Abstract
F-box proteins play diverse roles in regulating numerous physiological processes in plants. This study isolated a gene (OsFbx352) from rice encoding an F-box domain protein and characterized its role in seed germination. Expression of OsFbx352 was upregulated by abscisic acid (ABA). The transcripts of OsFbx352 were increased upon imbibition of rice seeds and the increase was markedly suppressed by glucose. Germination of seeds with overexpression of OsFbx352 was less suppressed by glucose than that of wild-type seeds, while glucose had greater inhibition for germination of seeds with knockdown of OsFbx352 by RNA interference (RNAi) than that of wild-type seeds. The differential response of germination of the transgenic and wild-type seeds to glucose may be accounted for by differences in ABA content among overexpressing, RNAi, and wild-type seeds such that overexpression of OsFbx352 and knockdown of OsFbx352 led to lower and higher ABA contents, respectively, than that of wild-type seeds in the presence of glucose. Overexpression of OsFbx352 led to a reduction in expression of genes responsible for ABA synthesis (OsNced2, OsNced3) and an increase in expression of genes encoding ABA catabolism (OsAba-ox2, OsAba-ox3) in the presence of glucose. These findings indicate that OsFbx352 plays a regulatory role in the regulation of glucose-induced suppression of seed germination by targeting ABA metabolism.
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Claims 11
Claim · model-written summary
The study isolated OsFbx352 from rice as an F-box domain protein gene.
- Taxon
- rice
Oryza sativae0 - Gene
- OsFbx352e0
- Outcome
- isolated a gene (OsFbx352)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This study isolated a gene (OsFbx352) from rice encoding an F-box domain protein”
Claim · model-written summary
ABA upregulated OsFbx352 expression.
- Taxon
- riceOryza sativae1
- Treatment
- abscisic acid (ABA)e0
- Gene
- OsFbx352e0
- Outcome
- Expression of OsFbx352e0
- Direction
- upregulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of OsFbx352 was upregulated by abscisic acid (ABA)”
e1“from rice”
Claim · model-written summary
OsFbx352 transcripts increased upon rice seed imbibition.
- Taxon
- riceOryza sativae0
- Organ / stage
- seedse0
- Gene
- OsFbx352e0
- Outcome
- transcripts of OsFbx352e0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The transcripts of OsFbx352 were increased upon imbibition of rice seeds”
Claim · model-written summary
Glucose markedly suppressed the imbibition-induced increase in OsFbx352 transcripts.
- Taxon
- riceOryza sativae1
- Treatment
- glucosee0
- Gene
- OsFbx352e1
- Outcome
- transcripts of OsFbx352e1
- Direction
- suppressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the increase was markedly suppressed by glucose”
e1“The transcripts of OsFbx352 were increased upon imbibition of rice seeds”
Claim · model-written summary
Glucose suppressed germination less in OsFbx352 overexpression seeds than wild-type seeds.
- Taxon
- riceOryza sativae1
- Treatment
- overexpression of OsFbx352e0
- Gene
- OsFbx352e0
- Outcome
- Germinatione0
- Direction
- less suppressede0
- Comparator
- wild-type seedse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Germination of seeds with overexpression of OsFbx352 was less suppressed by glucose than that of wild-type seeds”
e1“from rice”
Claim · model-written summary
Glucose inhibited germination more in OsFbx352 RNAi seeds than wild-type seeds.
- Taxon
- riceOryza sativae1
- Treatment
- knockdown of OsFbx352 by RNA interference (RNAi)e0
- Gene
- OsFbx352e0
- Outcome
- germinatione0
- Direction
- greater inhibitione0
- Comparator
- wild-type seedse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“glucose had greater inhibition for germination of seeds with knockdown of OsFbx352 by RNA interference (RNAi) than that of wild-type seeds”
e1“from rice”
Claim · model-written summary
OsFbx352 overexpression lowered ABA content relative to wild type under glucose.
- Taxon
- riceOryza sativae1
- Treatment
- overexpression of OsFbx352e0
- Outcome
- ABA contentse0
- Direction
- lowere0
- Qualifier
- in the presence of glucosee0
- Comparator
- wild-type seedse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression of OsFbx352 and knockdown of OsFbx352 led to lower and higher ABA contents, respectively, than that of wild-type seeds in the presence of glucose”
e1“from rice”
Claim · model-written summary
OsFbx352 knockdown raised ABA content relative to wild type under glucose.
- Taxon
- riceOryza sativae1
- Treatment
- knockdown of OsFbx352e0
- Outcome
- ABA contentse0
- Direction
- highere0
- Qualifier
- in the presence of glucosee0
- Comparator
- wild-type seedse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression of OsFbx352 and knockdown of OsFbx352 led to lower and higher ABA contents, respectively, than that of wild-type seeds in the presence of glucose”
e1“from rice”
Claim · model-written summary
OsFbx352 overexpression reduced expression of ABA synthesis genes OsNced2 and OsNced3 under glucose.
- Taxon
- riceOryza sativae1
- Treatment
- Overexpression of OsFbx352e0
- Gene
- OsNced2, OsNced3e0
- Outcome
- expression of genes responsible for ABA synthesise0
- Direction
- reductione0
- Qualifier
- in the presence of glucosee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of OsFbx352 led to a reduction in expression of genes responsible for ABA synthesis (OsNced2, OsNced3) and an increase in expression of genes encoding ABA catabolism (OsAba-ox2, OsAba-ox3) in the presence of glucose”
e1“from rice”
Claim · model-written summary
OsFbx352 overexpression increased expression of ABA catabolism genes OsAba-ox2 and OsAba-ox3 under glucose.
- Taxon
- riceOryza sativae1
- Treatment
- Overexpression of OsFbx352e0
- Gene
- OsAba-ox2, OsAba-ox3e0
- Outcome
- expression of genes encoding ABA catabolisme0
- Direction
- increasee0
- Qualifier
- in the presence of glucosee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of OsFbx352 led to a reduction in expression of genes responsible for ABA synthesis (OsNced2, OsNced3) and an increase in expression of genes encoding ABA catabolism (OsAba-ox2, OsAba-ox3) in the presence of glucose”
e1“from rice”
Claim · model-written summary
The authors infer OsFbx352 regulates glucose-induced germination suppression through ABA metabolism.
- Taxon
- riceOryza sativae1
- Gene
- OsFbx352e0
- Outcome
- glucose-induced suppression of seed germinatione0
- Qualifier
- targeting ABA metabolisme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsFbx352 plays a regulatory role in the regulation of glucose-induced suppression of seed germination by targeting ABA metabolism”
e1“from rice”
The same questions in other papers
glucose · molecular: expression · Oryza sativa (rice)Positive and negative results
2 papers in the same direction as this paper · 6 in the opposite direction
- no effect
- positive
- positive
5 papers more in this group
See all 9 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.