Abstract
Numerous environmental factors influence isoflavone accumulation and have long hampered their genetic dissection. Temperature and water regimes are two of the most significant abiotic factors. However, while the effects of temperature have been widely studied, little is known about how water scarcity might affect isoflavone concentration in seeds. Studies have shown that accumulation of isoflavones is promoted by well-watered conditions, but the molecular basis remains elusive. The length and severity of the water stress required to induce changes are also still unknown. In the present work, several intensities of water stress were evaluated at various critical stages for soybean [Glycine max (L.) Merr.] seed development, in both field and controlled environments. The results suggested that only long-term progressive drought, spanning most of the seed developmental stages, significantly decreased isoflavone content in seeds. The reduction is proportional to the intensity of the stress and appears to occur in a genotype-dependent manner. However, regardless of water regime, isoflavone compounds were mainly accumulated in the later seed developmental stages. Transcripts of the most important genes for isoflavone biosynthesis were also quantified from samples collected at key seed developmental stages under well-watered and long-term water deficit conditions. Expression of CHS7, CHS8 and IFS2 correlated with isoflavone accumulation under well-watered conditions. Interestingly, we found that the two isoflavone synthase genes in soybean (IFS1 and IFS2) showed different patterns of expression. The abundance of IFS1 transcripts was maintained at a constant rate, whereas IFS2 was down-regulated and highly correlated with isoflavone accumulation under both water deficit and well-watered conditions, suggesting IFS2 as a main contributor to isoflavone diminution under drought.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 8
Claim · model-written summary
Long-term progressive drought significantly decreased soybean seed isoflavone content.
- Taxon
- soybean
Glycine maxe1 - Organ / stage
- seedse0
- Treatment
- long-term progressive droughte0
- Outcome
- isoflavone content in seedse0
- Direction
- decreasede0
- Qualifier
- spanning most of the seed developmental stagese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“only long-term progressive drought, spanning most of the seed developmental stages, significantly decreased isoflavone content in seeds”
e1“soybean [Glycine max (L.) Merr.] seed development”
Claim · model-written summary
Isoflavone reduction was proportional to drought intensity and genotype dependent.
- Taxon
- soybeanGlycine maxe1
- Treatment
- intensity of the stresse0
- Outcome
- The reductione0
- Direction
- proportionale0
- Qualifier
- genotype-dependent mannere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The reduction is proportional to the intensity of the stress and appears to occur in a genotype-dependent manner.”
e1“soybean [Glycine max (L.) Merr.] seed development”
Claim · model-written summary
Isoflavones accumulated mainly in later soybean seed developmental stages regardless of water regime.
- Taxon
- soybeanGlycine maxe1
- Organ / stage
- later seed developmental stagese0
- Outcome
- isoflavone compounds were mainly accumulatede0
- Qualifier
- regardless of water regimee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“regardless of water regime, isoflavone compounds were mainly accumulated in the later seed developmental stages”
e1“soybean [Glycine max (L.) Merr.] seed development”
Claim · model-written summary
CHS7, CHS8 and IFS2 expression correlated with isoflavone accumulation under well-watered conditions.
- Taxon
- soybeanGlycine maxe1
- Treatment
- well-watered conditionse0
- Gene
- CHS7, CHS8 and IFS2e0
- Outcome
- isoflavone accumulatione0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of CHS7, CHS8 and IFS2 correlated with isoflavone accumulation under well-watered conditions.”
e1“soybean [Glycine max (L.) Merr.] seed development”
Claim · model-written summary
IFS1 transcript abundance remained constant.
- Taxon
- soybeanGlycine maxe1
- Gene
- IFS1e0
- Outcome
- abundance of IFS1 transcriptse0
- Direction
- maintained at a constant ratee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The abundance of IFS1 transcripts was maintained at a constant rate”
e1“soybean [Glycine max (L.) Merr.] seed development”
Claim · model-written summary
IFS2 was down-regulated under water deficit and well-watered conditions.
- Taxon
- soybeanGlycine maxe1
- Gene
- IFS2e0
- Outcome
- IFS2e0
- Direction
- down-regulatede0
- Qualifier
- under both water deficit and well-watered conditionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“IFS2 was down-regulated and highly correlated with isoflavone accumulation under both water deficit and well-watered conditions”
e1“soybean [Glycine max (L.) Merr.] seed development”
Claim · model-written summary
IFS2 expression was highly correlated with isoflavone accumulation under both water regimes.
- Taxon
- soybeanGlycine maxe1
- Gene
- IFS2e0
- Outcome
- isoflavone accumulatione0
- Qualifier
- under both water deficit and well-watered conditionse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“IFS2 was down-regulated and highly correlated with isoflavone accumulation under both water deficit and well-watered conditions”
e1“soybean [Glycine max (L.) Merr.] seed development”
Claim · model-written summary
Authors suggest IFS2 is a main contributor to isoflavone diminution under drought.
- Taxon
- soybeanGlycine maxe1
- Gene
- IFS2e0
- Outcome
- isoflavone diminution under droughte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“suggesting IFS2 as a main contributor to isoflavone diminution under drought”
e1“soybean [Glycine max (L.) Merr.] seed development”
The same questions in other papers
drought · molecular: metabolites · Glycine max (soybean)Positive and negative results
39 papers in the same direction as this paper · 20 in the opposite direction
- positive
- negative
- negative
56 papers more in this group
See all 60 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.