Abstract
Heterologous expression of a cDNA encoding a 62-kD soybean sucrose binding protein in yeast demonstrates that this protein, independent of other plant proteins, mediates sucrose uptake across the plasma membrane. Sucrose binding protein-mediated sucrose uptake is nonsaturable up to 30 mM sucrose, is specific for sucrose, and is relatively insensitive to treatment with sulfhydryl-modifying reagents. Alteration of the external pH or pretreatment of the yeast cells with protonophores did not significantly affect the rate of 14C-sucrose uptake. This demonstrates that sucrose binding protein-mediated sucrose uptake is not dependent on H+ movement and delineates it from other plant sucrose transporters. Physiological characterization of sucrose uptake into higher plant cells has shown the presence of both saturable and nonsaturable uptake components. The nonsaturable mechanism is relatively insensitive to external pH, pretreatment with protonophores, and treatment with sulfhydryl-modifying reagents. Sucrose binding protein-mediated sucrose uptake in yeast mimics this physiologically described, but mechanistically undefined, nonsaturable sucrose uptake mechanism in higher plants. Functional characterization of the sucrose binding protein thus defines both a novel component of sucrose uptake and provides important insight into this nonsaturable sucrose uptake mechanism, which has remained enigmatic since its physiological description.
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Claims 8
Claim · model-written summary
Soybean sucrose binding protein independently mediated sucrose uptake across the yeast plasma membrane.
- Taxon
- soybean
Glycine maxe0 - Gene
- soybean sucrose binding proteine0
- Outcome
- sucrose uptake across the plasma membranee0
- Qualifier
- independent of other plant proteinse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Heterologous expression of a cDNA encoding a 62-kD soybean sucrose binding protein in yeast demonstrates that this protein, independent of other plant proteins, mediates sucrose uptake across the plasma membrane”
Claim · model-written summary
Protein-mediated sucrose uptake was nonsaturable up to 30 mM sucrose.
- Taxon
- soybeanGlycine maxt0
- Gene
- Sucrose binding proteine0
- Outcome
- sucrose uptake is nonsaturablee0
- Value
- 30 mM sucrosee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“soybean”
e0“Sucrose binding protein-mediated sucrose uptake is nonsaturable up to 30 mM sucrose”
Claim · model-written summary
Protein-mediated uptake was specific for sucrose.
- Taxon
- soybeanGlycine maxt0
- Outcome
- specific for sucrosee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“soybean”
e0“is specific for sucrose”
Claim · model-written summary
Protein-mediated sucrose uptake was relatively insensitive to sulfhydryl-modifying reagents.
- Taxon
- soybeanGlycine maxt0
- Treatment
- treatment with sulfhydryl-modifying reagentse0
- Outcome
- relatively insensitivee0
- Direction
- relatively insensitivee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“soybean”
e0“is relatively insensitive to treatment with sulfhydryl-modifying reagents”
Claim · model-written summary
Changing external pH did not significantly affect 14C-sucrose uptake.
- Taxon
- soybeanGlycine maxt0
- Treatment
- Alteration of the external pHe0
- Outcome
- rate of 14C-sucrose uptakee0
- Direction
- did not significantly affecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“soybean”
e0“Alteration of the external pH or pretreatment of the yeast cells with protonophores did not significantly affect the rate of 14C-sucrose uptake”
Claim · model-written summary
Protonophore pretreatment did not significantly affect 14C-sucrose uptake.
- Taxon
- soybeanGlycine maxt0
- Treatment
- pretreatment of the yeast cells with protonophorese0
- Outcome
- rate of 14C-sucrose uptakee0
- Direction
- did not significantly affecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“soybean”
e0“Alteration of the external pH or pretreatment of the yeast cells with protonophores did not significantly affect the rate of 14C-sucrose uptake”
Claim · model-written summary
The authors infer that soybean sucrose binding protein mediated uptake does not depend on H+ movement.
- Taxon
- soybeanGlycine maxt0
- Gene
- sucrose binding proteine0
- Outcome
- sucrose uptake is not dependent on H+ movemente0
- Qualifier
- H+ movemente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“soybean”
e0“sucrose binding protein-mediated sucrose uptake is not dependent on H+ movement”
Claim · model-written summary
The uptake mechanism in yeast mimicked the described nonsaturable uptake mechanism in higher plants.
- Taxon
- soybeanGlycine maxt0
- Outcome
- mimics this physiologically described, but mechanistically undefined, nonsaturable sucrose uptake mechanism in higher plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“soybean”
e0“Sucrose binding protein-mediated sucrose uptake in yeast mimics this physiologically described, but mechanistically undefined, nonsaturable sucrose uptake mechanism in higher plants”
The same questions in other papers
ph · nutrient: content · Glycine max (soybean)Positive and negative results
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- positive
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See all 14 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.