Abstract
The relationship between nitric oxide (NO) and salicylic acid (SA) was investigated in Arabidopsis thaliana. Here it is shown that SA is able to induce NO synthesis in a dose-dependent manner in Arabidopsis. NO production was detected by confocal microscopic analysis and spectrofluorometric assay in plant roots and cultured cells. To identify the metabolic pathways involved in SA-induced NO synthesis, genetic and pharmacological approaches were adopted. The analysis of the nia1,nia2 mutant showed that nitrate reductase activity was not required for SA-induced NO production. Experiments performed in the presence of a nitric oxide synthase (NOS) inhibitor suggested the involvement of NOS-like enzyme activity in this metabolic pathway. Moreover, the production of NO by SA treatment of Atnos1 mutant plants was strongly reduced compared with wild-type plants. Components of the SA signalling pathway giving rise to NO production were identified, and both calcium and casein kinase 2 (CK2) were demonstrated to be involved. Taken together, these results suggest that SA induces NO production at least in part through the activity of a NOS-like enzyme and that calcium and CK2 activity are essential components of the signalling cascade.
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Claims 6
Claim · model-written summary
Salicylic acid induced nitric oxide synthesis in a dose-dependent manner.
- Taxon
- Arabidopsis
Arabidopsise0 - Treatment
- SAe0
- Outcome
- NO synthesise0
- Direction
- inducee0
- Qualifier
- dose-dependent mannere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SA is able to induce NO synthesis in a dose-dependent manner in Arabidopsis”
Claim · model-written summary
Nitrate reductase activity was unnecessary for salicylic-acid-induced nitric oxide production.
- Taxon
- ArabidopsisArabidopsist0
- Material
- nia1,nia2 mutante0
- Gene
- nitrate reductasee0
- Outcome
- SA-induced NO productione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The analysis of the nia1,nia2 mutant showed that nitrate reductase activity was not required for SA-induced NO production”
t0“Arabidopsis”
Claim · model-written summary
NOS-like enzyme activity was implicated in the salicylic-acid-induced pathway.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- nitric oxide synthase (NOS) inhibitore0
- Gene
- NOS-like enzymee0
- Outcome
- involvement of NOS-like enzyme activitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Experiments performed in the presence of a nitric oxide synthase (NOS) inhibitor suggested the involvement of NOS-like enzyme activity in this metabolic pathway”
t0“Arabidopsis”
Claim · model-written summary
Atnos1 mutants produced less nitric oxide after salicylic acid treatment than wild-type plants.
- Taxon
- ArabidopsisArabidopsist0
- Material
- Atnos1 mutant plantse0
- Treatment
- SA treatmente0
- Outcome
- production of NOe0
- Direction
- strongly reducede0
- Comparator
- wild-type plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“production of NO by SA treatment of Atnos1 mutant plants was strongly reduced compared with wild-type plants”
t0“Arabidopsis”
Claim · model-written summary
Calcium was involved in salicylic-acid signaling that produces nitric oxide.
- Taxon
- ArabidopsisArabidopsist0
- Compound
- calciume0
- Outcome
- involvede0
- Qualifier
- calciume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“both calcium and casein kinase 2 (CK2) were demonstrated to be involved”
t0“Arabidopsis”
Claim · model-written summary
Casein kinase 2 was involved in salicylic-acid signaling that produces nitric oxide.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- casein kinase 2 (CK2)e0
- Outcome
- involvede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“both calcium and casein kinase 2 (CK2) were demonstrated to be involved”
t0“Arabidopsis”
The same questions in other papers
salicylic acid · molecular: metabolites · ArabidopsisResults differ
6 papers in the same direction as this paper · 7 in the opposite direction
- positive
- positive
- no effect
10 papers more in this group
See all 14 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.