Abstract
OsSUT1 encodes a rice sucrose transport protein that is highly expressed in developing grain, leaf sheath and stem after heading, and in germinating seedlings, but only at very low levels in source leaves. In this study, we have used antisense gene suppression to elucidate the in vivo function of OsSUT1. Rice was transformed with an antisense construct containing a portion of the 3'-coding and non-coding regions of OsSUT1 driven by the maize ubiquitin-1 promoter. Twenty-six independent stably transformed lines were obtained. T0 and selfed T1 progeny were analysed for suppression of OsSUT1 expression and function. Many of the plants showed a significant reduction in their ability to produce filled grain, and final grain weight was reduced. Severe phenotypes correlated with a reduction in OsSUT1 transcript level in filling grain. Unlike SUT1 antisense suppression in dicots, source supply of photosynthate was unaffected in these transformants. This provides the first direct evidence for the requirement of a sucrose transporter for grain filling in a cereal species. Furthermore, seed from some of the T0 population showed a reduction in the rate of germination and growth, supporting the hypothesis that OsSUT1 may also play a role in transporting sucrose remobilized from starch reserves in germinating seeds.
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Claims 7
Claim · model-written summary
OsSUT1 expression was high in developing grain, sheath, stem and seedlings but low in source leaves.
- Taxon
- rice
Oryza sativae0 - Gene
- OsSUT1e0
- Outcome
- highly expressed in developing grain, leaf sheath and stem after heading, and in germinating seedlingse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsSUT1 encodes a rice sucrose transport protein that is highly expressed in developing grain, leaf sheath and stem after heading, and in germinating seedlings, but only at very low levels in source leaves”
Claim · model-written summary
Many antisense rice lines had reduced filled grain production.
- Taxon
- riceOryza sativat0
- Treatment
- Antisense suppressiont0
- Outcome
- ability to produce filled graine0
- Direction
- reductione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Many of the plants showed a significant reduction in their ability to produce filled grain”
t0“Antisense suppression of the rice transporter gene”
t1“Antisense suppression of the rice transporter gene”
Claim · model-written summary
Final grain weight was reduced in antisense plants.
- Taxon
- riceOryza sativat0
- Treatment
- Antisense suppressiont0
- Outcome
- final grain weighte0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“final grain weight was reduced”
t0“Antisense suppression of the rice transporter gene”
t1“Antisense suppression of the rice transporter gene”
Claim · model-written summary
Severe phenotypes correlated with lower OsSUT1 transcript in filling grain.
- Taxon
- riceOryza sativat0
- Organ / stage
- filling graine0
- Gene
- OsSUT1e0
- Outcome
- Severe phenotypese0
- Direction
- reductione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Severe phenotypes correlated with a reduction in OsSUT1 transcript level in filling grain”
t0“Antisense suppression of the rice transporter gene”
t1“Antisense suppression of the rice transporter gene”
Claim · model-written summary
Source photosynthate supply was unaffected in antisense lines.
- Taxon
- riceOryza sativat0
- Treatment
- Antisense suppressiont0
- Outcome
- source supply of photosynthatee0
- Direction
- unaffectede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“source supply of photosynthate was unaffected in these transformants”
t0“Antisense suppression of the rice transporter gene”
t1“Antisense suppression of the rice transporter gene”
Claim · model-written summary
Seed germination rate was reduced in some T0 lines.
- Taxon
- riceOryza sativat0
- Material
- T0 populatione0
- Treatment
- Antisense suppressiont0
- Outcome
- rate of germinatione0
- Direction
- reductione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“seed from some of the T0 population showed a reduction in the rate of germination and growth”
t0“Antisense suppression of the rice transporter gene”
t1“Antisense suppression of the rice transporter gene”
Claim · model-written summary
Seedling growth was reduced in some T0 lines.
- Taxon
- riceOryza sativat0
- Material
- T0 populatione0
- Treatment
- Antisense suppressiont0
- Outcome
- growthe0
- Direction
- reductione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“seed from some of the T0 population showed a reduction in the rate of germination and growth”
t0“Antisense suppression of the rice transporter gene”
t1“Antisense suppression of the rice transporter gene”
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