Abstract
In plants, sugars are required to sustain growth and regulate gene expression. A large set of genes are either up- or down-regulated by sugars; however, whether there is a common mechanism and signal transduction pathway for differential and coordinated sugar regulation remain unclear. In the present study, the rice (Oryza sativa cv Tainan 5) cell culture was used as a model system to address this question. Sucrose and glucose both played dual functions in gene regulation as exemplified by the up-regulation of growth-related genes and down-regulation of stress-related genes. Sugar coordinately but differentially activated or repressed gene expression, and nuclear run-on transcription and mRNA half-life analyses revealed regulation of both the transcription rate and mRNA stability. Although coordinately regulated by sugars, these growth- and stress-related genes were up-regulated or down-regulated through hexokinase-dependent and/or hexokinase-independent pathways. We also found that the sugar signal transduction pathway may overlap the glycolytic pathway for gene repression. alpha-Amylase and the stress-related genes identified in this study were coordinately expressed under sugar starvation, suggesting a convergence of the nutritional and environmental stress signal transduction pathways. Together, our studies provide a new insight into the complex signal transduction network and mechanisms of sugar regulation of growth and stress-related genes in plants.
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Claims 7
Claim · model-written summary
Sucrose and glucose up-regulated growth-related genes in rice cell culture
- Taxon
- Oryza sativa
Oryza sativae0 - Material
- Tainan 5e0
- Organ / stage
- cell culturee0
- Treatment
- Sucrose and glucosee1
- Outcome
- growth-related genese1
- Direction
- up-regulatione1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the rice (Oryza sativa cv Tainan 5) cell culture was used as a model system”
e1“Sucrose and glucose both played dual functions in gene regulation as exemplified by the up-regulation of growth-related genes and down-regulation of stress-related genes”
Claim · model-written summary
Sucrose and glucose down-regulated stress-related genes in rice cell culture
- Taxon
- Oryza sativaOryza sativae0
- Material
- Tainan 5e0
- Organ / stage
- cell culturee0
- Treatment
- Sucrose and glucosee1
- Outcome
- stress-related genese1
- Direction
- down-regulatione1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the rice (Oryza sativa cv Tainan 5) cell culture was used as a model system”
e1“Sucrose and glucose both played dual functions in gene regulation as exemplified by the up-regulation of growth-related genes and down-regulation of stress-related genes”
Claim · model-written summary
Sugar regulated both the transcription rate and mRNA stability of gene expression in rice cell culture
- Taxon
- Oryza sativaOryza sativae0
- Treatment
- Sugare2
- Outcome
- transcription rate and mRNA stabilitye2
- Direction
- regulatione2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the rice (Oryza sativa cv Tainan 5) cell culture was used as a model system”
e2“Sugar coordinately but differentially activated or repressed gene expression, and nuclear run-on transcription and mRNA half-life analyses revealed regulation of both the transcription rate and mRNA stability”
Claim · model-written summary
Growth- and stress-related genes were regulated through hexokinase-dependent and hexokinase-independent pathways
- Taxon
- Oryza sativaOryza sativae0
- Outcome
- growth- and stress-related genese3
- Qualifier
- hexokinase-dependent and/or hexokinase-independent pathwayse3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the rice (Oryza sativa cv Tainan 5) cell culture was used as a model system”
e3“Although coordinately regulated by sugars, these growth- and stress-related genes were up-regulated or down-regulated through hexokinase-dependent and/or hexokinase-independent pathways”
Claim · model-written summary
The sugar signal transduction pathway may overlap the glycolytic pathway for gene repression
- Taxon
- Oryza sativaOryza sativae0
- Outcome
- gene repressione4
- Qualifier
- the sugar signal transduction pathway may overlap the glycolytic pathwaye4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the rice (Oryza sativa cv Tainan 5) cell culture was used as a model system”
e4“the sugar signal transduction pathway may overlap the glycolytic pathway for gene repression”
Claim · model-written summary
alpha-Amylase and stress-related genes were coordinately expressed under sugar starvation
- Taxon
- Oryza sativaOryza sativae0
- Treatment
- sugar starvatione5
- Gene
- alpha-Amylasee5
- Outcome
- coordinately expressede5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the rice (Oryza sativa cv Tainan 5) cell culture was used as a model system”
e5“alpha-Amylase and the stress-related genes identified in this study were coordinately expressed under sugar starvation”
Claim · model-written summary
Sugar starvation response suggests convergence of nutritional and environmental stress signal transduction pathways
- Taxon
- Oryza sativaOryza sativae0
- Outcome
- convergencee6
- Qualifier
- nutritional and environmental stress signal transduction pathwayse6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the rice (Oryza sativa cv Tainan 5) cell culture was used as a model system”
e6“suggesting a convergence of the nutritional and environmental stress signal transduction pathways”
The same questions in other papers
glucose · molecular: expression · Oryza sativa (rice)Positive and negative results
5 papers in the same direction as this paper · 3 in the opposite direction
- no effect
- negative
- negative
5 papers more in this group
See all 9 papers →sucrose · molecular: expression · Oryza sativa (rice)Positive and negative results
13 papers in the same direction as this paper · 4 in the opposite direction
- positive
- positive
- no effect
14 papers more in this group
See all 18 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.