Abstract
Two studies were conducted to test the effects of various tissue culture media on somatic embryogenesis from cotyledon tissue of cucumber (Cucumis sativus L.). The two best media for embryo initiation were Murashige and Skoog (MS) salts and vitamins containing either 1 or 2 mg 2,4-D/liter and 0.5 mg kinetin/liter. In the second study, embryos developed more normally. More plantlets developed when tissue was removed from the initiation medium after 3 weeks and transferred to MS containing 1 mg NAA/liter and 0.5 mg kinetin/liter for 3 weeks, rather than leaving the embryos on a medium containing 2,4-D. Histological evidence indicated that the embryos were multicellular in origin. Charcoal in the maturation medium inhibited embryo development. Chemical names used: (2,4-dichlorophenoxy) -acetic acid (2,4-D); N-(2-furanylmethyl)-lH-purine-6-amine (kinetin); 1-naphthaleneacetic acid (NAA).
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Claims 5
Claim · model-written summary
Two MS media with either 1 or 2 mg 2,4-D/liter and kinetin were best for embryo initiation.
- Taxon
- cucumber
Cucumis sativust0 - Treatment
- Murashige and Skoog (MS) salts and vitamins containing either 1 or 2 mg 2,4-D/liter and 0.5 mg kinetin/litere0
- Outcome
- embryo initiatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The two best media for embryo initiation were Murashige and Skoog (MS) salts and vitamins containing either 1 or 2 mg 2,4-D/liter and 0.5 mg kinetin/liter.”
t0“cucumber”
Claim · model-written summary
Embryos developed more normally in the second study.
- Taxon
- cucumberCucumis sativust0
- Outcome
- embryos developed more normallye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In the second study, embryos developed more normally.”
t0“Cucumber”
Claim · model-written summary
Transfer to NAA and kinetin medium after three weeks produced more plantlets than continued 2,4-D medium.
- Taxon
- cucumberCucumis sativust0
- Treatment
- transferred to MS containing 1 mg NAA/liter and 0.5 mg kinetin/liter for 3 weekse0
- Outcome
- plantletse0
- Direction
- Moree0
- Comparator
- leaving the embryos on a medium containing 2,4-De0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“More plantlets developed when tissue was removed from the initiation medium after 3 weeks and transferred to MS containing 1 mg NAA/liter and 0.5 mg kinetin/liter for 3 weeks, rather than leaving the embryos on a medium containing 2,4-D.”
t0“Cucumber”
Claim · model-written summary
Histology indicated multicellular embryo origins.
- Taxon
- cucumberCucumis sativust0
- Outcome
- embryos were multicellular in origine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Histological evidence indicated that the embryos were multicellular in origin.”
t0“Cucumber”
Claim · model-written summary
Charcoal in maturation medium inhibited embryo development.
- Taxon
- cucumberCucumis sativust0
- Treatment
- Charcoal in the maturation mediume0
- Outcome
- embryo developmente0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Charcoal in the maturation medium inhibited embryo development.”
t0“Cucumber”
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30 papers more in this group
See all 34 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.