Abstract
Inward-rectifying potassium (K+(in)) channels in guard cells have been suggested to provide a pathway for K+ uptake into guard cells during stomatal opening. To test the proposed role of guard cell K+(in) channels in light-induced stomatal opening, transgenic Arabidopsis plants were generated that expressed dominant negative point mutations in the K+(in) channel subunit KAT1. Patch-clamp analyses with transgenic guard cells from independent lines showed that K+(in) current magnitudes were reduced by approximately 75% compared with vector-transformed controls at -180 mV, which resulted in reduction in light-induced stomatal opening by 38% to 45% compared with vector-transformed controls. Analyses of intracellular K+ content using both sodium hexanitrocobaltate (III) and elemental x-ray microanalyses showed that light-induced K+ uptake was also significantly reduced in guard cells of K+(in) channel depressor lines. These findings support the model that K+(in) channels contribute to K+ uptake during light-induced stomatal opening. Furthermore, transpirational water loss from leaves was reduced in the K+(in) channel depressor lines. Comparisons of guard cell K+(in) current magnitudes among four different transgenic lines with different K+(in) current magnitudes show the range of activities of K+(in) channels required for guard cell K+ uptake during light-induced stomatal opening.
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Claims 6
Claim · model-written summary
Dominant negative channel lines had approximately 75% lower inward K+ current than vector controls at −180 mV.
- Taxon
- Arabidopsis
Arabidopsist0 - Treatment
- Dominant Negative Guard Cell K+ Channel Mutantst1
- Outcome
- K+(in) current magnitudese0
- Direction
- reducede0
- Value
- approximately 75%e0
- Qualifier
- -180 mVe0
- Comparator
- vector-transformed controlse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“K+(in) current magnitudes were reduced by approximately 75% compared with vector-transformed controls at -180 mV”
t0“Arabidopsis”
t1“Dominant Negative Guard Cell K+ Channel Mutants”
Claim · model-written summary
Dominant negative channel lines had 38% to 45% less light-induced stomatal opening than vector controls.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- Dominant Negative Guard Cell K+ Channel Mutantst1
- Outcome
- light-induced stomatal openinge0
- Direction
- reductione0
- Value
- 38% to 45%e0
- Comparator
- vector-transformed controlse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“reduction in light-induced stomatal opening by 38% to 45% compared with vector-transformed controls”
t0“Arabidopsis”
t1“Dominant Negative Guard Cell K+ Channel Mutants”
Claim · model-written summary
Light-induced guard-cell K+ uptake was significantly reduced in channel depressor lines.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- K+(in) channel depressor linese0
- Outcome
- light-induced K+ uptakee0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“light-induced K+ uptake was also significantly reduced in guard cells of K+(in) channel depressor lines”
t0“Arabidopsis”
Claim · model-written summary
Transpirational water loss from leaves was reduced in channel depressor lines.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- K+(in) channel depressor linese0
- Outcome
- transpirational water loss from leavese0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transpirational water loss from leaves was reduced in the K+(in) channel depressor lines”
t0“Arabidopsis”
Claim · model-written summary
Differences among four transgenic lines delineated channel activities required for light-induced guard-cell K+ uptake.
- Taxon
- ArabidopsisArabidopsist0
- Material
- transgenic linese0
- Outcome
- range of activities of K+(in) channels required for guard cell K+ uptakee0
- Value
- foure0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Comparisons of guard cell K+(in) current magnitudes among four different transgenic lines with different K+(in) current magnitudes show the range of activities of K+(in) channels required for guard cell K+ uptake during light-induced stomatal opening”
t0“Arabidopsis”
Claim · model-written summary
The findings support a contribution of inward K+ channels to guard-cell uptake during stomatal opening.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- K+(in) channelse0
- Outcome
- K+ uptake during light-induced stomatal openinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These findings support the model that K+(in) channels contribute to K+ uptake during light-induced stomatal opening”
t0“Arabidopsis”
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