Abstract
Using freshly isolated maize mesophyll protoplasts and a transient expression method, I showed that the transcriptional activity of seven maize photosynthetic gene promoters is specifically and coordinately repressed by the photosynthetic end products sucrose and glucose and by the exogenous carbon source acetate. Analysis of deleted, mutated, and hybrid promoters showed that sugars and acetate inhibit the activity of distinct positive upstream regulatory elements without a common consensus. The metabolic repression of photosynthetic genes overrides other forms of regulation, e.g., light, tissue type, and developmental stage. Repression by sugars and repression by acetate are mediated by different mechanisms. The identification of conditions that avoid sugar repression overcomes a major obstacle to the study of photosynthetic gene regulation in higher plants.
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Claims 5
Claim · model-written summary
Sucrose and glucose repress the transcriptional activity of seven maize photosynthetic gene promoters.
- Treatment
- sucrose and glucosee1
- Gene
- maize photosynthetic gene promoterse1
- Outcome
- transcriptional activitye1
- Direction
- repressede1
Zea maysNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Using freshly isolated maize mesophyll protoplasts”
e1“the transcriptional activity of seven maize photosynthetic gene promoters is specifically and coordinately repressed by the photosynthetic end products sucrose and glucose and by the exogenous carbon source acetate”
Claim · model-written summary
Acetate, an exogenous carbon source, represses the transcriptional activity of seven maize photosynthetic gene promoters.
- Treatment
- acetatee1
- Gene
- maize photosynthetic gene promoterse1
- Outcome
- transcriptional activitye1
- Direction
- repressede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Using freshly isolated maize mesophyll protoplasts”
e1“the transcriptional activity of seven maize photosynthetic gene promoters is specifically and coordinately repressed by the photosynthetic end products sucrose and glucose and by the exogenous carbon source acetate”
Claim · model-written summary
Sugars and acetate inhibit the activity of distinct positive upstream regulatory elements without a common consensus.
- Treatment
- sugars and acetatee1
- Outcome
- distinct positive upstream regulatory elementse1
- Qualifier
- without a common consensuse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Using freshly isolated maize mesophyll protoplasts”
e1“sugars and acetate inhibit the activity of distinct positive upstream regulatory elements without a common consensus”
Claim · model-written summary
Metabolic repression of photosynthetic genes overrides other forms of regulation such as light, tissue type and developmental stage.
- Gene
- photosynthetic genese1
- Outcome
- overrides other forms of regulatione1
- Qualifier
- light, tissue type, and developmental stagee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Using freshly isolated maize mesophyll protoplasts”
e1“The metabolic repression of photosynthetic genes overrides other forms of regulation, e.g., light, tissue type, and developmental stage”
Claim · model-written summary
Repression by sugars and repression by acetate are mediated by different mechanisms.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Using freshly isolated maize mesophyll protoplasts”
e1“Repression by sugars and repression by acetate are mediated by different mechanisms”
The same questions in other papers
glucose · molecular: expression · Zea mays (maize)Positive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction · 1 reporting no difference from an alternative
- positive
- no effect
- no effect
sucrose · molecular: expression · Zea mays (maize)Positive and negative results
5 papers in the same direction as this paper · 2 in the opposite direction
- negative
- no effect
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4 papers more in this group
See all 8 papers →acetate · molecular: expression · Zea mays (maize)Results agree
2 papers in the same direction as this paper
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.