Abstract
Plasma membrane-enriched vesicles were isolated by density gradient centrifugation from roots of zinc-sufficient and zinc-deficient bean (Phaseolus vulgaris L. cv. Prélude) plants. The two populations of vesicles had similar activities of specific membrane marker enzymes and ATP hydrolysis and were competent for proton transport. However, vesicles from zinc-deficient roots showed lower rates of ATP-dependent intravesicular acidification and increases in passive permeability to protons as well as in the rate, of dissipation of a non-metabolic transmembrane pH gradient. The decrease of the rate of proton accumulation in isolated vesicles closely paralleled the increase in potassium leakage from intact roots and the appearance of visual zinc deficiency symptoms in the shoots. Re-supply of zinc to deficient plants for 24 h promoted shoot growth, reduced potassium leakage from roots and led to partial recovery of the proton accumulation capacity and to a decrease in passive permeability to protons in isolated vesicles. The results obtained with isolated vesicles confirm the previously observed ‘in vivo’ effects of zinc deficiency and are consistent with the idea that an alteration of plasma membrane lipids leads to an increase in permeability and an impairment in trans-plasma membrane proton gradient.
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Claims 10
Claim · model-written summary
Zinc-sufficient and deficient vesicles had similar marker enzyme and ATP hydrolysis activities.
- Taxon
- Phaseolus vulgaris L.
Phaseolus vulgarise1 - Material
- Préludee1
- Treatment
- zinc-deficiente1
- Outcome
- activities of specific membrane marker enzymes and ATP hydrolysise0
- Direction
- similare0
- Comparator
- zinc-sufficiente1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The two populations of vesicles had similar activities of specific membrane marker enzymes and ATP hydrolysis and were competent for proton transport.”
e1“roots of zinc-sufficient and zinc-deficient bean (Phaseolus vulgaris L. cv. Prélude) plants”
Claim · model-written summary
Both vesicle populations were competent for proton transport.
- Taxon
- Phaseolus vulgaris L.Phaseolus vulgarise1
- Outcome
- competent for proton transporte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The two populations of vesicles had similar activities of specific membrane marker enzymes and ATP hydrolysis and were competent for proton transport.”
e1“bean (Phaseolus vulgaris L. cv. Prélude)”
Claim · model-written summary
Zinc deficiency lowered ATP-dependent acidification rates.
- Taxon
- Phaseolus vulgaris L.Phaseolus vulgarise1
- Treatment
- zinc-deficiente0
- Outcome
- rates of ATP-dependent intravesicular acidificatione0
- Direction
- lowere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“vesicles from zinc-deficient roots showed lower rates of ATP-dependent intravesicular acidification”
e1“bean (Phaseolus vulgaris L. cv. Prélude)”
Claim · model-written summary
Zinc deficiency increased passive proton permeability and pH gradient dissipation.
- Taxon
- Phaseolus vulgaris L.Phaseolus vulgarise1
- Treatment
- zinc-deficiente0
- Outcome
- passive permeability to protons as well as in the rate, of dissipation of a non-metabolic transmembrane pH gradiente0
- Direction
- increasese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“vesicles from zinc-deficient roots showed lower rates of ATP-dependent intravesicular acidification and increases in passive permeability to protons as well as in the rate, of dissipation of a non-metabolic transmembrane pH gradient”
e1“bean (Phaseolus vulgaris L. cv. Prélude)”
Claim · model-written summary
Decreased proton accumulation paralleled potassium leakage increase and shoot deficiency symptoms.
- Taxon
- Phaseolus vulgaris L.Phaseolus vulgarise1
- Organ / stage
- intact rootse0
- Outcome
- rate of proton accumulatione0
- Direction
- decreasee0
- Qualifier
- increase in potassium leakage from intact roots and the appearance of visual zinc deficiency symptoms in the shootse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The decrease of the rate of proton accumulation in isolated vesicles closely paralleled the increase in potassium leakage from intact roots and the appearance of visual zinc deficiency symptoms in the shoots.”
e1“bean (Phaseolus vulgaris L. cv. Prélude)”
Claim · model-written summary
Zinc resupply for 24 h promoted shoot growth.
- Taxon
- Phaseolus vulgaris L.Phaseolus vulgarise1
- Treatment
- Re-supply of zince0
- Outcome
- shoot growthe0
- Direction
- promotede0
- Qualifier
- 24 he0
- Comparator
- deficient plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Re-supply of zinc to deficient plants for 24 h promoted shoot growth, reduced potassium leakage from roots”
e1“bean (Phaseolus vulgaris L. cv. Prélude)”
Claim · model-written summary
Zinc resupply reduced root potassium leakage.
- Taxon
- Phaseolus vulgaris L.Phaseolus vulgarise1
- Treatment
- Re-supply of zince0
- Outcome
- potassium leakage from rootse0
- Direction
- reducede0
- Qualifier
- 24 he0
- Comparator
- deficient plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Re-supply of zinc to deficient plants for 24 h promoted shoot growth, reduced potassium leakage from roots”
e1“bean (Phaseolus vulgaris L. cv. Prélude)”
Claim · model-written summary
Zinc resupply partially restored proton accumulation capacity.
- Taxon
- Phaseolus vulgaris L.Phaseolus vulgarise1
- Treatment
- Re-supply of zince0
- Outcome
- proton accumulation capacitye0
- Direction
- partial recoverye0
- Qualifier
- 24 he0
- Comparator
- deficient plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Re-supply of zinc to deficient plants for 24 h promoted shoot growth, reduced potassium leakage from roots and led to partial recovery of the proton accumulation capacity and to a decrease in passive permeability to protons in isolated vesicles.”
e1“bean (Phaseolus vulgaris L. cv. Prélude)”
Claim · model-written summary
Zinc resupply decreased passive proton permeability.
- Taxon
- Phaseolus vulgaris L.Phaseolus vulgarise1
- Treatment
- Re-supply of zince0
- Outcome
- passive permeability to protonse0
- Direction
- decreasee0
- Qualifier
- 24 he0
- Comparator
- deficient plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Re-supply of zinc to deficient plants for 24 h promoted shoot growth, reduced potassium leakage from roots and led to partial recovery of the proton accumulation capacity and to a decrease in passive permeability to protons in isolated vesicles.”
e1“bean (Phaseolus vulgaris L. cv. Prélude)”
Claim · model-written summary
Results support lipid alteration as an explanation for increased permeability and impaired proton gradient.
- Taxon
- Phaseolus vulgaris L.Phaseolus vulgarise1
- Outcome
- increase in permeability and an impairment in trans-plasma membrane proton gradiente0
- Qualifier
- alteration of plasma membrane lipidse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“consistent with the idea that an alteration of plasma membrane lipids leads to an increase in permeability and an impairment in trans-plasma membrane proton gradient”
e1“bean (Phaseolus vulgaris L. cv. Prélude)”
The same questions in other papers
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- negative
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8 papers in the same direction as this paper · 6 in the opposite direction
- positive
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- negative
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See all 15 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.