Paper · 1996 · New Phytologist

The genotoxicity of Zn(II) and Cd(II) in Allium cepa root meristematic cells

L. BORBOA; C. DE LA TORRE

11
claims
20
verbatim quotes
56
citations
3
research areas
✓
Open access

Abstract

SUMMARY Applications of 5 × 10 ‐4 m Zn(II) and 3 × 10 ‐5 m Cd(II) depressed but did not block root growth in Allium cepa L. Both heavy metals were indirect clastogens at these moderate concentrations, for they induced the appearance of chromosomal bridges, but no breaks were detected in the first anatelophases. On the other hand, Zn and Cd respectively potentiated six and eleven times the direct clastogenic effect of the β‐irradiation produced by [ 3 H]thymidine incorporation into DNA. The chromosomal breaks at mitosis were more abundant when [ 3 H] was incorporated into early replicating DNA. On the other hand, when the incorporation took place in the last half of interphase, the subsequent G 2 as well as the prophase were extended (1·7‐fold for Zn and 2·7‐fold for Cd), and the Sexcess lesions were repaired before anaphase. The whole cycle experienced 1·5 and 2·0‐fold increases in the presence of Zn and Cd in relation to that in control meristems (15 h).

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Claims 11

Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Zinc depressed but did not block root growth.

Taxon
Allium cepa L.Allium cepae0
Treatment
5 × 10 ‐4 m Zn(II)e0
Outcome
root growthe0
Direction
depressede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Allium cepa
Allium cepa · TaxonMehdi · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Applications of 5 × 10 ‐4 m Zn(II) and 3 × 10 ‐5 m Cd(II) depressed but did not block root growth in Allium cepa L.”

✓ located verbatim in the source · abstract, characters 8–123 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Cadmium depressed but did not block root growth.

Taxon
Allium cepa L.Allium cepae0
Treatment
3 × 10 ‐5 m Cd(II)e0
Outcome
root growthe0
Direction
depressede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Allium cepa
Allium cepa · TaxonMehdi · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Applications of 5 × 10 ‐4 m Zn(II) and 3 × 10 ‐5 m Cd(II) depressed but did not block root growth in Allium cepa L.”

✓ located verbatim in the source · abstract, characters 8–123 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Both metals induced chromosome bridges without detectable first-anatelophase breaks.

Taxon
Allium cepaAllium cepat0
Treatment
Both heavy metalse0
Outcome
chromosomal bridgese0
Qualifier
no breaks were detected in the first anatelophasese0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Allium cepa
Allium cepa · TaxonMehdi · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Both heavy metals were indirect clastogens at these moderate concentrations, for they induced the appearance of chromosomal bridges, but no breaks were detected in the first anatelophases.”

✓ located verbatim in the source · abstract, characters 124–312 · show in text ↑
t0

“Allium cepa”

✓ located verbatim in the source · title, characters 41–52 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Zinc potentiated irradiation clastogenicity six times.

Taxon
Allium cepaAllium cepat0
Treatment
Zne0
Outcome
direct clastogenic effecte0
Direction
potentiatede0
Value
sixe0
Comparator
β‐irradiatione0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Allium cepa
Allium cepa · TaxonMehdi · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Zn and Cd respectively potentiated six and eleven times the direct clastogenic effect of the β‐irradiation produced by [ 3 H]thymidine incorporation into DNA.”

✓ located verbatim in the source · abstract, characters 332–490 · show in text ↑
t0

“Allium cepa”

✓ located verbatim in the source · title, characters 41–52 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Cadmium potentiated irradiation clastogenicity eleven times.

Taxon
Allium cepaAllium cepat0
Treatment
Cde0
Outcome
direct clastogenic effecte0
Direction
potentiatede0
Value
elevene0
Comparator
β‐irradiatione0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Allium cepa
Allium cepa · TaxonMehdi · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Zn and Cd respectively potentiated six and eleven times the direct clastogenic effect of the β‐irradiation produced by [ 3 H]thymidine incorporation into DNA.”

✓ located verbatim in the source · abstract, characters 332–490 · show in text ↑
t0

“Allium cepa”

✓ located verbatim in the source · title, characters 41–52 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

Early replicating DNA incorporation had more mitotic chromosome breaks.

Taxon
Allium cepaAllium cepat0
Treatment
[ 3 H] was incorporated into early replicating DNAe0
Outcome
chromosomal breaks at mitosise0
Direction
more abundante0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Allium cepa
Allium cepa · TaxonMehdi · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The chromosomal breaks at mitosis were more abundant when [ 3 H] was incorporated into early replicating DNA.”

✓ located verbatim in the source · abstract, characters 491–600 · show in text ↑
t0

“Allium cepa”

✓ located verbatim in the source · title, characters 41–52 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Late interphase incorporation extended G2 and prophase 1.7-fold with zinc.

Taxon
Allium cepaAllium cepat0
Treatment
Zne0
Outcome
G 2 as well as the prophasee0
Direction
extendede0
Value
1·7‐folde0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Allium cepa
Allium cepa · TaxonMehdi · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“the subsequent G 2 as well as the prophase were extended (1·7‐fold for Zn and 2·7‐fold for Cd)”

✓ located verbatim in the source · abstract, characters 686–780 · show in text ↑
t0

“Allium cepa”

✓ located verbatim in the source · title, characters 41–52 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Late interphase incorporation extended G2 and prophase 2.7-fold with cadmium.

Taxon
Allium cepaAllium cepat0
Treatment
Cde0
Outcome
G 2 as well as the prophasee0
Direction
extendede0
Value
2·7‐folde0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Allium cepa
Allium cepa · TaxonMehdi · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“the subsequent G 2 as well as the prophase were extended (1·7‐fold for Zn and 2·7‐fold for Cd)”

✓ located verbatim in the source · abstract, characters 686–780 · show in text ↑
t0

“Allium cepa”

✓ located verbatim in the source · title, characters 41–52 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Late incorporation allowed lesions to be repaired before anaphase.

Taxon
Allium cepaAllium cepat0
Outcome
Sexcess lesions were repaired before anaphasee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Allium cepa
Allium cepa · TaxonMehdi · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“the Sexcess lesions were repaired before anaphase.”

✓ located verbatim in the source · abstract, characters 786–836 · show in text ↑
t0

“Allium cepa”

✓ located verbatim in the source · title, characters 41–52 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Zinc lengthened the cell cycle 1.5-fold.

Taxon
Allium cepaAllium cepat0
Treatment
Zne0
Outcome
whole cyclee0
Direction
increasese0
Value
1·5e0
Comparator
control meristemse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Allium cepa
Allium cepa · TaxonMehdi · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The whole cycle experienced 1·5 and 2·0‐fold increases in the presence of Zn and Cd in relation to that in control meristems (15 h).”

✓ located verbatim in the source · abstract, characters 837–969 · show in text ↑
t0

“Allium cepa”

✓ located verbatim in the source · title, characters 41–52 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Cadmium lengthened the cell cycle 2.0-fold.

Taxon
Allium cepaAllium cepat0
Treatment
Cde0
Outcome
whole cyclee0
Direction
increasese0
Value
2·0‐folde0
Comparator
control meristemse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Allium cepa
Allium cepa · TaxonMehdi · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The whole cycle experienced 1·5 and 2·0‐fold increases in the presence of Zn and Cd in relation to that in control meristems (15 h).”

✓ located verbatim in the source · abstract, characters 837–969 · show in text ↑
t0

“Allium cepa”

✓ located verbatim in the source · title, characters 41–52 · show in text ↑

zinc atom · growth · Allium cepa (onion)Results differ

25 papers: 22 positive, 3 negative or no effect

2 papers in the same direction as this paper · 22 in the opposite direction

  1. positive
  2. positive
  3. positive

21 papers more in this group

See all 25 papers →

cadmium atom · growth · Allium cepa (onion)Results agree

7 papers: 0 positive, 7 negative or no effect

6 papers in the same direction as this paper

  1. negative
  2. negative
  3. negative

3 papers more in this group

See all 7 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.