Abstract
The Arabidopsis thaliana VERNALIZATION INDEPENDENCE (VIP) gene class has multiple functions in development, including repression of flowering through activation of the MADSbox gene FLC. Epigenetic silencing of FLC plays a substantial role in the promotion of flowering through cold (vernalization). To better understand how VIP genes influence development, we undertook a genetic and molecular study of the previously uncharacterized VIP5 and VIP6 genes. We found that loss of function of these genes also resulted in downregulation of other members of the FLC/MAF gene family, including the photoperiodic pathway regulator MAF1/FLM. We cloned VIP5 and VIP6 through mapping and transcriptional profiling. Both proteins are closely related to distinct components of budding yeast Paf1C, a transcription factor that assists in establishment and maintenance of transcription-promotive chromatin modifications such as ubiquitination of H2B by Bre1/Rad6 and methylation of histone H3 lysine-4 by the trithorax-related histone methylase Set1. Genetic analysis and coimmunoprecipitation experiments suggest that VIP5 and VIP6 function in the same mechanism as the previously described VIP3 and VIP4. Our findings suggest that an evolutionarily conserved transcriptional mechanism plays an essential role in the maintenance of gene expression in higher eukaryotes and has a central function in flowering.
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Claims 4
Claim · model-written summary
Loss of VIP5 and VIP6 function downregulated FLC/MAF family members including MAF1/FLM.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae0 - Treatment
- loss of functione1
- Gene
- FLC/MAFe1
- Outcome
- other members of the FLC/MAF gene familye1
- Direction
- downregulatione1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis thaliana”
e1“loss of function of these genes also resulted in downregulation of other members of the FLC/MAF gene family, including the photoperiodic pathway regulator MAF1/FLM”
Claim · model-written summary
VIP5 and VIP6 were cloned by mapping and transcriptional profiling.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Gene
- VIP5 and VIP6e1
- Outcome
- cloned VIP5 and VIP6e1
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis thaliana”
e1“We cloned VIP5 and VIP6 through mapping and transcriptional profiling”
Claim · model-written summary
VIP5 and VIP6 proteins are closely related to distinct budding yeast Paf1C components.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Gene
- Paf1Ce1
- Outcome
- closely related to distinct componentse1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis thaliana”
e1“Both proteins are closely related to distinct components of budding yeast Paf1C”
Claim · model-written summary
Genetic and coimmunoprecipitation results suggest VIP5 and VIP6 function in the same mechanism as VIP3 and VIP4.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Gene
- VIP5 and VIP6e1
- Outcome
- function in the same mechanisme1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis thaliana”
e1“Genetic analysis and coimmunoprecipitation experiments suggest that VIP5 and VIP6 function in the same mechanism as the previously described VIP3 and VIP4”
The same questions in other papers
loss of function · molecular: expression · Arabidopsis thaliana (thale cress)Positive and negative results
59 papers in the same direction as this paper · 13 in the opposite direction
- positive
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See all 73 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.