Abstract
The herbicide glyphosate is effectively detoxified by N-acetylation. We screened a collection of microbial isolates and discovered enzymes exhibiting glyphosate N-acetyltransferase (GAT) activity. Kinetic properties of the discovered enzymes were insufficient to confer glyphosate tolerance to transgenic organisms. Eleven iterations of DNA shuffling improved enzyme efficiency by nearly four orders of magnitude from 0.87 mM-1 min-1 to 8320 mM-1 min-1. From the fifth iteration and beyond, GAT enzymes conferred increasing glyphosate tolerance to Escherichia coli, Arabidopsis, tobacco, and maize. Glyphosate acetylation provides an alternative strategy for supporting glyphosate use on crops.
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Claims 5
Claim · model-written summary
Microbial isolates yielded enzymes with glyphosate N-acetyltransferase activity.
Not reportedTaxon
Verbatim
e0“We screened a collection of microbial isolates and discovered enzymes exhibiting glyphosate N-acetyltransferase (GAT) activity”
Claim · model-written summary
DNA shuffling improved enzyme efficiency nearly four orders of magnitude.
- Treatment
- Eleven iterations of DNA shufflinge0
- Outcome
- enzyme efficiencye0
- Direction
- improvede0
- Value
- nearly four orders of magnitudee0
Not reportedTaxonComparator
Verbatim
e0“Eleven iterations of DNA shuffling improved enzyme efficiency by nearly four orders of magnitude from 0.87 mM-1 min-1 to 8320 mM-1 min-1”
Claim · model-written summary
Later GAT enzymes conferred increasing glyphosate tolerance to Arabidopsis.
- Taxon
- Arabidopsis
Arabidopsise0 - Treatment
- GAT enzymese0
- Outcome
- glyphosate tolerancee0
- Direction
- increasinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“From the fifth iteration and beyond, GAT enzymes conferred increasing glyphosate tolerance to Escherichia coli, Arabidopsis, tobacco, and maize”
Claim · model-written summary
Later GAT enzymes conferred increasing glyphosate tolerance to tobacco.
- Taxon
- tobacco
Nicotiana tabacume0 - Treatment
- GAT enzymese0
- Outcome
- glyphosate tolerancee0
- Direction
- increasinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“From the fifth iteration and beyond, GAT enzymes conferred increasing glyphosate tolerance to Escherichia coli, Arabidopsis, tobacco, and maize”
Claim · model-written summary
Later GAT enzymes conferred increasing glyphosate tolerance to maize.
Zea maysNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“From the fifth iteration and beyond, GAT enzymes conferred increasing glyphosate tolerance to Escherichia coli, Arabidopsis, tobacco, and maize”
The same questions in other papers
glyphosate · disease · Nicotiana tabacum (tobacco)Positive and negative results
1 paper in the same direction as this paper · 3 in the opposite direction
- negative
- no effect
- no effect
1 paper more in this group
See all 5 papers →glyphosate · disease · Zea mays (maize)Results agree
5 papers in the same direction as this paper
- positive
- positive
- positive
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.