to generate the oxidative burst.
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Claims 9
Claim · model-written summary
Rose cells treated with a Phytophthora elicitor produced H2O2.
- Taxon
- rosee0
- Treatment
- an elicitor derived from Phytophthora spp.e0
- Interactor
- Phytophthora spp.
Phytophthorae0pathogen - Outcome
- produced H2O2e0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cultured cells of rose (Rosa damascena) treated with an elicitor derived from Phytophthora spp. and suspension-cultured cells of French bean (Phaseolus vulgaris) treated with an elicitor derived from the cell walls of Colletotrichum lindemuthianum both produced H2O2.”
Claim · model-written summary
French bean cells treated with a Colletotrichum elicitor produced H2O2.
- Taxon
- French bean
Phaseolus vulgarise0 - Treatment
- an elicitor derived from the cell walls of Colletotrichum lindemuthianume0
- Interactor
- Colletotrichum lindemuthianum
Colletotrichum lindemuthianume0pathogen - Outcome
- produced H2O2e0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“suspension-cultured cells of French bean (Phaseolus vulgaris) treated with an elicitor derived from the cell walls of Colletotrichum lindemuthianum both produced H2O2.”
Claim · model-written summary
Superoxide was detected in elicited rose cells treated with N, N-diethyldithiocarbamate.
- Taxon
- rosee0
- Treatment
- N, N-diethyldithiocarbamatee0
- Interactor
- Phytophthora spp.Phytophthorae0pathogen
- Outcome
- superoxide was detectede0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In the rose/Phytophthora spp. system treated with N, N-diethyldithiocarbamate, superoxide was detected”
Claim · model-written summary
Superoxide was not detected in elicited bean cells with or without N,N-diethyldithiocarbamate.
- Taxon
- beane0
- Interactor
- C. lindemuthianumColletotrichum lindemuthianume0pathogen
- Outcome
- no superoxide was detectede0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in the bean/C. lindemuthianum system, no superoxide was detected, with or without N,N-diethyldithiocarbamate.”
Claim · model-written summary
Washing rose cells before Phytophthora elicitation increased their maximum H2O2 concentration.
- Taxon
- rosee0
- Treatment
- washed free of mediume0
- Interactor
- Phytophthora spp.Phytophthorae0pathogen
- Outcome
- maximum concentration of H2O2e0
- Direction
- highere0
- Comparator
- without the washing proceduree0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When rose cells were washed free of medium (containing cell wall peroxidase) and then treated with Phytophthora spp. elicitor, they accumulated a higher maximum concentration of H2O2 than when treated without the washing procedure.”
Claim · model-written summary
Washing reduced H2O2 accumulation in French bean cells given Colletotrichum elicitor.
- Taxon
- French beanPhaseolus vulgarise0
- Treatment
- washing treatmente0
- Interactor
- C. lindemuthianumColletotrichum lindemuthianume0pathogen
- Outcome
- H2O2 accumulatede0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a washing treatment reduced the H2O2 accumulated by French bean cells treated with C. lindemuthianum elicitor.”
Claim · model-written summary
Rose cells produced a reductant able to stimulate horseradish peroxidase to form H2O2.
Verbatim
e0“Rose cells produced reductant capable of stimulating horseradish (Armoracia lapathifolia) peroxidase to form H2O2”
Claim · model-written summary
Rose cells lacked a peroxidase able to form H2O2 with reductant.
Verbatim
e0“did not have a peroxidase capable of forming H2O2 in the presence of reductant.”
e1“Rose cells produced reductant”
Claim · model-written summary
Rose and French bean cells appear to generate oxidative bursts through different mechanisms.
Verbatim
e0“Rose and French bean cells thus appear to be responding by different mechanisms to generate the oxidative burst.”
The same questions in other papers
processing · Colletotrichum lindemuthianum · Phaseolus vulgaris (common bean)Positive and negative results
1 in the opposite direction
- positive
resistance inducer · physiology: oxidative · Phaseolus vulgaris (common bean)Results agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.