Abstract
Two C-repeat binding factor genes (EguCBF1a/b), isolated from E. gunnii and differentially cold-regulated, were constitutively overexpressed in a cold-sensitive Eucalyptus hybrid. In addition to the expected improvement on freezing tolerance, some resulting transgenic lines (EguCBF1a-OE and EguCBF1b-OE) exhibited a decrease in stomata density and an over-accumulation of anthocyanins also observed to a lesser extent in a cold-acclimated control plant. Given that the induction of five putative CBF target genes was observed in CBF-overexpressing lines as well as in the cold-acclimated control line, these phenotypes might be related to cold acclimation. In comparison with the control plant, the most altered transgenic line (EguCBF1a-OE A1 line), exhibited reduced growth and better water retention capacity. This modified phenotype includes reduced leaf area and thickness associated with a decrease in cell size, as well as a higher oil gland density and a wax deposition on the cuticle. Surprisingly, the EguCBF1b-OE B9 line, with a level of transgene expression equivalent to the A1 line, showed a less marked phenotype, suggesting a difference in transactivation efficiency between EguCBF1A and B factors. The features of these transgenic lines provide the first signs of adaptive mechanisms controlled by CBF transcription factors in an evergreen broad-leaved tree. These data also open new prospects towards genetic improvement on Eucalyptus for freezing tolerance.
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Claims 10
Claim · model-written summary
CBF overexpression improved freezing tolerance.
- Taxon
- Eucalyptus
.jpg?width=60)
Eucalyptuse1 - Treatment
- constitutively overexpressede1
- Gene
- EguCBF1a-OE and EguCBF1b-OEe0
- Outcome
- freezing tolerancee0
- Direction
- improvemente0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“In addition to the expected improvement on freezing tolerance, some resulting transgenic lines (EguCBF1a-OE and EguCBF1b-OE) exhibited a decrease in stomata density and an over-accumulation of anthocyanins”
e1“were constitutively overexpressed in a cold-sensitive Eucalyptus hybrid”
Claim · model-written summary
Some CBF overexpressing lines had reduced stomatal density.
- Taxon
- EucalyptusEucalyptust0
- Treatment
- transgenic linese0
- Outcome
- stomata densitye0
- Direction
- decreasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“some resulting transgenic lines (EguCBF1a-OE and EguCBF1b-OE) exhibited a decrease in stomata density”
t0“Eucalyptus”
Claim · model-written summary
Some CBF overexpressing lines over accumulated anthocyanins.
- Taxon
- EucalyptusEucalyptust0
- Treatment
- transgenic linese0
- Outcome
- anthocyaninse0
- Direction
- over-accumulatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“some resulting transgenic lines (EguCBF1a-OE and EguCBF1b-OE) exhibited a decrease in stomata density and an over-accumulation of anthocyanins”
t0“Eucalyptus”
Claim · model-written summary
Five putative CBF target genes were induced in overexpressing and cold acclimated control lines.
- Taxon
- EucalyptusEucalyptust0
- Gene
- CBF target genese0
- Outcome
- induction of five putative CBF target genese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the induction of five putative CBF target genes was observed in CBF-overexpressing lines as well as in the cold-acclimated control line”
t0“Eucalyptus”
Claim · model-written summary
The A1 line grew less than the control.
- Taxon
- EucalyptusEucalyptust0
- Treatment
- EguCBF1a-OE A1 linee0
- Outcome
- growthe0
- Direction
- reducede0
- Comparator
- control plante0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In comparison with the control plant, the most altered transgenic line (EguCBF1a-OE A1 line), exhibited reduced growth and better water retention capacity”
t0“Eucalyptus”
Claim · model-written summary
The A1 line retained water better than the control.
- Taxon
- EucalyptusEucalyptust0
- Treatment
- EguCBF1a-OE A1 linee0
- Outcome
- water retention capacitye0
- Direction
- bettere0
- Comparator
- control plante0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In comparison with the control plant, the most altered transgenic line (EguCBF1a-OE A1 line), exhibited reduced growth and better water retention capacity”
t0“Eucalyptus”
Claim · model-written summary
The A1 line had reduced leaf area and thickness associated with smaller cells.
- Taxon
- EucalyptusEucalyptust0
- Organ / stage
- leafe0
- Outcome
- leaf area and thicknesse0
- Direction
- reducede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This modified phenotype includes reduced leaf area and thickness associated with a decrease in cell size”
t0“Eucalyptus”
Claim · model-written summary
The A1 line had higher oil gland density.
- Taxon
- EucalyptusEucalyptust0
- Outcome
- oil gland densitye0
- Direction
- highere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“as well as a higher oil gland density and a wax deposition on the cuticle”
t0“Eucalyptus”
Claim · model-written summary
Wax was deposited on the A1 line cuticle.
- Taxon
- EucalyptusEucalyptust0
- Outcome
- wax deposition on the cuticlee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a wax deposition on the cuticle”
t0“Eucalyptus”
Claim · model-written summary
The B9 line had a less marked phenotype despite equivalent transgene expression to A1.
- Taxon
- EucalyptusEucalyptust0
- Material
- EguCBF1b-OE B9 linee0
- Outcome
- phenotypee0
- Direction
- less markede0
- Comparator
- A1 linee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the EguCBF1b-OE B9 line, with a level of transgene expression equivalent to the A1 line, showed a less marked phenotype”
t0“Eucalyptus”