Abstract
Thiamine (vitamin B1, VB1) can act as a plant defence trigger, or priming agent, leading to a rapid counterattack on pathogen invasion. In this study, the priming effect of thiamine on rice (Oryza sativa cv. Nipponbare) and its activity against root-knot nematode (Meloidogyne graminicola) infection were evaluated. Thiamine treatment and subsequent nematode inoculation activated hydrogen peroxide (H2O2) accumulation and lignin deposition in plant roots, and this correlated with enhanced transcription of OsPAL1 and OsC4H, two genes involved in the phenylpropanoid pathway. The number of nematodes in rice roots was slightly but significantly reduced, and the development of the nematodes was delayed, whereas no direct toxic effects of VB1 on nematode viability and infectivity were observed. The combined application of thiamine with l-2-aminooxy-3-phenylpropionic acid (AOPP), an inhibitor of phenylalanine ammonia-lyase (PAL), significantly hampered the VB1-priming capacity. These findings indicate that thiamine-induced priming in rice involves H2O2 and phenylpropanoid-mediated lignin production, which hampers nematode infection. Further cellular and molecular studies on the mechanism of thiamine-induced defence will be useful for the development of novel nematode control strategies.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 7
Claim · model-written summary
Thiamine treatment followed by nematode inoculation activated hydrogen peroxide accumulation and lignin deposition.
- Taxon
- rice
Oryza sativat0 - Organ / stage
- plant rootse0
- Treatment
- Thiamine treatment and subsequent nematode inoculatione0
- Interactor
- Meloidogyne graminicolaMeloidogyne graminicolae1peste0
- Outcome
- hydrogen peroxide (H2O2) accumulation and lignin depositione0
- Direction
- activatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Thiamine treatment and subsequent nematode inoculation activated hydrogen peroxide (H2O2) accumulation and lignin deposition in plant roots”
t0“rice”
e1“In this study, the priming effect of thiamine on rice (Oryza sativa cv. Nipponbare) and its activity against root-knot nematode (Meloidogyne graminicola) infection were evaluated.”
Claim · model-written summary
Defense response correlated with enhanced transcription of OsPAL1 and OsC4H.
- Taxon
- riceOryza sativat0
- Gene
- OsPAL1 and OsC4He0
- Outcome
- enhanced transcription of OsPAL1 and OsC4He0
- Direction
- enhancede0
- Qualifier
- phenylpropanoid pathwaye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“this correlated with enhanced transcription of OsPAL1 and OsC4H, two genes involved in the phenylpropanoid pathway”
t0“rice”
e1“In this study, the priming effect of thiamine on rice (Oryza sativa cv. Nipponbare) and its activity against root-knot nematode (Meloidogyne graminicola) infection were evaluated.”
Claim · model-written summary
Thiamine slightly but significantly reduced nematode numbers in rice roots.
- Taxon
- riceOryza sativat0
- Organ / stage
- rice rootse0
- Treatment
- Thiaminee1
- Interactor
- Meloidogyne graminicolaMeloidogyne graminicolae1peste0
- Outcome
- number of nematodes in rice rootse0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The number of nematodes in rice roots was slightly but significantly reduced”
t0“rice”
e1“In this study, the priming effect of thiamine on rice (Oryza sativa cv. Nipponbare) and its activity against root-knot nematode (Meloidogyne graminicola) infection were evaluated.”
Claim · model-written summary
Nematode development was delayed.
- Taxon
- riceOryza sativat0
- Treatment
- Thiaminee1
- Interactor
- Meloidogyne graminicolaMeloidogyne graminicolae1peste0
- Outcome
- development of the nematodese0
- Direction
- delayede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the development of the nematodes was delayed”
t0“rice”
e1“In this study, the priming effect of thiamine on rice (Oryza sativa cv. Nipponbare) and its activity against root-knot nematode (Meloidogyne graminicola) infection were evaluated.”
Claim · model-written summary
VB1 showed no direct toxicity to nematode viability or infectivity.
- Taxon
- riceOryza sativat0
- Treatment
- VB1e0
- Interactor
- Meloidogyne graminicolaMeloidogyne graminicolae1peste0
- Outcome
- nematode viability and infectivitye0
- Direction
- no direct toxic effectse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“no direct toxic effects of VB1 on nematode viability and infectivity were observed”
t0“rice”
e1“In this study, the priming effect of thiamine on rice (Oryza sativa cv. Nipponbare) and its activity against root-knot nematode (Meloidogyne graminicola) infection were evaluated.”
Claim · model-written summary
Adding AOPP significantly hampered VB1 priming capacity.
- Taxon
- riceOryza sativat0
- Treatment
- combined application of thiamine with l-2-aminooxy-3-phenylpropionic acid (AOPP)e0
- Outcome
- VB1-priming capacitye0
- Direction
- hamperede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The combined application of thiamine with l-2-aminooxy-3-phenylpropionic acid (AOPP), an inhibitor of phenylalanine ammonia-lyase (PAL), significantly hampered the VB1-priming capacity”
t0“rice”
e1“In this study, the priming effect of thiamine on rice (Oryza sativa cv. Nipponbare) and its activity against root-knot nematode (Meloidogyne graminicola) infection were evaluated.”
Claim · model-written summary
Authors inferred that hydrogen peroxide and lignin production mediate priming that hampers nematode infection.
- Taxon
- riceOryza sativat0
- Interactor
- Meloidogyne graminicolaMeloidogyne graminicolae1peste0
- Outcome
- nematode infectione0
- Direction
- hamperse0
- Qualifier
- H2O2 and phenylpropanoid-mediated lignin productione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“thiamine-induced priming in rice involves H2O2 and phenylpropanoid-mediated lignin production, which hampers nematode infection”
t0“rice”
e1“In this study, the priming effect of thiamine on rice (Oryza sativa cv. Nipponbare) and its activity against root-knot nematode (Meloidogyne graminicola) infection were evaluated.”
The same questions in other papers
inoculation · Meloidogyne graminicola · Oryza sativa (rice)Positive and negative results
7 papers in the same direction as this paper · 5 in the opposite direction
- negative
- negative
- negative
9 papers more in this group
See all 13 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.