Abstract
Ubiquitination is an important protein post-translational modification, which is involved in various cellular processes in higher plants, and U-box E3 ligases play important roles in diverse functions in eukaryotes. Here, we describe the functions of Arabidopsis thaliana PUB19 (AtPUB19), which we demonstrated in an in vitro assay to encode a U-box type E3 ubiquitin ligase. AtPUB19 was up-regulated by drought, salt, cold, and abscisic acid (ABA). Down-regulation of AtPUB19 led to hypersensitivity to ABA, enhanced ABA-induced stomatal closing, and enhanced drought tolerance, while AtPUB19 overexpression resulted in the reverse phenotypes. Molecular analysis showed that the expression levels of a number of ABA and stress marker genes were altered in both AtPUB19 overexpressing and atpub19-1 mutant plants. In summary, our data show that AtPUB19 negatively regulates ABA and drought responses in A. thaliana.
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Claims 11
Claim · model-written summary
AtPUB19 encoded a U-box type E3 ubiquitin ligase in vitro.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae0 - Gene
- AtPUB19e0
- Outcome
- U-box type E3 ubiquitin ligasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis thaliana PUB19 (AtPUB19), which we demonstrated in an in vitro assay to encode a U-box type E3 ubiquitin ligase”
Claim · model-written summary
Drought up-regulated AtPUB19.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- droughte0
- Gene
- AtPUB19e0
- Outcome
- AtPUB19e0
- Direction
- up-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“AtPUB19 was up-regulated by drought, salt, cold, and abscisic acid (ABA)”
t0“Arabidopsis thaliana”
Claim · model-written summary
Salt up-regulated AtPUB19.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- salte0
- Gene
- AtPUB19e0
- Outcome
- AtPUB19e0
- Direction
- up-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“AtPUB19 was up-regulated by drought, salt, cold, and abscisic acid (ABA)”
t0“Arabidopsis thaliana”
Claim · model-written summary
Cold up-regulated AtPUB19.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- colde0
- Gene
- AtPUB19e0
- Outcome
- AtPUB19e0
- Direction
- up-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“AtPUB19 was up-regulated by drought, salt, cold, and abscisic acid (ABA)”
t0“Arabidopsis thaliana”
Claim · model-written summary
ABA up-regulated AtPUB19.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- abscisic acid (ABA)e0
- Gene
- AtPUB19e0
- Outcome
- AtPUB19e0
- Direction
- up-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“AtPUB19 was up-regulated by drought, salt, cold, and abscisic acid (ABA)”
t0“Arabidopsis thaliana”
Claim · model-written summary
AtPUB19 down-regulation increased ABA sensitivity.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- Down-regulation of AtPUB19e0
- Gene
- AtPUB19e0
- Outcome
- hypersensitivity to ABAe0
- Direction
- hypersensitivitye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Down-regulation of AtPUB19 led to hypersensitivity to ABA, enhanced ABA-induced stomatal closing, and enhanced drought tolerance”
t0“Arabidopsis thaliana”
Claim · model-written summary
AtPUB19 down-regulation enhanced ABA-induced stomatal closing.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- Down-regulation of AtPUB19e0
- Gene
- AtPUB19e0
- Outcome
- ABA-induced stomatal closinge0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Down-regulation of AtPUB19 led to hypersensitivity to ABA, enhanced ABA-induced stomatal closing, and enhanced drought tolerance”
t0“Arabidopsis thaliana”
Claim · model-written summary
AtPUB19 down-regulation enhanced drought tolerance.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- Down-regulation of AtPUB19e0
- Gene
- AtPUB19e0
- Outcome
- drought tolerancee0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Down-regulation of AtPUB19 led to hypersensitivity to ABA, enhanced ABA-induced stomatal closing, and enhanced drought tolerance”
t0“Arabidopsis thaliana”
Claim · model-written summary
AtPUB19 overexpression produced the reverse ABA and drought phenotypes.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- AtPUB19 overexpressione0
- Gene
- AtPUB19e0
- Outcome
- reverse phenotypese0
- Direction
- reversee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“AtPUB19 overexpression resulted in the reverse phenotypes”
t0“Arabidopsis thaliana”
Claim · model-written summary
ABA and stress marker gene expression changed in AtPUB19 overexpressors and atpub19-1 mutants.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Gene
- ABA and stress marker genese0
- Outcome
- expression levelse0
- Direction
- alterede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“expression levels of a number of ABA and stress marker genes were altered in both AtPUB19 overexpressing and atpub19-1 mutant plants”
t0“Arabidopsis thaliana”
Claim · model-written summary
Authors conclude AtPUB19 negatively regulates ABA and drought responses.
- Taxon
- A. thalianaArabidopsis thalianae0
- Gene
- AtPUB19e0
- Outcome
- ABA and drought responsese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“AtPUB19 negatively regulates ABA and drought responses in A. thaliana”
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See all 76 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.