Abstract
Cold temperatures trigger the expression of the CBF family of transcription factors, which in turn activate many downstream genes that confer chilling and freezing tolerance to plants. We report here the identification of ICE1 (inducer of CBF expression 1), an upstream transcription factor that regulates the transcription of CBF genes in the cold. An Arabidopsis ice1 mutant was isolated in a screen for mutations that impair cold-induced transcription of a CBF3 promoter-luciferase reporter gene. The ice1 mutation blocks the expression of CBF3 and decreases the expression of many genes downstream of CBFs, which leads to a significant reduction in plant chilling and freezing tolerance. ICE1 encodes a MYC-like bHLH transcriptional activator. ICE1 binds specifically to the MYC recognition sequences in the CBF3 promoter. ICE1 is expressed constitutively, and its overexpression in wild-type plants enhances the expression of the CBF regulon in the cold and improves freezing tolerance of the transgenic plants.
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Claims 10
Claim · model-written summary
ICE1 was identified as an upstream regulator of cold-induced CBF transcription.
- Taxon
- Arabidopsis
Arabidopsist0 - Gene
- ICE1e0
- Outcome
- transcription of CBF genese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We report here the identification of ICE1 (inducer of CBF expression 1), an upstream transcription factor that regulates the transcription of CBF genes in the cold.”
t0“Arabidopsis”
Claim · model-written summary
An Arabidopsis ice1 mutant was isolated by impaired cold-induced CBF3 reporter transcription.
- Taxon
- ArabidopsisArabidopsise0
- Material
- ice1 mutante0
- Gene
- CBF3e0
- Outcome
- mutations that impair cold-induced transcription of a CBF3 promoter-luciferase reporter genee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“An Arabidopsis ice1 mutant was isolated in a screen for mutations that impair cold-induced transcription of a CBF3 promoter-luciferase reporter gene.”
Claim · model-written summary
The ice1 mutation blocked CBF3 expression.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- ice1 mutatione0
- Gene
- CBF3e0
- Outcome
- expression of CBF3e0
- Direction
- blockse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The ice1 mutation blocks the expression of CBF3 and decreases the expression of many genes downstream of CBFs, which leads to a significant reduction in plant chilling and freezing tolerance.”
t0“Arabidopsis”
Claim · model-written summary
The ice1 mutation decreased expression of many genes downstream of CBFs.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- ice1 mutatione0
- Outcome
- expression of many genes downstream of CBFse0
- Direction
- decreasese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The ice1 mutation blocks the expression of CBF3 and decreases the expression of many genes downstream of CBFs, which leads to a significant reduction in plant chilling and freezing tolerance.”
t0“Arabidopsis”
Claim · model-written summary
The ice1 mutation significantly reduced chilling and freezing tolerance.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- ice1 mutatione0
- Outcome
- plant chilling and freezing tolerancee0
- Direction
- reductione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The ice1 mutation blocks the expression of CBF3 and decreases the expression of many genes downstream of CBFs, which leads to a significant reduction in plant chilling and freezing tolerance.”
t0“Arabidopsis”
Claim · model-written summary
ICE1 encodes a MYC-like bHLH transcriptional activator.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- ICE1e0
- Outcome
- MYC-like bHLH transcriptional activatore0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ICE1 encodes a MYC-like bHLH transcriptional activator.”
t0“Arabidopsis”
Claim · model-written summary
ICE1 specifically binds MYC recognition sequences in the CBF3 promoter.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- ICE1e0
- Outcome
- binds specifically to the MYC recognition sequences in the CBF3 promotere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ICE1 binds specifically to the MYC recognition sequences in the CBF3 promoter.”
t0“Arabidopsis”
Claim · model-written summary
ICE1 was expressed constitutively.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- ICE1e0
- Outcome
- expressed constitutivelye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ICE1 is expressed constitutively, and its overexpression in wild-type plants enhances the expression of the CBF regulon in the cold and improves freezing tolerance of the transgenic plants.”
t0“Arabidopsis”
Claim · model-written summary
ICE1 overexpression increased expression of the CBF regulon in cold conditions.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- overexpression in wild-type plantse0
- Gene
- ICE1e0
- Outcome
- expression of the CBF regulone0
- Direction
- enhancese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ICE1 is expressed constitutively, and its overexpression in wild-type plants enhances the expression of the CBF regulon in the cold and improves freezing tolerance of the transgenic plants.”
t0“Arabidopsis”
Claim · model-written summary
ICE1 overexpression improved freezing tolerance of transgenic plants.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- overexpression in wild-type plantse0
- Gene
- ICE1e0
- Outcome
- freezing tolerance of the transgenic plantse0
- Direction
- improvese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ICE1 is expressed constitutively, and its overexpression in wild-type plants enhances the expression of the CBF regulon in the cold and improves freezing tolerance of the transgenic plants.”
t0“Arabidopsis”
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