Abstract
The stay green mutant genotype d1d1d2d2 inhibits the breakdown of chloroplast components in senescing leaves of soybean (Glycine max L. Merr.). Together with G (a gene that preserves chlorophyll in the seed coat) they may extend photosynthetic activity in some conditions. While wild-type soybeans maintain high leaf water potentials right up to abscission, leaves of (GG)d1d1d2d2 dehydrate late in senescence, which suggests that water relations may be altered in the mutant. Three-week-old plants were subjected to a moderate water deficit (soil water potential=-0.7 MPa) for 7-10 d. Leaf water potential and relative water content decreased significantly more in response to water deficit in unifoliate leaves of GGd1d1d2d2 than in a near-isogenic wild-type line. Down-regulation of stomatal conductance in response to drought was similar in mutant and wild-type leaves. Likewise, exogenously applied ABA reduced stomatal conductance to a similar extent in the mutant and the wild type, and applied ABA failed to restore water deficit tolerance in GGd1d1d2d2. Experiments with explants lacking roots indicate that the accelerated dehydration of GGd1d1d2d2 is probably not due to alterations in the roots. In a comparison of near-isogenic lines carrying different combinations of d1, d2 and G, only d1d1d2d2 and GGd1d1d2d2 (i.e. the genotypes that cause the stay green phenotype) were more susceptible to water deficit than the wild type. These data suggest that pathways involved in chloroplast disassembly and in the regulation of stress responses may be intertwined and controlled by the same factors.
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Claims 7
Claim · model-written summary
GGd1d1d2d2 leaves dehydrated late in senescence while wild-type leaves retained high water potential.
- Taxon
- soybeans
Glycine maxe0 - Material
- (GG)d1d1d2d2e0
- Outcome
- dehydrate late in senescencee0
- Comparator
- wild-typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“While wild-type soybeans maintain high leaf water potentials right up to abscission, leaves of (GG)d1d1d2d2 dehydrate late in senescence”
Claim · model-written summary
Under moderate water deficit, mutant leaf water potential and relative water content fell significantly more than in wild type.
- Taxon
- soybeansGlycine maxt0
- Material
- GGd1d1d2d2e0
- Treatment
- water deficite0
- Outcome
- Leaf water potential and relative water contente0
- Direction
- decreasede0
- Comparator
- near-isogenic wild-type linee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Leaf water potential and relative water content decreased significantly more in response to water deficit in unifoliate leaves of GGd1d1d2d2 than in a near-isogenic wild-type line”
t0“soybeans”
Claim · model-written summary
Stomatal conductance down-regulation under drought was similar in mutant and wild type.
- Taxon
- soybeansGlycine maxt0
- Material
- mutante0
- Treatment
- droughte0
- Outcome
- stomatal conductancee0
- Direction
- similare0
- Comparator
- wild-type leavese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Down-regulation of stomatal conductance in response to drought was similar in mutant and wild-type leaves”
t0“soybeans”
Claim · model-written summary
Applied ABA reduced stomatal conductance similarly in mutant and wild type.
- Taxon
- soybeansGlycine maxt0
- Material
- mutante0
- Treatment
- exogenously applied ABAe0
- Outcome
- stomatal conductancee0
- Direction
- reducede0
- Comparator
- wild typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“exogenously applied ABA reduced stomatal conductance to a similar extent in the mutant and the wild type”
t0“soybeans”
Claim · model-written summary
Applied ABA failed to restore water deficit tolerance in GGd1d1d2d2.
- Taxon
- soybeansGlycine maxt0
- Material
- GGd1d1d2d2e0
- Treatment
- applied ABAe0
- Outcome
- water deficit tolerancee0
- Direction
- failed to restoree0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“applied ABA failed to restore water deficit tolerance in GGd1d1d2d2”
t0“soybeans”
Claim · model-written summary
Explant experiments suggested that mutant accelerated dehydration was probably not due to root alterations.
- Taxon
- soybeansGlycine maxt0
- Material
- GGd1d1d2d2e0
- Organ / stage
- rootse0
- Outcome
- accelerated dehydratione0
- Qualifier
- probably not due to alterations in the rootse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Experiments with explants lacking roots indicate that the accelerated dehydration of GGd1d1d2d2 is probably not due to alterations in the roots”
t0“soybeans”
Claim · model-written summary
Only the two stay-green d1d1d2d2 genotypes were more susceptible to water deficit than wild type.
- Taxon
- soybeansGlycine maxt0
- Material
- d1d1d2d2 and GGd1d1d2d2e0
- Outcome
- susceptible to water deficite0
- Direction
- moree0
- Comparator
- wild typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“only d1d1d2d2 and GGd1d1d2d2 (i.e. the genotypes that cause the stay green phenotype) were more susceptible to water deficit than the wild type”
t0“soybeans”
The same questions in other papers
(+)-abscisic acid · disease · Glycine max (soybean)Positive and negative results
4 in the opposite direction
- positive
- positive
- positive
1 paper more in this group
See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.