Abstract
A glyphosate-tolerant tobacco cell-line, Nicotiana tabacum L. Indiana 7, was selected from the glyphosate-sensitive Wisconsin 38 line. Tolerance and growth characteristics of Indiana 7 cells were the same for cells maintained for more than one year in the presence or absence of glyphosate. Wisconsin 38 cells accumulated 46 times more shikimate than Indiana 7 cells, when cells of both lines were exposed to the herbicide. Specific activities of 3-deoxy- sc D-arabino-heptulosonate 7-phosphate (DAHP) synthase, the first enzyme of the shikimate pathway, and 5-enolpyruvylshikimate 3-phosphate synthase, the target site of glyphosate, were elevated in Indiana 7 cells. Plants regenerated from Indiana 7 cells retained tolerance to glyphosate. A cDNA encoding potato DAHP synthase, the first from any eukaryotic source, was cloned into $\lambda$gt11. The nucleotide suquence was obtained, and the identity of the cDNA was confirmed through partial amino acid sequence analysis of DAHP synthase. The cDNA contains a 1521 bp open reading frame that encodes a polypeptide with a calculated molecular weight of 56,108. The N-terminus of the deduced amino acid sequence of potato DAHP synthase resembles a transit peptide that has regions of charged amino acid residues in common with other chloroplast transit peptide sequences. Amino acid sequence identity between the mature potato DAHP synthase and the E. coli Phe-sensitive DAHP synthase is 27%. Wounding potato tubers induced steady-state levels of translatable DAHP synthase mRNA seven-fold. The mRNA increase was followed by a comparable elevation of enzyme activity and amount. The induction was localized to the wound site, but was maximal in periderm tissue, indicating tissue-specific regulation within the potato tuber. DAHP synthase was also wound-induced in potato leaves and tomato fruit. The regulation of DAHP synthase in response to stress conditions confirms its role as a key regulatory enzyme of the shikimate pathway.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 17
Claim · model-written summary
Tolerance and growth of Indiana 7 cells were the same whether or not they were maintained on glyphosate for more than one year.
- Taxon
- tobacco
Nicotiana tabacume2 - Material
- Indiana 7e1
- Treatment
- glyphosatee1
- Outcome
- tolerance and growth characteristicse1
- Direction
- the samee1
- Comparator
- absence of glyphosatee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Tolerance and growth characteristics of Indiana 7 cells were the same for cells maintained for more than one year in the presence or absence of glyphosate”
e2“A glyphosate-tolerant tobacco cell-line, Nicotiana tabacum L. Indiana 7, was selected from the glyphosate-sensitive Wisconsin 38 line”
Claim · model-written summary
Wisconsin 38 cells accumulated 46 times more shikimate than Indiana 7 cells when both lines were exposed to the herbicide.
- Taxon
- tobaccoNicotiana tabacume2
- Material
- Wisconsin 38e1
- Treatment
- exposed to the herbicidee1
- Outcome
- shikimatee1
- Direction
- moree1
- Value
- 46 timese1
- Comparator
- Indiana 7e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Wisconsin 38 cells accumulated 46 times more shikimate than Indiana 7 cells, when cells of both lines were exposed to the herbicide”
e2“A glyphosate-tolerant tobacco cell-line, Nicotiana tabacum L. Indiana 7, was selected from the glyphosate-sensitive Wisconsin 38 line”
Claim · model-written summary
Specific activity of DAHP synthase was elevated in Indiana 7 cells.
- Taxon
- tobaccoNicotiana tabacume2
- Material
- Indiana 7e1
- Gene
- 3-deoxy- sc D-arabino-heptulosonate 7-phosphate (DAHP) synthasee1
- Outcome
- Specific activitiese1
- Direction
- elevatede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Specific activities of 3-deoxy- sc D-arabino-heptulosonate 7-phosphate (DAHP) synthase, the first enzyme of the shikimate pathway, and 5-enolpyruvylshikimate 3-phosphate synthase, the target site of glyphosate, were elevated in Indiana 7 cells”
e2“A glyphosate-tolerant tobacco cell-line, Nicotiana tabacum L. Indiana 7, was selected from the glyphosate-sensitive Wisconsin 38 line”
Claim · model-written summary
Specific activity of 5-enolpyruvylshikimate 3-phosphate synthase, the glyphosate target, was elevated in Indiana 7 cells.
- Taxon
- tobaccoNicotiana tabacume2
- Material
- Indiana 7e1
- Gene
- 5-enolpyruvylshikimate 3-phosphate synthasee1
- Outcome
- Specific activitiese1
- Direction
- elevatede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Specific activities of 3-deoxy- sc D-arabino-heptulosonate 7-phosphate (DAHP) synthase, the first enzyme of the shikimate pathway, and 5-enolpyruvylshikimate 3-phosphate synthase, the target site of glyphosate, were elevated in Indiana 7 cells”
e2“A glyphosate-tolerant tobacco cell-line, Nicotiana tabacum L. Indiana 7, was selected from the glyphosate-sensitive Wisconsin 38 line”
Claim · model-written summary
Plants regenerated from Indiana 7 cells retained tolerance to glyphosate.
- Taxon
- tobaccoNicotiana tabacume2
- Material
- Indiana 7e1
- Outcome
- tolerance to glyphosatee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Plants regenerated from Indiana 7 cells retained tolerance to glyphosate”
e2“A glyphosate-tolerant tobacco cell-line, Nicotiana tabacum L. Indiana 7, was selected from the glyphosate-sensitive Wisconsin 38 line”
Claim · model-written summary
A cDNA encoding potato DAHP synthase was cloned, the first from any eukaryotic source.
- Taxon
- potato
Solanum tuberosume1 - Gene
- DAHP synthasee1
- Outcome
- cDNA encoding potato DAHP synthasee1
- Qualifier
- the first from any eukaryotic sourcee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“A cDNA encoding potato DAHP synthase, the first from any eukaryotic source, was cloned into”
Claim · model-written summary
The identity of the potato DAHP synthase cDNA was confirmed by partial amino acid sequence analysis.
- Taxon
- potatoSolanum tuberosume2
- Gene
- DAHP synthasee1
- Outcome
- identity of the cDNAe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“the identity of the cDNA was confirmed through partial amino acid sequence analysis of DAHP synthase”
e2“A cDNA encoding potato DAHP synthase, the first from any eukaryotic source, was cloned into”
Claim · model-written summary
The potato DAHP synthase cDNA contains a 1521 bp open reading frame.
- Taxon
- potatoSolanum tuberosume2
- Outcome
- open reading framee1
- Value
- 1521 bpe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The cDNA contains a 1521 bp open reading frame that encodes a polypeptide with a calculated molecular weight of 56,108”
e2“A cDNA encoding potato DAHP synthase, the first from any eukaryotic source, was cloned into”
Claim · model-written summary
The calculated molecular weight of the potato DAHP synthase polypeptide is 56,108.
- Taxon
- potatoSolanum tuberosume2
- Outcome
- calculated molecular weighte1
- Value
- 56,108e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The cDNA contains a 1521 bp open reading frame that encodes a polypeptide with a calculated molecular weight of 56,108”
e2“A cDNA encoding potato DAHP synthase, the first from any eukaryotic source, was cloned into”
Claim · model-written summary
The N-terminus of potato DAHP synthase resembles a chloroplast transit peptide.
- Taxon
- potatoSolanum tuberosume1
- Gene
- DAHP synthasee1
- Outcome
- N-terminus of the deduced amino acid sequencee1
- Qualifier
- resembles a transit peptide that has regions of charged amino acid residues in common with other chloroplast transit peptide sequencese1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The N-terminus of the deduced amino acid sequence of potato DAHP synthase resembles a transit peptide that has regions of charged amino acid residues in common with other chloroplast transit peptide sequences”
Claim · model-written summary
Mature potato DAHP synthase shares 27% amino acid sequence identity with the E. coli Phe-sensitive DAHP synthase.
- Taxon
- potatoSolanum tuberosume1
- Gene
- DAHP synthasee1
- Outcome
- Amino acid sequence identitye1
- Value
- 27%e1
- Comparator
- E. coli Phe-sensitive DAHP synthasee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Amino acid sequence identity between the mature potato DAHP synthase and the E. coli Phe-sensitive DAHP synthase is 27%”
Claim · model-written summary
Wounding of potato tubers induced steady-state DAHP synthase mRNA levels seven-fold.
- Taxon
- potatoSolanum tuberosume1
- Organ / stage
- tuberse1
- Treatment
- Woundinge1
- Gene
- DAHP synthasee1
- Outcome
- steady-state levels of translatable DAHP synthase mRNAe1
- Direction
- inducede1
- Value
- seven-folde1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Wounding potato tubers induced steady-state levels of translatable DAHP synthase mRNA seven-fold”
Claim · model-written summary
Wounding was followed by a comparable elevation of DAHP synthase enzyme activity and amount in potato.
- Taxon
- potatoSolanum tuberosume2
- Treatment
- Woundinge2
- Outcome
- enzyme activity and amounte1
- Direction
- elevatione1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The mRNA increase was followed by a comparable elevation of enzyme activity and amount”
e2“Wounding potato tubers induced steady-state levels of translatable DAHP synthase mRNA seven-fold”
Claim · model-written summary
Wound-induced DAHP synthase induction was localized to the wound site and was maximal in potato periderm tissue.
- Taxon
- potatoSolanum tuberosume1
- Organ / stage
- periderm tissuee1
- Outcome
- inductione1
- Direction
- maximale1
- Qualifier
- localized to the wound sitee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The induction was localized to the wound site, but was maximal in periderm tissue, indicating tissue-specific regulation within the potato tuber”
Claim · model-written summary
DAHP synthase was wound-induced in potato leaves.
- Taxon
- potatoSolanum tuberosume1
- Organ / stage
- leavese1
- Treatment
- wounde1
- Gene
- DAHP synthasee1
- Outcome
- DAHP synthasee1
- Direction
- wound-inducede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“DAHP synthase was also wound-induced in potato leaves and tomato fruit”
Claim · model-written summary
DAHP synthase was wound-induced in tomato fruit.
- Taxon
- tomato
Solanum lycopersicume1 - Organ / stage
- fruite1
- Treatment
- wounde1
- Gene
- DAHP synthasee1
- Outcome
- DAHP synthasee1
- Direction
- wound-inducede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“DAHP synthase was also wound-induced in potato leaves and tomato fruit”
Claim · model-written summary
The wound-responsive regulation of DAHP synthase confirms its role as a key regulatory enzyme of the shikimate pathway.
- Taxon
- potatoSolanum tuberosume2
- Gene
- DAHP synthasee1
- Outcome
- role as a key regulatory enzyme of the shikimate pathwaye1
- Qualifier
- in response to stress conditionse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The regulation of DAHP synthase in response to stress conditions confirms its role as a key regulatory enzyme of the shikimate pathway”
e2“A cDNA encoding potato DAHP synthase, the first from any eukaryotic source, was cloned into”
The same questions in other papers
glyphosate · disease · Nicotiana tabacum (tobacco)Positive and negative results
2 papers in the same direction as this paper · 2 in the opposite direction
- positive
- positive
- negative
1 paper more in this group
See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.