Abstract
Iron (Fe) and zinc (Zn) stress significantly affects fundamental metabolic and physiological processes in plants that results in reduction of plant growth and development. In the present study, common bean variety; Shalimar French Bean-1 (SFB-1) was used as an experimental material. Four different MGRL media i.e. normal MGRL medium (Control), media without Fe (0-Fe), media without Zn (0-Zn) and media with excess Zn (300-Zn) were used for growing seeds of SFB-1 under in vitro condition for three weeks under optimum conditions. Three week old shoot and root tissues were harvested from the plants grown in these four different in vitro conditions and were, subjected to Fe and Zn estimation. Further, extraction of total RNA for differential gene expression of ten candidate genes selected based on our in silico investigation and their classification, phylogeny and expression pattern was unraveled. Expression analysis of three candidate genes (OPT3, NRAMP2 and NRAMP3) in roots revealed possible cross talk among Fe/Zn stress that was further confirmed by observing less accumulation of Fe in roots under both these conditions. However, we observed, higher accumulation of Fe in shoots under 0-Fe condition compared to control that suggests precise sensing for priority based compartmentalization and partitioning leading to higher accumulation of Fe in shoots. Furthermore, the expression analysis of IRT1, FRO1 and Ferritin 1 genes under Fe/Zn stress suggested their role in uptake/transport and signaling of Fe and Zn, whereas the expression of ZIP2, NRAMP1, HA2 and GLP1 genes were highly responsive to Zn in Phaseolus vulgaris. The identified genes highly responsive to Fe and Zn stress condition can be potential candidates for overcoming mineral stress in dicot crop plants.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 5
Claim · model-written summary
OPT3, NRAMP2 and NRAMP3 root expression suggested Fe/Zn stress cross talk.
- Taxon
- common bean
Phaseolus vulgarist0 - Organ / stage
- rootse0
- Gene
- OPT3, NRAMP2 and NRAMP3e0
- Outcome
- possible cross talk among Fe/Zn stresse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“common bean (Phaseolus vulgaris L.)”
e0“Expression analysis of three candidate genes (OPT3, NRAMP2 and NRAMP3) in roots revealed possible cross talk among Fe/Zn stress that was further confirmed by observing less accumulation of Fe in roots under both these conditions.”
Claim · model-written summary
Roots accumulated less Fe under both Fe and Zn stress conditions.
- Taxon
- common beanPhaseolus vulgarist0
- Organ / stage
- rootse0
- Treatment
- Fe/Zn stresse0
- Outcome
- accumulation of Fe in rootse0
- Direction
- lesse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“common bean (Phaseolus vulgaris L.)”
e0“Expression analysis of three candidate genes (OPT3, NRAMP2 and NRAMP3) in roots revealed possible cross talk among Fe/Zn stress that was further confirmed by observing less accumulation of Fe in roots under both these conditions.”
Claim · model-written summary
Shoot Fe accumulation was higher under 0-Fe than control conditions.
- Taxon
- common beanPhaseolus vulgarist0
- Organ / stage
- shootse0
- Treatment
- 0-Fee0
- Outcome
- accumulation of Fe in shootse0
- Direction
- highere0
- Comparator
- controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“common bean (Phaseolus vulgaris L.)”
e0“higher accumulation of Fe in shoots under 0-Fe condition compared to control”
Claim · model-written summary
IRT1, FRO1 and Ferritin 1 expression under Fe/Zn stress suggested roles in Fe and Zn uptake, transport and signaling.
- Taxon
- common beanPhaseolus vulgarist0
- Treatment
- Fe/Zn stresse0
- Gene
- IRT1, FRO1 and Ferritin 1e0
- Outcome
- uptake/transport and signaling of Fe and Zne0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“common bean (Phaseolus vulgaris L.)”
e0“the expression analysis of IRT1, FRO1 and Ferritin 1 genes under Fe/Zn stress suggested their role in uptake/transport and signaling of Fe and Zn”
Claim · model-written summary
ZIP2, NRAMP1, HA2 and GLP1 expression was highly responsive to Zn.
- Taxon
- Phaseolus vulgarisPhaseolus vulgarise0
- Treatment
- Zne0
- Gene
- ZIP2, NRAMP1, HA2 and GLP1e0
- Outcome
- expressione0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“common bean (Phaseolus vulgaris L.)”
e0“the expression of ZIP2, NRAMP1, HA2 and GLP1 genes were highly responsive to Zn in Phaseolus vulgaris”
The same questions in other papers
iron atom · molecular: metabolites · Phaseolus vulgaris (common bean)Positive and negative results
1 paper in the same direction as this paper · 6 in the opposite direction
- positive
- positive
- positive
4 papers more in this group
See all 8 papers →zinc atom · molecular: metabolites · Phaseolus vulgaris (common bean)Positive and negative results
5 papers in the same direction as this paper · 9 in the opposite direction
- positive
- positive
- positive
11 papers more in this group
See all 15 papers →heavy metal · molecular: metabolites · Phaseolus vulgaris (common bean)Positive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- positive
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.