Abstract
UNLABELLED: Zinc (Zn) is an essential micronutrient required for plant growth and development. In Arabidopsis thaliana, several families of Zn transporters engaged in Zn import, export and intracellular compartmentalization play important roles in Zn homeostasis. We describe a novel Zn transporter, A. thaliana metal tolerance protein 12 (AtMTP12), which belongs to the cation diffusion facilitator family. AtMTP12 is predicted to consist of 798 amino acids and have 14 transmembrane segments. The expression of AtMTP12 in suspension-cultured cells was not affected by Zn deficiency or excess. Heterologous expression in a mutant of budding yeast (Saccharomyces cerevisiae) that lacks Msc2p, an orthologue of AtMTP12, revealed that AtMTP12 complements the growth phenotype of the msc2 mutant when AtMTP5t1, one of the splicing variants of AtMTP5, is coexpressed. Transient expression of AtMTP12-fused green fluorescent protein in A. thaliana mesophyll protoplasts demonstrated that AtMTP12 is localized to the Golgi apparatus. Moreover, AtMTP12 and AtMTP5t1 interact in the Golgi, as determined by a bimolecular fluorescence complementation assay. These results suggest that AtMTP12 forms a functional complex with AtMTP5t1 to transport Zn into the Golgi. DATABASE: Nucleotide sequence data for full-length of AtMTP12 is available in the DDBJ/EMBL/GenBank database under accession number AB986563.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 7
Claim · model-written summary
AtMTP12 was identified as a cation diffusion facilitator zinc transporter.
- Taxon
- A. thaliana
Arabidopsis thalianae0 - Gene
- A. thaliana metal tolerance protein 12 (AtMTP12)e0
- Outcome
- belongs to the cation diffusion facilitator familye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a novel Zn transporter, A. thaliana metal tolerance protein 12 (AtMTP12), which belongs to the cation diffusion facilitator family”
Claim · model-written summary
AtMTP12 was predicted to have 798 amino acids and 14 transmembrane segments.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Gene
- AtMTP12e0
- Outcome
- 798 amino acids and have 14 transmembrane segmentse0
- Value
- 798 amino acidse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“AtMTP12 is predicted to consist of 798 amino acids and have 14 transmembrane segments”
t0“Arabidopsis thaliana”
Claim · model-written summary
Zinc deficiency or excess did not affect AtMTP12 expression in cultured cells.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Organ / stage
- suspension-cultured cellse0
- Treatment
- Zn deficiency or excesse0
- Gene
- AtMTP12e0
- Outcome
- expression of AtMTP12e0
- Direction
- not affectede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The expression of AtMTP12 in suspension-cultured cells was not affected by Zn deficiency or excess”
t0“Arabidopsis thaliana”
Claim · model-written summary
AtMTP12 complemented yeast msc2 growth when AtMTP5t1 was coexpressed.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- AtMTP5t1e0
- Gene
- AtMTP12e0
- Outcome
- growth phenotype of the msc2 mutante0
- Direction
- complementse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“AtMTP12 complements the growth phenotype of the msc2 mutant when AtMTP5t1, one of the splicing variants of AtMTP5, is coexpressed”
t0“Arabidopsis thaliana”
Claim · model-written summary
AtMTP12 localized to the Golgi apparatus.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Gene
- AtMTP12e0
- Outcome
- localized to the Golgi apparatuse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“AtMTP12 is localized to the Golgi apparatus”
t0“Arabidopsis thaliana”
Claim · model-written summary
AtMTP12 and AtMTP5t1 interacted in the Golgi.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Gene
- AtMTP12 and AtMTP5t1e0
- Outcome
- interact in the Golgie0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“AtMTP12 and AtMTP5t1 interact in the Golgi”
t0“Arabidopsis thaliana”
Claim · model-written summary
The authors infer an AtMTP12–AtMTP5t1 complex transports zinc into the Golgi.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Gene
- AtMTP12e0
- Outcome
- transport Zn into the Golgie0
- Qualifier
- forms a functional complex with AtMTP5t1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“AtMTP12 forms a functional complex with AtMTP5t1 to transport Zn into the Golgi”
t0“Arabidopsis thaliana”
The same questions in other papers
zinc atom · molecular: expression · Arabidopsis thaliana (thale cress)Positive and negative results
3 papers in the same direction as this paper · 2 in the opposite direction
This paper is about lacking the nutrient, so it counts the other way round here.
- positive
- positive
- no effect
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.