Abstract
A synthetic brassinosteroid, 22,23(S,S)-homobrassinolide (hBR), was examined for its interaction with IAA and GA3 in the elongation of hypocotyl sections of light-grown cucumber (Cucumis salivus L. cv. Aonagajibai) seedlings. hBR alone was less active than IAA. Its optimal concentration was around 10 μm and the lowest effective concentration between 10 and 100 μm, which is more than 100 times higher than that of brassinolide. hBR was more active in sections from younger seedlings. Its growth-promoting effect was negated or greatly reduced by inhibitors of auxin-induced elongation such as p-chlorophenoxyisobutyric acid and kinetin. hBR acted synergistically with IAA and 2,4-D but not with GA3 showing only an additive effect. Sequential treatment of sections with hBR and then with IAA also resulted in synergistic enhancement of auxininduced elongation, but when the order of treatment was reversed, hBR was inactive. The synergistic effect was obtained with 1 h pretreatment with hBR and could be reduced by subsequent washing with water. There was no sequential interaction between hBR and GA3. The synergistic pretreatment effects of hBR and GA3 were simply additive to each other. A membrane-bound ATPase inhibitor, dicyclohexylcarbodiimide, inhibited the hBR-induced elongation, but did not affect GA3-induced elongation. The findings led to the conclusion that brassinosteroids enhance auxin action and possess growth-promoting activity which is independent of that of gibberellin.
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Claims 18
Claim · model-written summary
hBR was less active than IAA in cucumber hypocotyl elongation.
- Taxon
- Cucumber
Cucumis sativust0 - Organ / stage
- Hypocotyl Sectionst0
- Treatment
- hBRe0
- Outcome
- activee0
- Direction
- lesse0
- Comparator
- IAAe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“hBR alone was less active than IAA.”
t0“Cucumber Hypocotyl Sections”
Claim · model-written summary
hBR had an optimal concentration around 10 μm.
- Taxon
- CucumberCucumis sativust0
- Outcome
- optimal concentratione0
- Value
- 10 μme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Its optimal concentration was around 10 μm”
t0“Cucumber Hypocotyl Sections”
Claim · model-written summary
The lowest effective hBR concentration was between 10 and 100 μm, more than 100 times that of brassinolide.
- Taxon
- CucumberCucumis sativust0
- Outcome
- lowest effective concentratione0
- Direction
- highere0
- Value
- 10 and 100 μme0
- Qualifier
- more than 100 timese0
- Comparator
- brassinolidee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the lowest effective concentration between 10 and 100 μm, which is more than 100 times higher than that of brassinolide.”
t0“Cucumber Hypocotyl Sections”
Claim · model-written summary
hBR was more active in hypocotyl sections from younger seedlings.
- Taxon
- CucumberCucumis sativust0
- Organ / stage
- younger seedlingse0
- Treatment
- hBRe0
- Outcome
- activee0
- Direction
- moree0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“hBR was more active in sections from younger seedlings.”
t0“Cucumber Hypocotyl Sections”
Claim · model-written summary
p-chlorophenoxyisobutyric acid negated or greatly reduced hBR growth promotion.
- Taxon
- CucumberCucumis sativust0
- Treatment
- p-chlorophenoxyisobutyric acide0
- Outcome
- growth-promoting effecte0
- Direction
- negated or greatly reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Its growth-promoting effect was negated or greatly reduced by inhibitors of auxin-induced elongation such as p-chlorophenoxyisobutyric acid and kinetin.”
t0“Cucumber Hypocotyl Sections”
Claim · model-written summary
Kinetin negated or greatly reduced hBR growth promotion.
- Taxon
- CucumberCucumis sativust0
- Treatment
- kinetine0
- Outcome
- growth-promoting effecte0
- Direction
- negated or greatly reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Its growth-promoting effect was negated or greatly reduced by inhibitors of auxin-induced elongation such as p-chlorophenoxyisobutyric acid and kinetin.”
t0“Cucumber Hypocotyl Sections”
Claim · model-written summary
hBR acted synergistically with IAA on elongation.
- Taxon
- CucumberCucumis sativust0
- Treatment
- hBRe0
- Outcome
- synergisticallye0
- Comparator
- IAAe0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“hBR acted synergistically with IAA and 2,4-D but not with GA3 showing only an additive effect.”
t0“Cucumber Hypocotyl Sections”
Claim · model-written summary
hBR acted synergistically with 2,4-D on elongation.
- Taxon
- CucumberCucumis sativust0
- Treatment
- hBRe0
- Outcome
- synergisticallye0
- Comparator
- 2,4-De0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“hBR acted synergistically with IAA and 2,4-D but not with GA3 showing only an additive effect.”
t0“Cucumber Hypocotyl Sections”
Claim · model-written summary
hBR and GA3 showed only an additive effect.
- Taxon
- CucumberCucumis sativust0
- Treatment
- hBRe0
- Outcome
- additive effecte0
- Comparator
- GA3e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“hBR acted synergistically with IAA and 2,4-D but not with GA3 showing only an additive effect.”
t0“Cucumber Hypocotyl Sections”
Claim · model-written summary
hBR pretreatment followed by IAA synergistically enhanced auxin-induced elongation.
- Taxon
- CucumberCucumis sativust0
- Treatment
- hBR and then with IAAe0
- Outcome
- auxininduced elongatione0
- Direction
- enhancemente0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Sequential treatment of sections with hBR and then with IAA also resulted in synergistic enhancement of auxininduced elongation”
t0“Cucumber Hypocotyl Sections”
Claim · model-written summary
Reversing the order to IAA then hBR left hBR inactive.
- Taxon
- CucumberCucumis sativust0
- Treatment
- order of treatment was reversede0
- Outcome
- hBR was inactivee0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“but when the order of treatment was reversed, hBR was inactive.”
t0“Cucumber Hypocotyl Sections”
Claim · model-written summary
One hour of hBR pretreatment yielded a synergistic effect.
- Taxon
- CucumberCucumis sativust0
- Treatment
- 1 h pretreatment with hBRe0
- Outcome
- synergistic effecte0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The synergistic effect was obtained with 1 h pretreatment with hBR”
t0“Cucumber Hypocotyl Sections”
Claim · model-written summary
Subsequent washing with water reduced the synergistic effect.
- Taxon
- CucumberCucumis sativust0
- Treatment
- subsequent washing with watere0
- Outcome
- reducede0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“could be reduced by subsequent washing with water.”
t0“Cucumber Hypocotyl Sections”
Claim · model-written summary
No sequential interaction occurred between hBR and GA3.
- Taxon
- CucumberCucumis sativust0
- Outcome
- no sequential interaction between hBR and GA3e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“There was no sequential interaction between hBR and GA3.”
t0“Cucumber Hypocotyl Sections”
Claim · model-written summary
The synergistic pretreatment effects of hBR and GA3 were additive to each other.
- Taxon
- CucumberCucumis sativust0
- Outcome
- simply additive to each othere0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The synergistic pretreatment effects of hBR and GA3 were simply additive to each other.”
t0“Cucumber Hypocotyl Sections”
Claim · model-written summary
Dicyclohexylcarbodiimide inhibited hBR-induced elongation.
- Taxon
- CucumberCucumis sativust0
- Treatment
- dicyclohexylcarbodiimidee0
- Gene
- ATPasee0
- Outcome
- hBR-induced elongatione0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A membrane-bound ATPase inhibitor, dicyclohexylcarbodiimide, inhibited the hBR-induced elongation”
t0“Cucumber Hypocotyl Sections”
Claim · model-written summary
Dicyclohexylcarbodiimide did not affect GA3-induced elongation.
- Taxon
- CucumberCucumis sativust0
- Treatment
- dicyclohexylcarbodiimidee0
- Outcome
- GA3-induced elongatione0
- Direction
- did not affecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“dicyclohexylcarbodiimide, inhibited the hBR-induced elongation, but did not affect GA3-induced elongation.”
t0“Cucumber Hypocotyl Sections”
Claim · model-written summary
The authors concluded brassinosteroids enhance auxin action independently of gibberellin activity.
- Taxon
- CucumberCucumis sativust0
- Treatment
- brassinosteroidse0
- Outcome
- auxin actione0
- Qualifier
- independent of that of gibberelline0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“brassinosteroids enhance auxin action and possess growth-promoting activity which is independent of that of gibberellin.”
t0“Cucumber Hypocotyl Sections”
The same questions in other papers
indole-3-acetic acid · growth · Cucumis sativus (cucumber)Results differ
11 papers in the same direction as this paper · 4 in the opposite direction
- no effect
- negative
- no effect
12 papers more in this group
See all 16 papers →kinetin · growth · Cucumis sativus (cucumber)Positive and negative results
2 papers in the same direction as this paper · 5 in the opposite direction
- positive
- positive
- positive
4 papers more in this group
See all 8 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.