Abstract
Abstract Proteins of hypocotyls of bean were studied by electrophoresis. Proteins were extracted from hypocotyl segments of various stages of development starting with the relatively undifferentiated hook regions and proceeding by 2 cm segments down the hypocotyl. The proteins were the soluble (pH 7.4), the basic nuclear (histones), acidic ribonuclear and acidic chromosomal. Soluble proteins reflected differentiation of the hypocotyl in that lower hypocotyl segments had more different protein types than did the hook region. Indoleacetic acid (IAA) at 10 −6 M when applied to the lower hypocotyl appeared to induce still more different proteins. However, at 10 −3 M , IAA appeared to induce molecular dedifferentiation in that hypocotyl protein patterns began to resemble those of the hook. Histones also reflected differentiation, the hook having more histone types than the lower hypocotyl. IAA had no effect on histones. The hook region had two types of acidic chromosomal proteins, the lower hypocotyl one. When lower hypocotyl segments were incubated in 10 −3 M IAA, the protein pattern resembled that of the hook in that the second protein normally present in the hook and not in the hypocotyl was in fact induced in the hypocotyl. The hook had two acidic ribonuclear proteins, the lower hypocotyl one. IAA did not affect this protein. These experiments suggest that IAA in some manner regulates molecular (protein) differentiation. It is further suggested that IAA accomplishes this control through the acidic nuclear proteins which are closely associated with genetic material and which reflect differentiation and are also affected by IAA.
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Claims 11
Claim · model-written summary
Lower hypocotyl segments had more soluble protein types than hook regions.
Verbatim
e0“lower hypocotyl segments had more different protein types than did the hook region”
t0“hypocotyls of bean”
Claim · model-written summary
IAA at 10 −6 M appeared to induce more proteins in lower hypocotyl.
- Taxon
- beant0
- Organ / stage
- lower hypocotyle0
- Treatment
- Indoleacetic acid (IAA) at 10 −6 Me0
- Outcome
- different proteinse0
- Direction
- moree0
Not reportedComparator
Verbatim
e0“Indoleacetic acid (IAA) at 10 −6 M when applied to the lower hypocotyl appeared to induce still more different proteins”
t0“hypocotyls of bean”
Claim · model-written summary
IAA at 10 −3 M appeared to make hypocotyl protein patterns resemble the hook.
- Taxon
- beant0
- Treatment
- 10 −3 M , IAAe0
- Outcome
- hypocotyl protein patterns began to resemble those of the hooke0
- Comparator
- hooke0
Not reportedDirection
Verbatim
e0“at 10 −3 M , IAA appeared to induce molecular dedifferentiation in that hypocotyl protein patterns began to resemble those of the hook”
t0“hypocotyls of bean”
Claim · model-written summary
The hook had more histone types than lower hypocotyl.
Verbatim
e0“the hook having more histone types than the lower hypocotyl”
t0“hypocotyls of bean”
Claim · model-written summary
IAA had no effect on histones.
Not reportedComparator
Verbatim
e0“IAA had no effect on histones”
t0“hypocotyls of bean”
Claim · model-written summary
The hook had two acidic chromosomal protein types versus one in lower hypocotyl.
Verbatim
e0“The hook region had two types of acidic chromosomal proteins, the lower hypocotyl one”
t0“hypocotyls of bean”
Claim · model-written summary
IAA at 10 −3 M induced the second hook-associated acidic chromosomal protein in lower hypocotyl.
Verbatim
e0“When lower hypocotyl segments were incubated in 10 −3 M IAA, the protein pattern resembled that of the hook in that the second protein normally present in the hook and not in the hypocotyl was in fact induced in the hypocotyl”
t0“hypocotyls of bean”
Claim · model-written summary
The hook had two acidic ribonuclear proteins and lower hypocotyl one.
Verbatim
e0“The hook had two acidic ribonuclear proteins, the lower hypocotyl one”
t0“hypocotyls of bean”
Claim · model-written summary
IAA did not affect the acidic ribonuclear protein.
Not reportedComparator
Verbatim
e0“IAA did not affect this protein”
t0“hypocotyls of bean”
Claim · model-written summary
The authors suggest IAA regulates molecular protein differentiation.
Verbatim
e0“These experiments suggest that IAA in some manner regulates molecular (protein) differentiation”
t0“hypocotyls of bean”
Claim · model-written summary
The authors suggest acidic nuclear proteins mediate IAA control of differentiation.
Verbatim
e0“It is further suggested that IAA accomplishes this control through the acidic nuclear proteins”
t0“hypocotyls of bean”
The same questions in other papers
indole-3-acetic acid · molecular: metabolites · Phaseolus vulgaris (common bean)Positive and negative results
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See all 12 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.