Abstract
Specific disease resistance of Arabidopsis thaliana against the Hyaloperonospora parasitica isolate Hiks1 (HpHiks1) is mediated by RPP7. Although this disease resistance gene encodes a typical nucleotide binding site leucine-rich repeat (NB-LRR) disease resistance protein, its function is independent of the defense hormone salicylic acid and most known genes required for plant immune responses. We identified EDM2 (enhanced downy mildew 2) in a genetic screen for RPP7 suppressors. Mutations of EDM2 phenocopy RPP7 mutations, but do not affect other tested disease resistance genes. We isolated EDM2 by map-based cloning. The predicted EDM2 protein is structurally unrelated to previously identified components of the plant immune system, bears typical features of transcriptional regulators, including plant homeodomain (PHD)-finger-like domains, and defines a plant-specific protein family. In edm2 mutants both constitutive and HpHiks1-induced RPP7 transcript levels are reduced, suggesting that EDM2 is either a direct or an indirect regulator of RPP7 expression. Microarray analyses defined a set of defense-associated genes, the expression of which is suppressed during successful HpHiks1 colonization of either rpp7 or edm2 plants. This transcriptional phenotype is counteracted by an EDM2/RPP7-dependent mechanism.
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Claims 9
Claim · model-written summary
Mutations of EDM2 phenocopy RPP7 mutations in Arabidopsis.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae0 - Treatment
- Mutations of EDM2e1
- Outcome
- phenocopye1
- Comparator
- RPP7 mutationse1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Specific disease resistance of Arabidopsis thaliana against the Hyaloperonospora parasitica isolate Hiks1 (HpHiks1) is mediated by RPP7”
e1“Mutations of EDM2 phenocopy RPP7 mutations, but do not affect other tested disease resistance genes”
Claim · model-written summary
EDM2 mutations do not affect other tested disease resistance genes.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- Mutations of EDM2e1
- Outcome
- other tested disease resistance genese1
- Direction
- do not affecte1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Specific disease resistance of Arabidopsis thaliana against the Hyaloperonospora parasitica isolate Hiks1 (HpHiks1) is mediated by RPP7”
e1“Mutations of EDM2 phenocopy RPP7 mutations, but do not affect other tested disease resistance genes”
Claim · model-written summary
EDM2 was identified in a genetic screen for RPP7 suppressors.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Gene
- EDM2e2
- Outcome
- RPP7 suppressorse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Specific disease resistance of Arabidopsis thaliana against the Hyaloperonospora parasitica isolate Hiks1 (HpHiks1) is mediated by RPP7”
e2“We identified EDM2 (enhanced downy mildew 2) in a genetic screen for RPP7 suppressors”
Claim · model-written summary
The predicted EDM2 protein has plant homeodomain (PHD)-finger-like domains.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Gene
- EDM2e3
- Outcome
- plant homeodomain (PHD)-finger-like domainse3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Specific disease resistance of Arabidopsis thaliana against the Hyaloperonospora parasitica isolate Hiks1 (HpHiks1) is mediated by RPP7”
e3“The predicted EDM2 protein is structurally unrelated to previously identified components of the plant immune system, bears typical features of transcriptional regulators, including plant homeodomain (PHD)-finger-like domains, and defines a plant-specific protein family”
Claim · model-written summary
EDM2 defines a plant-specific protein family.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Gene
- EDM2e3
- Outcome
- plant-specific protein familye3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Specific disease resistance of Arabidopsis thaliana against the Hyaloperonospora parasitica isolate Hiks1 (HpHiks1) is mediated by RPP7”
e3“The predicted EDM2 protein is structurally unrelated to previously identified components of the plant immune system, bears typical features of transcriptional regulators, including plant homeodomain (PHD)-finger-like domains, and defines a plant-specific protein family”
Claim · model-written summary
In edm2 mutants, constitutive and HpHiks1-induced RPP7 transcript levels are reduced.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- edm2 mutantse4
- Gene
- RPP7e4
- Outcome
- RPP7 transcript levelse4
- Direction
- reducede4
- Qualifier
- both constitutive and HpHiks1-inducede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Specific disease resistance of Arabidopsis thaliana against the Hyaloperonospora parasitica isolate Hiks1 (HpHiks1) is mediated by RPP7”
e4“In edm2 mutants both constitutive and HpHiks1-induced RPP7 transcript levels are reduced”
Claim · model-written summary
EDM2 is a direct or indirect regulator of RPP7 expression (author suggestion).
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Gene
- EDM2e5
- Outcome
- RPP7 expressione5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Specific disease resistance of Arabidopsis thaliana against the Hyaloperonospora parasitica isolate Hiks1 (HpHiks1) is mediated by RPP7”
e5“suggesting that EDM2 is either a direct or an indirect regulator of RPP7 expression”
Claim · model-written summary
Defense-associated genes were suppressed during successful HpHiks1 colonization of rpp7 or edm2 plants.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Material
- rpp7 or edm2 plantse6
- Treatment
- successful HpHiks1 colonizatione6
- Interactor
- HpHiks1e6pathogen
- Outcome
- defense-associated genese6
- Direction
- suppressede6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Specific disease resistance of Arabidopsis thaliana against the Hyaloperonospora parasitica isolate Hiks1 (HpHiks1) is mediated by RPP7”
e6“Microarray analyses defined a set of defense-associated genes, the expression of which is suppressed during successful HpHiks1 colonization of either rpp7 or edm2 plants”
Claim · model-written summary
The suppressed transcriptional phenotype is counteracted by an EDM2/RPP7-dependent mechanism (author interpretation).
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Gene
- EDM2/RPP7e7
- Outcome
- transcriptional phenotypee7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Specific disease resistance of Arabidopsis thaliana against the Hyaloperonospora parasitica isolate Hiks1 (HpHiks1) is mediated by RPP7”
e7“This transcriptional phenotype is counteracted by an EDM2/RPP7-dependent mechanism”
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