Abstract
The biochemical function of the potyviral P3 protein is not known, although it is known to regulate virus replication, movement, and pathogenesis. We show that P3, the putative virulence determinant of soybean mosaic virus (SMV), targets a component of the translation elongation complex in soybean. Eukaryotic elongation factor 1A (eEF1A), a well-known host factor in viral pathogenesis, is essential for SMV virulence and the associated unfolded protein response (UPR). Silencing GmEF1A inhibits accumulation of SMV and another ER-associated virus in soybean. Conversely, endoplasmic reticulum (ER) stress-inducing chemicals promote SMV accumulation in wild-type, but not GmEF1A-knockdown, plants. Knockdown of genes encoding the eEF1B isoform, which is important for eEF1A function in translation elongation, has similar effects on UPR and SMV resistance, suggesting a link to translation elongation. P3 and GmEF1A promote each other's nuclear localization, similar to the nuclear-cytoplasmic transport of eEF1A by the Human immunodeficiency virus 1 Nef protein. Our results suggest that P3 targets host elongation factors resulting in UPR, which in turn facilitates SMV replication and place eEF1A upstream of BiP in the ER stress response during pathogen infection.
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Claims 10
Claim · model-written summary
SMV P3 targets a translation elongation complex component in soybean.
- Taxon
- soybean
Glycine maxe0 - Interactor
- soybean mosaic virus (SMV)
Soybean mosaic viruse0pathogen - Gene
- P3e0
- Outcome
- targets a component of the translation elongation complexe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“P3, the putative virulence determinant of soybean mosaic virus (SMV), targets a component of the translation elongation complex in soybean”
Claim · model-written summary
eEF1A is essential for SMV virulence and the associated UPR.
- Taxon
- soybeanGlycine maxe1
- Interactor
- SMVe0pathogen
- Gene
- Eukaryotic elongation factor 1A (eEF1A)e0
- Outcome
- SMV virulence and the associated unfolded protein response (UPR)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Eukaryotic elongation factor 1A (eEF1A), a well-known host factor in viral pathogenesis, is essential for SMV virulence and the associated unfolded protein response (UPR)”
t0“Potyviral”
e1“P3, the putative virulence determinant of soybean mosaic virus (SMV), targets a component of the translation elongation complex in soybean”
Claim · model-written summary
GmEF1A silencing inhibits SMV accumulation.
- Taxon
- soybeanGlycine maxe0
- Treatment
- Silencing GmEF1Ae0
- Interactor
- SMVe0pathogen
- Gene
- GmEF1Ae0
- Outcome
- accumulation of SMVe0
- Direction
- inhibitse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing GmEF1A inhibits accumulation of SMV and another ER-associated virus in soybean”
Claim · model-written summary
GmEF1A silencing also inhibits another ER-associated virus.
- Taxon
- soybeanGlycine maxe0
- Treatment
- Silencing GmEF1Ae0
- Interactor
- another ER-associated viruse0pathogen
- Outcome
- another ER-associated viruse0
- Direction
- inhibitse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing GmEF1A inhibits accumulation of SMV and another ER-associated virus in soybean”
Claim · model-written summary
ER stress chemicals promote SMV accumulation in wild-type plants.
- Taxon
- soybeanGlycine maxe1
- Treatment
- endoplasmic reticulum (ER) stress-inducing chemicalse0
- Interactor
- SMVe0pathogen
- Outcome
- SMV accumulatione0
- Direction
- promotee0
- Comparator
- GmEF1A-knockdowne0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“endoplasmic reticulum (ER) stress-inducing chemicals promote SMV accumulation in wild-type, but not GmEF1A-knockdown, plants”
t0“Potyviral”
e1“P3, the putative virulence determinant of soybean mosaic virus (SMV), targets a component of the translation elongation complex in soybean”
Claim · model-written summary
ER stress chemicals did not promote SMV accumulation in GmEF1A knockdown plants.
- Taxon
- soybeanGlycine maxe1
- Treatment
- endoplasmic reticulum (ER) stress-inducing chemicalse0
- Interactor
- SMVe0pathogen
- Outcome
- SMV accumulatione0
- Direction
- but note0
- Comparator
- wild-typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“endoplasmic reticulum (ER) stress-inducing chemicals promote SMV accumulation in wild-type, but not GmEF1A-knockdown, plants”
t0“Potyviral”
e1“P3, the putative virulence determinant of soybean mosaic virus (SMV), targets a component of the translation elongation complex in soybean”
Claim · model-written summary
eEF1B knockdown similarly affected UPR and SMV resistance.
- Taxon
- soybeanGlycine maxe1
- Treatment
- Knockdown of genes encoding the eEF1B isoforme0
- Interactor
- SMVe0pathogen
- Gene
- eEF1Be0
- Outcome
- UPR and SMV resistancee0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Knockdown of genes encoding the eEF1B isoform, which is important for eEF1A function in translation elongation, has similar effects on UPR and SMV resistance”
t0“Potyviral”
e1“P3, the putative virulence determinant of soybean mosaic virus (SMV), targets a component of the translation elongation complex in soybean”
Claim · model-written summary
P3 and GmEF1A promote each other’s nuclear localization.
- Taxon
- soybeanGlycine maxe1
- Gene
- GmEF1Ae0
- Outcome
- nuclear localizatione0
- Direction
- promotee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“P3 and GmEF1A promote each other's nuclear localization”
t0“Potyviral”
e1“P3, the putative virulence determinant of soybean mosaic virus (SMV), targets a component of the translation elongation complex in soybean”
Claim · model-written summary
The authors infer that P3 targets host elongation factors to induce UPR and aid SMV replication.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“P3 targets host elongation factors resulting in UPR, which in turn facilitates SMV replication”
t0“Potyviral”
e1“P3, the putative virulence determinant of soybean mosaic virus (SMV), targets a component of the translation elongation complex in soybean”
Claim · model-written summary
eEF1A was placed upstream of BiP in the infection-associated ER stress response.
- Taxon
- soybeanGlycine maxe1
- Gene
- eEF1Ae0
- Outcome
- ER stress responsee0
- Qualifier
- during pathogen infectione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“place eEF1A upstream of BiP in the ER stress response during pathogen infection”
t0“Potyviral”
e1“P3, the putative virulence determinant of soybean mosaic virus (SMV), targets a component of the translation elongation complex in soybean”
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loss of function · disease · Glycine max (soybean)Positive and negative results
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