Abstract
Soybean mosaic virus (SMV) is one of the most destructive viral diseases in soybeans (Glycine max). In this study, an interaction between the SMV P3 protein and cytochrome b5 was detected by yeast two-hybrid assay, and bimolecular fluorescence complementation assay showed that the interaction took place at the cell periphery. Further, the interaction was confirmed by co-immunoprecipitation analysis. Quantitative real-time polymerase chain reaction analysis revealed that GmCYB5 gene was differentially expressed in resistant and susceptible soybean plants after inoculation with SMV-SC15 strain. To test the involvement of this gene in SMV resistance, the GmCYB5 was silenced using a bean pod mottle virus (BPMV)-based vector construct. Results showed that GmCYB5-1 was 83% and 99% downregulated in susceptible (NN1138-2) and resistant (RN-9) cultivars, respectively, compared to the empty vector-treated plants. Silencing of GmCYB5 gene promotes SMV replication in soybean plants. Our results suggest that during SMV infection, the host CYB5 protein targets P3 protein to inhibit its proliferation. Taken together, these results suggest that CYB5 is an important factor in SMV infection and replication in soybeans, which could help soybean breeders develop SMV resistant soybean cultivars.
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Claims 7
Claim · model-written summary
SMV P3 protein interacted with cytochrome b5.
- Taxon
- soybeans
Glycine maxe0 - Interactor
- SMVe1pathogen
- Gene
- cytochrome b5e1
- Outcome
- interaction between the SMV P3 protein and cytochrome b5e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“soybeans (Glycine max)”
e1“an interaction between the SMV P3 protein and cytochrome b5 was detected by yeast two-hybrid assay”
Claim · model-written summary
The P3–cytochrome b5 interaction occurred at the cell periphery.
- Taxon
- soybeansGlycine maxe0
- Interactor
- SMVe2pathogen
- Gene
- cytochrome b5e2
- Outcome
- interaction took place at the cell peripherye1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“soybeans (Glycine max)”
e1“bimolecular fluorescence complementation assay showed that the interaction took place at the cell periphery”
e2“SMV P3 protein and cytochrome b5”
Claim · model-written summary
GmCYB5 expression differed between resistant and susceptible soybean after SMV-SC15 inoculation.
- Taxon
- soybeanGlycine maxe0
- Material
- resistant and susceptible soybean plantse0
- Interactor
- SMV-SC15e0pathogen
- Gene
- GmCYB5e0
- Outcome
- differentially expressede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GmCYB5 gene was differentially expressed in resistant and susceptible soybean plants after inoculation with SMV-SC15 strain”
Claim · model-written summary
Silencing GmCYB5-1 downregulated it by 83% in susceptible NN1138-2.
- Taxon
- soybeanGlycine maxe0
- Material
- NN1138-2e1
- Treatment
- downregulatede1
- Gene
- GmCYB5-1e1
- Outcome
- GmCYB5-1e1
- Direction
- downregulatede1
- Value
- 83%e1
- Comparator
- empty vector-treated plantse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“replication in soybean plants”
e1“GmCYB5-1 was 83% and 99% downregulated in susceptible (NN1138-2) and resistant (RN-9) cultivars, respectively, compared to the empty vector-treated plants”
Claim · model-written summary
Silencing GmCYB5-1 downregulated it by 99% in resistant RN-9.
- Taxon
- soybeanGlycine maxe0
- Material
- RN-9e1
- Treatment
- downregulatede1
- Gene
- GmCYB5-1e1
- Outcome
- GmCYB5-1e1
- Direction
- downregulatede1
- Value
- 99%e1
- Comparator
- empty vector-treated plantse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“replication in soybean plants”
e1“GmCYB5-1 was 83% and 99% downregulated in susceptible (NN1138-2) and resistant (RN-9) cultivars, respectively, compared to the empty vector-treated plants”
Claim · model-written summary
GmCYB5 silencing promoted SMV replication in soybean.
- Taxon
- soybeanGlycine maxe0
- Treatment
- Silencing of GmCYB5 genee0
- Interactor
- SMVe0pathogen
- Gene
- GmCYB5e0
- Outcome
- SMV replicatione0
- Direction
- promotese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing of GmCYB5 gene promotes SMV replication in soybean plants.”
Claim · model-written summary
The authors infer that soybean CYB5 targets viral P3 to inhibit its proliferation.
- Taxon
- soybeanGlycine maxe1
- Interactor
- SMVe0pathogen
- Gene
- CYB5 proteine0
- Outcome
- inhibit its proliferatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“during SMV infection, the host CYB5 protein targets P3 protein to inhibit its proliferation”
e1“replication in soybean plants”
The same questions in other papers
loss of function · disease · Glycine max (soybean)Positive and negative results
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- positive
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