Abstract
Nitric oxide (NO) has been reported to be involved in breaking seed dormancy but its mechanism of action is unclear. Here, we report that a rapid accumulation of NO induced an equally rapid decrease of abscisic acid (ABA) that is required for this action in Arabidopsis. Results of quantitative real-time polymerase chain reaction (QRT-PCR) and Western blotting indicate that the NO-induced ABA decrease correlates with the regulation of CYP707A2 transcription and (+)-abscisic acid 8'-hydroxylase (encoded by CYP707A2) protein expression. By analysing cyp707a1, cyp707a2 and cyp707a3 mutants, we found that CYP707A2 plays a major role in ABA catabolism during the first stage of imbibition. Fluorescent images demonstrate that NO is released rapidly in the early hours at the endosperm layer during imbibition. Evidently, such response precedes the enhancement of ABA catabolism which is required for subsequent seed germination.
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Claims 6
Claim · model-written summary
Rapid NO accumulation induced a rapid ABA decrease required for dormancy breaking.
- Taxon
- Arabidopsis
Arabidopsise0 - Compound
- NOe0
- Outcome
- decrease of abscisic acid (ABA)e0
- Direction
- decreasee0
Not reportedOrgan / stage
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a rapid accumulation of NO induced an equally rapid decrease of abscisic acid (ABA) that is required for this action in Arabidopsis”
Claim · model-written summary
NO-induced ABA decrease correlated with CYP707A2 transcription regulation.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- CYP707A2e0
- Outcome
- ABA decreasee0
- Direction
- decreasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the NO-induced ABA decrease correlates with the regulation of CYP707A2 transcription”
t0“Arabidopsis”
Claim · model-written summary
NO-induced ABA decrease correlated with hydroxylase protein expression.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- (+)-abscisic acid 8'-hydroxylasee0
- Outcome
- ABA decreasee0
- Direction
- decreasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the NO-induced ABA decrease correlates with the regulation of CYP707A2 transcription and (+)-abscisic acid 8'-hydroxylase (encoded by CYP707A2) protein expression”
t0“Arabidopsis”
Claim · model-written summary
CYP707A2 has a major role in early imbibition ABA catabolism.
- Taxon
- ArabidopsisArabidopsist0
- Organ / stage
- first stage of imbibitione0
- Gene
- CYP707A2e0
- Outcome
- ABA catabolisme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CYP707A2 plays a major role in ABA catabolism during the first stage of imbibition”
t0“Arabidopsis”
Claim · model-written summary
NO was rapidly released at the endosperm layer early in imbibition.
- Taxon
- ArabidopsisArabidopsist0
- Organ / stage
- endosperm layere0
- Outcome
- NO is releasede0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NO is released rapidly in the early hours at the endosperm layer during imbibition”
t0“Arabidopsis”
Claim · model-written summary
Early NO release preceded enhanced ABA catabolism required for subsequent germination.
- Taxon
- ArabidopsisArabidopsist0
- Outcome
- subsequent seed germinatione0
- Qualifier
- enhancement of ABA catabolisme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“such response precedes the enhancement of ABA catabolism which is required for subsequent seed germination”
t0“Arabidopsis”