Abstract
Tubers of transgenic potato (Solanum tuberosum) plants with decreased activity of the plastidic ATP/ADP transporter AATP1 display reduced levels of starch, modified tuber morphology, and altered concentrations of primary metabolites. Here, we demonstrate that the spontaneous production of hydrogen peroxide, the endogenous content of salicylic acid, and the levels of mRNAs of various defense-related genes are similar in tuber discs of wild-type and AATP1(St) antisense plants. However, upon challenging the tissue with fungal elicitors or culture supernatants of the soft rot-causing pathogen Erwinia carotovora subsp. atroseptica, the AATP1(St) antisense tubers exhibit highly potentiated activation of defense responses when compared with wild-type tissue. The augmented defense responses comprise enhanced accumulation of transcripts of five defense-related genes (beta-1,3-GLUCANASE B2 and A1, CHITINASE B3 and A2, and Phe AMMONIA-LYASE) and enhanced elicitation (up to 21-fold) of the early hydrogen peroxide burst. The potentiated activation of cellular defense responses in AATP1(St) antisense tubers is not accompanied by a precedent increase in endogenous salicylic acid levels, but is associated with a strongly enhanced resistance of the tissue to E. carotovora. From these results, we conclude that inhibition of primary metabolic reactions induces a primed state that sensitizes the potato tubers for improved elicitation of various cellular defense responses, which likely contribute to enhanced E. carotovora resistance.
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Claims 11
Claim · model-written summary
Decreased AATP1 activity in transgenic potato tubers reduced starch levels.
- Taxon
- Solanum tuberosum
Solanum tuberosume1 - Material
- transgenic potatoe1
- Organ / stage
- Tuberse0
- Treatment
- decreased activity of the plastidic ATP/ADP transporter AATP1e0
- Gene
- AATP1e0
- Outcome
- levels of starche0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Tubers of transgenic potato (Solanum tuberosum) plants with decreased activity of the plastidic ATP/ADP transporter AATP1 display reduced levels of starch, modified tuber morphology, and altered concentrations of primary metabolites”
e1“transgenic potato (Solanum tuberosum)”
Claim · model-written summary
Decreased AATP1 activity modified tuber morphology.
- Taxon
- Solanum tuberosumSolanum tuberosume1
- Material
- transgenic potatoe1
- Organ / stage
- Tuberse0
- Treatment
- decreased activity of the plastidic ATP/ADP transporter AATP1e0
- Gene
- AATP1e0
- Outcome
- tuber morphologye0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Tubers of transgenic potato (Solanum tuberosum) plants with decreased activity of the plastidic ATP/ADP transporter AATP1 display reduced levels of starch, modified tuber morphology, and altered concentrations of primary metabolites”
e1“transgenic potato (Solanum tuberosum)”
Claim · model-written summary
Decreased AATP1 activity altered concentrations of primary metabolites in potato tubers.
- Taxon
- Solanum tuberosumSolanum tuberosume1
- Material
- transgenic potatoe1
- Organ / stage
- Tuberse0
- Treatment
- decreased activity of the plastidic ATP/ADP transporter AATP1e0
- Gene
- AATP1e0
- Outcome
- concentrations of primary metabolitese0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Tubers of transgenic potato (Solanum tuberosum) plants with decreased activity of the plastidic ATP/ADP transporter AATP1 display reduced levels of starch, modified tuber morphology, and altered concentrations of primary metabolites”
e1“transgenic potato (Solanum tuberosum)”
Claim · model-written summary
Hydrogen peroxide production, salicylic acid content and defense-gene mRNA levels in tuber discs were similar between wild-type and AATP1(St) antisense plants.
- Taxon
- Solanum tuberosumSolanum tuberosume1
- Material
- AATP1(St) antisensee0
- Organ / stage
- tuber discse0
- Treatment
- AATP1(St) antisensee0
- Outcome
- spontaneous production of hydrogen peroxide, the endogenous content of salicylic acid, and the levels of mRNAs of various defense-related genese0
- Direction
- similare0
- Comparator
- wild-typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Here, we demonstrate that the spontaneous production of hydrogen peroxide, the endogenous content of salicylic acid, and the levels of mRNAs of various defense-related genes are similar in tuber discs of wild-type and AATP1(St) antisense plants”
e1“transgenic potato (Solanum tuberosum)”
Claim · model-written summary
AATP1(St) antisense tubers showed highly potentiated activation of defense responses compared with wild-type tissue after challenge with fungal elicitors or Erwinia culture supernatants.
- Taxon
- Solanum tuberosumSolanum tuberosume1
- Material
- AATP1(St) antisensee0
- Treatment
- challenging the tissue with fungal elicitors or culture supernatantse0
- Interactor
- Erwinia carotovora subsp. atroseptica
Pectobacterium atrosepticume0pathogen - Outcome
- activation of defense responsese0
- Direction
- highly potentiatede0
- Comparator
- wild-type tissuee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“However, upon challenging the tissue with fungal elicitors or culture supernatants of the soft rot-causing pathogen Erwinia carotovora subsp. atroseptica, the AATP1(St) antisense tubers exhibit highly potentiated activation of defense responses when compared with wild-type tissue”
e1“transgenic potato (Solanum tuberosum)”
Claim · model-written summary
AATP1(St) antisense tubers accumulated more transcripts of beta-1,3-GLUCANASE B2 and A1.
- Taxon
- Solanum tuberosumSolanum tuberosume2
- Material
- AATP1(St) antisensee1
- Treatment
- AATP1(St) antisensee1
- Gene
- beta-1,3-GLUCANASE B2 and A1e0
- Outcome
- accumulation of transcriptse0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The augmented defense responses comprise enhanced accumulation of transcripts of five defense-related genes (beta-1,3-GLUCANASE B2 and A1, CHITINASE B3 and A2, and Phe AMMONIA-LYASE) and enhanced elicitation (up to 21-fold) of the early hydrogen peroxide burst”
e1“the AATP1(St) antisense tubers exhibit highly potentiated activation”
e2“transgenic potato (Solanum tuberosum)”
Claim · model-written summary
AATP1(St) antisense tubers accumulated more transcripts of CHITINASE B3 and A2, and Phe AMMONIA-LYASE.
- Taxon
- Solanum tuberosumSolanum tuberosume2
- Material
- AATP1(St) antisensee1
- Treatment
- AATP1(St) antisensee1
- Gene
- CHITINASE B3 and A2, and Phe AMMONIA-LYASEe0
- Outcome
- accumulation of transcriptse0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The augmented defense responses comprise enhanced accumulation of transcripts of five defense-related genes (beta-1,3-GLUCANASE B2 and A1, CHITINASE B3 and A2, and Phe AMMONIA-LYASE) and enhanced elicitation (up to 21-fold) of the early hydrogen peroxide burst”
e1“the AATP1(St) antisense tubers exhibit highly potentiated activation”
e2“transgenic potato (Solanum tuberosum)”
Claim · model-written summary
Elicitation of the early hydrogen peroxide burst was enhanced up to 21-fold in AATP1(St) antisense tubers.
- Taxon
- Solanum tuberosumSolanum tuberosume2
- Material
- AATP1(St) antisensee1
- Treatment
- AATP1(St) antisensee1
- Outcome
- early hydrogen peroxide burste0
- Direction
- enhancede0
- Value
- up to 21-folde0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The augmented defense responses comprise enhanced accumulation of transcripts of five defense-related genes (beta-1,3-GLUCANASE B2 and A1, CHITINASE B3 and A2, and Phe AMMONIA-LYASE) and enhanced elicitation (up to 21-fold) of the early hydrogen peroxide burst”
e1“the AATP1(St) antisense tubers exhibit highly potentiated activation”
e2“transgenic potato (Solanum tuberosum)”
Claim · model-written summary
AATP1(St) antisense tubers showed no precedent increase in endogenous salicylic acid levels.
- Taxon
- Solanum tuberosumSolanum tuberosume1
- Material
- AATP1(St) antisensee0
- Treatment
- AATP1(St) antisensee0
- Outcome
- endogenous salicylic acid levelse0
- Direction
- no_changeinferrede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The potentiated activation of cellular defense responses in AATP1(St) antisense tubers is not accompanied by a precedent increase in endogenous salicylic acid levels, but is associated with a strongly enhanced resistance of the tissue to E. carotovora”
e1“transgenic potato (Solanum tuberosum)”
Claim · model-written summary
AATP1(St) antisense tubers had a strongly enhanced resistance to E. carotovora.
- Taxon
- Solanum tuberosumSolanum tuberosume1
- Material
- AATP1(St) antisensee0
- Treatment
- AATP1(St) antisensee0
- Interactor
- E. carotovora
Pectobacterium carotovorume0pathogen - Outcome
- resistance of the tissue to E. carotovorae0
- Direction
- strongly enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The potentiated activation of cellular defense responses in AATP1(St) antisense tubers is not accompanied by a precedent increase in endogenous salicylic acid levels, but is associated with a strongly enhanced resistance of the tissue to E. carotovora”
e1“transgenic potato (Solanum tuberosum)”
Claim · model-written summary
The authors conclude that inhibition of primary metabolic reactions primes potato tubers for improved defense elicitation, which likely contributes to enhanced E. carotovora resistance.
- Taxon
- potatoSolanum tuberosume0
- Treatment
- inhibition of primary metabolic reactionse0
- Outcome
- improved elicitation of various cellular defense responsese0
- Qualifier
- primed statee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“From these results, we conclude that inhibition of primary metabolic reactions induces a primed state that sensitizes the potato tubers for improved elicitation of various cellular defense responses, which likely contribute to enhanced E. carotovora resistance”
e1“transgenic potato (Solanum tuberosum)”
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See all 17 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.