Abstract
The plasma membrane (PM)-localized Na+/H+ antiporter Salt Overly Sensitive1 (SOS1) is essential for plant salt tolerance through facilitating Na+ efflux; however, how SOS1 localization and protein accumulation is regulated in plants remains elusive. Here, we report that Sorting Nexin 1 (SNX1) is required for plant salt-stress tolerance through affecting endosomal trafficking of SOS1 in Arabidopsis (Arabidopsis thaliana). Disruption of SNX1 caused salt hypersensitivity with increased Na+ accumulation and decreased Na+ efflux in Arabidopsis when challenged with high salinity stress. SNX1 co-localized and interacted with SOS1 in endosomes, promoting its PM localization and protein stability in plants under saline conditions. SOS1 overexpression promoted salt tolerance in the wild-type, whereas such effect was greatly compromised in the snx1-2 mutant. Pharmaceutical results showed that SOS1 recycling from the cytosol to the PM was largely blocked while its vacuolar degradation was accelerated in the snx1-2 mutant. Furthermore, salt-induced SOS1 phosphorylation enhanced its interaction and co-localization with SNX1, which is required for SOS1 PM localization in plants. Our study elucidates that SNX1 facilitates SOS1 PM localization and protein accumulation through endosomal trafficking, thereby enhancing salt tolerance in plants.
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Claims 11
Claim · model-written summary
SNX1 disruption caused salt hypersensitivity in Arabidopsis.
- Taxon
- Arabidopsis
Arabidopsise0 - Treatment
- Disruption of SNX1e0
- Gene
- SNX1e0
- Outcome
- salt hypersensitivitye0
- Direction
- causede0
- Qualifier
- high salinity stresse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Disruption of SNX1 caused salt hypersensitivity with increased Na+ accumulation and decreased Na+ efflux in Arabidopsis when challenged with high salinity stress.”
Claim · model-written summary
SNX1 disruption increased sodium accumulation.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- Disruption of SNX1e0
- Gene
- SNX1e0
- Outcome
- Na+ accumulatione0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Disruption of SNX1 caused salt hypersensitivity with increased Na+ accumulation and decreased Na+ efflux in Arabidopsis when challenged with high salinity stress.”
Claim · model-written summary
SNX1 disruption decreased sodium efflux.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- Disruption of SNX1e0
- Gene
- SNX1e0
- Outcome
- Na+ effluxe0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Disruption of SNX1 caused salt hypersensitivity with increased Na+ accumulation and decreased Na+ efflux in Arabidopsis when challenged with high salinity stress.”
Claim · model-written summary
SNX1 colocalized and interacted with SOS1 in endosomes.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- SNX1e0
- Outcome
- co-localized and interacted with SOS1e0
- Setting
- endosomese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SNX1 co-localized and interacted with SOS1 in endosomes”
t0“Arabidopsis”
Claim · model-written summary
SNX1 promoted SOS1 plasma membrane localization and stability in saline plants.
- Taxon
- plantsViridiplantaee0
- Gene
- SOS1e0
- Outcome
- PM localization and protein stabilitye0
- Qualifier
- under saline conditionse0
Verbatim
e0“SNX1 co-localized and interacted with SOS1 in endosomes, promoting its PM localization and protein stability in plants under saline conditions.”
Claim · model-written summary
SOS1 overexpression promoted wild-type salt tolerance.
- Taxon
- ArabidopsisArabidopsist0
- Material
- wild-typee0
- Treatment
- SOS1 overexpressione0
- Gene
- SOS1e0
- Outcome
- salt tolerancee0
- Direction
- promotede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SOS1 overexpression promoted salt tolerance in the wild-type”
t0“Arabidopsis”
Claim · model-written summary
The salt-tolerance benefit of SOS1 overexpression was greatly compromised in snx1-2.
- Taxon
- ArabidopsisArabidopsist0
- Material
- snx1-2 mutante0
- Outcome
- effecte0
- Direction
- greatly compromisede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“whereas such effect was greatly compromised in the snx1-2 mutant.”
t0“Arabidopsis”
Claim · model-written summary
SOS1 recycling to the plasma membrane was blocked in snx1-2.
- Taxon
- ArabidopsisArabidopsist0
- Material
- snx1-2 mutante0
- Gene
- SOS1e0
- Outcome
- recycling from the cytosol to the PMe0
- Direction
- largely blockede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SOS1 recycling from the cytosol to the PM was largely blocked while its vacuolar degradation was accelerated in the snx1-2 mutant.”
t0“Arabidopsis”
Claim · model-written summary
SOS1 vacuolar degradation accelerated in snx1-2.
- Taxon
- ArabidopsisArabidopsist0
- Material
- snx1-2 mutante0
- Gene
- SOS1e0
- Outcome
- vacuolar degradatione0
- Direction
- acceleratede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SOS1 recycling from the cytosol to the PM was largely blocked while its vacuolar degradation was accelerated in the snx1-2 mutant.”
t0“Arabidopsis”
Claim · model-written summary
Salt-induced SOS1 phosphorylation enhanced interaction and colocalization with SNX1.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- salt-induced SOS1 phosphorylatione0
- Gene
- SOS1e0
- Outcome
- interaction and co-localization with SNX1e0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“salt-induced SOS1 phosphorylation enhanced its interaction and co-localization with SNX1”
t0“Arabidopsis”
Claim · model-written summary
SOS1 phosphorylation-dependent interaction with SNX1 was required for plasma membrane localization.
- Taxon
- plantsViridiplantaee0
- Gene
- SOS1e0
- Outcome
- PM localizatione0
- Qualifier
- requirede0
Verbatim
e0“which is required for SOS1 PM localization in plants.”
The same questions in other papers
loss of function · physiology: ion balance · ArabidopsisPositive and negative results
2 in the opposite direction
- negative
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.