Abstract
Soybean (Glycine max) RPG1-B (for resistance to Pseudomonas syringae pv glycinea) mediates species-specific resistance to P. syringae expressing the avirulence protein AvrB, similar to the nonorthologous RPM1 in Arabidopsis (Arabidopsis thaliana). RPM1-derived signaling is presumably induced upon AvrB-derived modification of the RPM1-interacting protein, RIN4 (for RPM1-interacting 4). We show that, similar to RPM1, RPG1-B does not directly interact with AvrB but associates with RIN4-like proteins from soybean. Unlike Arabidopsis, soybean contains at least four RIN4-like proteins (GmRIN4a to GmRIN4d). GmRIN4b, but not GmRIN4a, complements the Arabidopsis rin4 mutation. Both GmRIN4a and GmRIN4b bind AvrB, but only GmRIN4b binds RPG1-B. Silencing either GmRIN4a or GmRIN4b abrogates RPG1-B-derived resistance to P. syringae expressing AvrB. Binding studies show that GmRIN4b interacts with GmRIN4a as well as with two other AvrB/RPG1-B-interacting isoforms, GmRIN4c and GmRIN4d. The lack of functional redundancy among GmRIN4a and GmRIN4b and their abilities to interact with each other suggest that the two proteins might function as a heteromeric complex in mediating RPG1-B-derived resistance. Silencing GmRIN4a or GmRIN4b in rpg1-b plants enhances basal resistance to virulent strains of P. syringae and the oomycete Phytophthora sojae. Interestingly, GmRIN4a- or GmRIN4b-silenced rpg1-b plants respond differently to AvrB-expressing bacteria. Although both GmRIN4a and GmRIN4b function to monitor AvrB in the presence of RPG1-B, GmRIN4a, but not GmRIN4b, negatively regulates AvrB virulence activity in the absence of RPG1-B.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 13
Claim · model-written summary
RPG1-B associated with soybean RIN4-like proteins but did not directly bind AvrB.
- Taxon
- soybean
Glycine maxe0 - Gene
- RPG1-Be0
- Outcome
- associates with RIN4-like proteinse0
- Qualifier
- does not directly interact with AvrBe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RPG1-B does not directly interact with AvrB but associates with RIN4-like proteins from soybean.”
Claim · model-written summary
Soybean contained at least four RIN4-like proteins.
- Taxon
- soybeanGlycine maxe0
- Gene
- RIN4-like proteinse0
- Outcome
- at least four RIN4-like proteinse0
- Value
- at least foure0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“soybean contains at least four RIN4-like proteins (GmRIN4a to GmRIN4d).”
Claim · model-written summary
GmRIN4b complemented the Arabidopsis rin4 mutation.
- Taxon
- Arabidopsis
Arabidopsise0 - Treatment
- GmRIN4be0
- Gene
- GmRIN4be0
- Outcome
- complements the Arabidopsis rin4 mutatione0
- Comparator
- GmRIN4ae0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GmRIN4b, but not GmRIN4a, complements the Arabidopsis rin4 mutation.”
Claim · model-written summary
GmRIN4a did not complement the Arabidopsis rin4 mutation.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- GmRIN4ae0
- Gene
- GmRIN4ae0
- Outcome
- not GmRIN4a, complements the Arabidopsis rin4 mutatione0
- Comparator
- GmRIN4be0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GmRIN4b, but not GmRIN4a, complements the Arabidopsis rin4 mutation.”
Claim · model-written summary
GmRIN4a and GmRIN4b both bound AvrB.
- Taxon
- soybeanGlycine maxt0
- Gene
- GmRIN4a and GmRIN4be0
- Outcome
- bind AvrBe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Both GmRIN4a and GmRIN4b bind AvrB, but only GmRIN4b binds RPG1-B.”
t0“Soybean”
Claim · model-written summary
Only GmRIN4b bound RPG1-B.
- Taxon
- soybeanGlycine maxt0
- Gene
- GmRIN4be0
- Outcome
- binds RPG1-Be0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Both GmRIN4a and GmRIN4b bind AvrB, but only GmRIN4b binds RPG1-B.”
t0“Soybean”
Claim · model-written summary
Silencing GmRIN4a abolished RPG1-B-derived resistance to P. syringae expressing AvrB.
- Taxon
- soybeanGlycine maxt0
- Treatment
- Silencing either GmRIN4a or GmRIN4be0
- Interactor
- P. syringaee0pathogen
- Gene
- GmRIN4ae0
- Outcome
- RPG1-B-derived resistancee0
- Direction
- abrogatese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing either GmRIN4a or GmRIN4b abrogates RPG1-B-derived resistance to P. syringae expressing AvrB.”
t0“Soybean”
Claim · model-written summary
Silencing GmRIN4b abolished RPG1-B-derived resistance to P. syringae expressing AvrB.
- Taxon
- soybeanGlycine maxt0
- Treatment
- Silencing either GmRIN4a or GmRIN4be0
- Interactor
- P. syringaee0pathogen
- Gene
- GmRIN4be0
- Outcome
- RPG1-B-derived resistancee0
- Direction
- abrogatese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing either GmRIN4a or GmRIN4b abrogates RPG1-B-derived resistance to P. syringae expressing AvrB.”
t0“Soybean”
Claim · model-written summary
GmRIN4b interacted with GmRIN4a, GmRIN4c and GmRIN4d.
- Taxon
- soybeanGlycine maxt0
- Gene
- GmRIN4be0
- Outcome
- interacts with GmRIN4a as well as with two other AvrB/RPG1-B-interacting isoforms, GmRIN4c and GmRIN4de0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GmRIN4b interacts with GmRIN4a as well as with two other AvrB/RPG1-B-interacting isoforms, GmRIN4c and GmRIN4d.”
t0“Soybean”
Claim · model-written summary
The authors suggest GmRIN4a and GmRIN4b may form a heteromeric resistance complex.
- Taxon
- soybeanGlycine maxt0
- Outcome
- RPG1-B-derived resistancee0
- Qualifier
- might function as a heteromeric complexe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the two proteins might function as a heteromeric complex in mediating RPG1-B-derived resistance.”
t0“Soybean”
Claim · model-written summary
Silencing GmRIN4a or GmRIN4b in rpg1-b plants enhanced basal resistance to P. syringae.
- Taxon
- soybeanGlycine maxt0
- Treatment
- Silencing GmRIN4a or GmRIN4be0
- Interactor
- P. syringaee0pathogen
- Outcome
- basal resistance to virulent strains of P. syringaee0
- Direction
- enhancese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing GmRIN4a or GmRIN4b in rpg1-b plants enhances basal resistance to virulent strains of P. syringae and the oomycete Phytophthora sojae.”
t0“Soybean”
Claim · model-written summary
Silencing GmRIN4a or GmRIN4b in rpg1-b plants enhanced basal resistance to Phytophthora sojae.
- Taxon
- soybeanGlycine maxt0
- Treatment
- Silencing GmRIN4a or GmRIN4be0
- Interactor
- Phytophthora sojaePhytophthora sojaee0pathogen
- Outcome
- basal resistance to virulent strains of P. syringae and the oomycete Phytophthora sojaee0
- Direction
- enhancese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing GmRIN4a or GmRIN4b in rpg1-b plants enhances basal resistance to virulent strains of P. syringae and the oomycete Phytophthora sojae.”
t0“Soybean”
Claim · model-written summary
GmRIN4a but not GmRIN4b negatively regulated AvrB virulence without RPG1-B.
- Taxon
- soybeanGlycine maxt0
- Gene
- GmRIN4ae0
- Outcome
- AvrB virulence activitye0
- Direction
- negatively regulatese0
- Qualifier
- in the absence of RPG1-Be0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GmRIN4a, but not GmRIN4b, negatively regulates AvrB virulence activity in the absence of RPG1-B.”
t0“Soybean”
The same questions in other papers
loss of function · Phytophthora sojae · Glycine max (soybean)Positive and negative results
8 papers in the same direction as this paper · 15 in the opposite direction
- negative
- negative
- negative
20 papers more in this group
See all 24 papers →loss of function · disease · Glycine max (soybean)Positive and negative results
16 papers in the same direction as this paper · 20 in the opposite direction
- negative
- negative
- negative
33 papers more in this group
See all 37 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.