Abstract
Plant expression of the entomopathogenic bacteria Bacillus thuringiensis cry gene has reduced the damage created by insect pests in several economically important cultures. For this study, we have conducted genetic transformation of the indica rice "IRGA 424", via Agrobacterium tumefaciens, using the B. thuringiensis cry1Aa and cry1B genes, with the objective of obtaining rice plants resistant to the insect pests from this culture. The gene constructions harbor the promoters maize proteinase inhibitor and ubiquitin. The results showed that high concentration of the hormone 2,4-dichlorophenoxyacetic acid and agarose as the gelling agent helped the production of embryogenic calli for the analyzed cultivar. More than 80% of the obtained transformed plants revealed the integration, using polymerase chain reaction, of the cry1Aa and cry1B genes. Analysis of the expression of the heterologous protein by Western blotting revealed the expression of the Cry1B delta-endotoxin in IRGA 424 plants transformed with the ubiquitin promoter. Data showed the production and dissemination of a high number of embryogenic calli in addition to obtaining plants transformed with the cry1Aa and cry1B genes until the reproductive phase. The feed bioassays with the transformed plants and Spodoptera frugiperda (JE Smith) larvae indicated high rates of mortality to the insect target. The highest corrected mortality rate achieved under laboratory conditions with Bt-rice plants transformed with the cry1B and cry1Aa genes was 94 and 84%, respectively. Thus, our results demonstrated the great potential of transformed Bt-rice plants in controlling the damage caused by these insect pests in rice paddy fields.
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Claims 9
Claim · model-written summary
High 2,4-D concentration and agarose as gelling agent helped the production of embryogenic calli in indica rice IRGA 424.
- Taxon
- indica rice
Oryza sativa Indica Groupe1 - Material
- IRGA 424e1
- Treatment
- high concentration of the hormone 2,4-dichlorophenoxyacetic acid and agarose as the gelling agente2
- Outcome
- production of embryogenic callie2
- Direction
- helpede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“genetic transformation of the indica rice "IRGA 424"”
e2“The results showed that high concentration of the hormone 2,4-dichlorophenoxyacetic acid and agarose as the gelling agent helped the production of embryogenic calli for the analyzed cultivar”
Claim · model-written summary
More than 80% of the obtained transformed indica rice plants showed integration of the cry1Aa and cry1B genes by PCR.
- Taxon
- indica riceOryza sativa Indica Groupe1
- Gene
- cry1Aa and cry1B genese3
- Outcome
- integratione3
- Value
- More than 80%e3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“genetic transformation of the indica rice "IRGA 424"”
e3“More than 80% of the obtained transformed plants revealed the integration, using polymerase chain reaction, of the cry1Aa and cry1B genes”
Claim · model-written summary
Cry1B delta-endotoxin is expressed in IRGA 424 plants transformed with the ubiquitin promoter, as shown by Western blotting.
- Taxon
- indica riceOryza sativa Indica Groupe1
- Material
- IRGA 424e4
- Treatment
- transformed with the ubiquitin promotere4
- Gene
- Cry1B delta-endotoxine4
- Outcome
- expressione4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“genetic transformation of the indica rice "IRGA 424"”
e4“Analysis of the expression of the heterologous protein by Western blotting revealed the expression of the Cry1B delta-endotoxin in IRGA 424 plants transformed with the ubiquitin promoter”
Claim · model-written summary
A high number of embryogenic calli was produced and disseminated in indica rice IRGA 424.
- Taxon
- indica riceOryza sativa Indica Groupe1
- Outcome
- production and dissemination of a high number of embryogenic callie5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“genetic transformation of the indica rice "IRGA 424"”
e5“Data showed the production and dissemination of a high number of embryogenic calli in addition to obtaining plants transformed with the cry1Aa and cry1B genes until the reproductive phase”
Claim · model-written summary
Plants transformed with cry1Aa and cry1B were obtained up to the reproductive phase in indica rice IRGA 424.
- Taxon
- indica riceOryza sativa Indica Groupe1
- Organ / stage
- reproductive phasee5
- Outcome
- obtaining plants transformed with the cry1Aa and cry1B genese5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“genetic transformation of the indica rice "IRGA 424"”
e5“Data showed the production and dissemination of a high number of embryogenic calli in addition to obtaining plants transformed with the cry1Aa and cry1B genes until the reproductive phase”
Claim · model-written summary
Transformed rice plants gave high mortality of Spodoptera frugiperda larvae in feed bioassays.
- Taxon
- indica riceOryza sativa Indica Groupe1
- Treatment
- transformed plantse6
- Interactor
- Spodoptera frugiperda
.jpg?width=60)
Spodoptera frugiperdae6pest - Outcome
- mortality to the insect targete6
- Direction
- highe6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e1“genetic transformation of the indica rice "IRGA 424"”
e6“The feed bioassays with the transformed plants and Spodoptera frugiperda (JE Smith) larvae indicated high rates of mortality to the insect target”
Claim · model-written summary
Bt-rice plants transformed with cry1B reached a highest corrected mortality rate of 94% under laboratory conditions.
- Taxon
- indica riceOryza sativa Indica Groupe1
- Treatment
- Bt-rice plants transformed with the cry1Be7
- Outcome
- highest corrected mortality ratee7
- Direction
- higheste7
- Value
- 94e7
- Setting
- laboratory conditionse7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“genetic transformation of the indica rice "IRGA 424"”
e7“The highest corrected mortality rate achieved under laboratory conditions with Bt-rice plants transformed with the cry1B and cry1Aa genes was 94 and 84%, respectively”
Claim · model-written summary
Bt-rice plants transformed with cry1Aa reached a highest corrected mortality rate of 84% under laboratory conditions.
- Taxon
- indica riceOryza sativa Indica Groupe1
- Treatment
- cry1Aae7
- Outcome
- highest corrected mortality ratee7
- Direction
- higheste7
- Value
- 84%e7
- Setting
- laboratory conditionse7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“genetic transformation of the indica rice "IRGA 424"”
e7“The highest corrected mortality rate achieved under laboratory conditions with Bt-rice plants transformed with the cry1B and cry1Aa genes was 94 and 84%, respectively”
Claim · model-written summary
The authors recommend transformed Bt-rice plants as a potential means of controlling insect pest damage in rice paddy fields.
Verbatim
e0“our results demonstrated the great potential of transformed Bt-rice plants in controlling the damage caused by these insect pests in rice paddy fields”
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