Abstract
Despite its water-soluble chlorophyll-binding protein (WSCP) function, the putative trypsin inhibitor (TI) activity of the Brassica napus drought 22 kD (BnD22) protein and its physiological function in young leaves during leaf nitrogen (N) remobilization promoted by stressful conditions remains an enigma. Therefore, our objectives were to determine (1) if BnD22 is related to the 19-kD TI previously detected in B. napus young leaves, and (2) if the levels of BnD22 transcripts, BnD22 protein, and TI activity in young leaves are associated with plant responses to stress conditions (N starvation and methyl jasmonate [MeJA] treatments) that are able to modulate leaf senescence. Compared to control, N starvation delayed initiation of senescence and induced 19-kD TI activity in the young leaves. After 3 d with MeJA, the 19-kD TI activity was 7-fold higher than the control. Using two-dimensional electrophoresis gel, TI activity, and electrospray ionization liquid chromatography tandem mass spectrometry analysis, it was demonstrated that two 19-kD proteins with isoelectric points 5.0 and 5.1 harboring TI activity correspond to BnD22 perfectly. BnD22 gene expression, TI activities, and BnD22 protein presented similar patterns. Using polyclonal anti-WSCP antibodies of Brassica oleracea, six polypeptides separated by two-dimensional electrophoresis were detected in young leaves treated with MeJA. Electrospray ionization liquid chromatography tandem mass spectrometry analysis of six polypeptides confirms their homologies with WSCP. Results suggest that BnD22 possesses dual functions (WSCP and TI) that lead to the protection of younger tissues from adverse conditions by maintaining metabolism (protein integrity and photosynthesis). By sustaining sink growth of stressed plants, BnD22 may contribute to a better utilization of recycling N from sources, a physiological trait that improves N-use efficiency.
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Claims 10
Claim · model-written summary
Nitrogen starvation delayed senescence initiation in young Brassica napus leaves.
- Taxon
- Brassica napus
_Brassica_napus_-_Habit.jpg?width=60)
Brassica napuse1 - Organ / stage
- young leavese0
- Treatment
- N starvatione0
- Outcome
- initiation of senescencee0
- Direction
- delayede0
- Comparator
- controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Brassica_napus_-_Habit.jpg?width=500)
Verbatim
e0“Compared to control, N starvation delayed initiation of senescence and induced 19-kD TI activity in the young leaves.”
e1“Brassica napus drought 22 kD (BnD22) protein”
Claim · model-written summary
Nitrogen starvation induced 19-kD trypsin inhibitor activity in young Brassica napus leaves.
- Taxon
- Brassica napusBrassica napuse1
- Organ / stage
- young leavese0
- Treatment
- N starvatione0
- Outcome
- 19-kD TI activitye0
- Direction
- inducede0
- Comparator
- controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Compared to control, N starvation delayed initiation of senescence and induced 19-kD TI activity in the young leaves.”
e1“Brassica napus drought 22 kD (BnD22) protein”
Claim · model-written summary
After three days of MeJA, 19-kD TI activity was sevenfold higher than control.
- Taxon
- Brassica napusBrassica napuse1
- Treatment
- MeJAe0
- Outcome
- 19-kD TI activitye0
- Direction
- highere0
- Value
- 7-folde0
- Qualifier
- After 3 de0
- Comparator
- controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“After 3 d with MeJA, the 19-kD TI activity was 7-fold higher than the control.”
e1“Brassica napus drought 22 kD (BnD22) protein”
Claim · model-written summary
Two 19-kD proteins with trypsin inhibitor activity corresponded to BnD22.
- Taxon
- Brassica napusBrassica napuse1
- Gene
- BnD22e0
- Outcome
- harboring TI activity correspond to BnD22 perfectlye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“two 19-kD proteins with isoelectric points 5.0 and 5.1 harboring TI activity correspond to BnD22 perfectly”
e1“Brassica napus drought 22 kD (BnD22) protein”
Claim · model-written summary
BnD22 expression, trypsin inhibitor activity, and BnD22 protein showed similar patterns.
- Taxon
- Brassica napusBrassica napuse1
- Gene
- BnD22e0
- Outcome
- TI activitiese0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“BnD22 gene expression, TI activities, and BnD22 protein presented similar patterns.”
e1“Brassica napus drought 22 kD (BnD22) protein”
Claim · model-written summary
Six polypeptides were detected in MeJA-treated young leaves using anti-WSCP antibodies.
- Taxon
- Brassica napusBrassica napuse1
- Organ / stage
- young leavese0
- Treatment
- MeJAe0
- Outcome
- six polypeptidese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“six polypeptides separated by two-dimensional electrophoresis were detected in young leaves treated with MeJA.”
e1“Brassica napus drought 22 kD (BnD22) protein”
Claim · model-written summary
The six polypeptides had homology with WSCP.
- Taxon
- Brassica napusBrassica napuse1
- Gene
- WSCPe0
- Outcome
- homologies with WSCPe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“analysis of six polypeptides confirms their homologies with WSCP.”
e1“Brassica napus drought 22 kD (BnD22) protein”
Claim · model-written summary
The authors infer that BnD22 has both WSCP and trypsin inhibitor functions.
- Taxon
- Brassica napusBrassica napuse1
- Gene
- BnD22e0
- Outcome
- dual functions (WSCP and TI)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Results suggest that BnD22 possesses dual functions (WSCP and TI) that lead to the protection of younger tissues from adverse conditions by maintaining metabolism (protein integrity and photosynthesis).”
e1“Brassica napus drought 22 kD (BnD22) protein”
Claim · model-written summary
The authors propose that BnD22 protects younger tissues by maintaining protein integrity and photosynthesis.
- Taxon
- Brassica napusBrassica napuse1
- Gene
- BnD22e0
- Outcome
- protection of younger tissues from adverse conditionse0
- Qualifier
- maintaining metabolism (protein integrity and photosynthesis)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“BnD22 possesses dual functions (WSCP and TI) that lead to the protection of younger tissues from adverse conditions by maintaining metabolism (protein integrity and photosynthesis)”
e1“Brassica napus drought 22 kD (BnD22) protein”
Claim · model-written summary
The authors propose that BnD22 sustains sink growth and may improve nitrogen-use efficiency.
- Taxon
- Brassica napusBrassica napuse1
- Gene
- BnD22e0
- Outcome
- N-use efficiencye0
- Qualifier
- sustaining sink growth of stressed plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“By sustaining sink growth of stressed plants, BnD22 may contribute to a better utilization of recycling N from sources, a physiological trait that improves N-use efficiency.”
e1“Brassica napus drought 22 kD (BnD22) protein”
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See all 22 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.