Abstract
The studies about the elucidation of molecular components of drought response in major crops have become an urgent priority. Since abscisic acid (ABA) is one of the major plant hormones influencing main stress responses including drought, cold, and heat, there has been overwhelming interest in investigation of ABA-associated genes. Therefore, the identification and characterization of ABAassociated genes from bread wheat is significant for gaining better insights about the multiple stress-response mechanisms. Due to the pivotal role of ABA in stress response mechanism, we tried to determine spatiotemporal expressional characteristics of ABA-related genes in wheat. The comparative differential transcript profiling experiments revealed a total of 60 differentially-regulated cDNA fragments, thirty of which were sequenced to obtain more details about their functional identities. Some of the sequenced fragments showed significant similarity with the genes known to be associated with ABA-induced signaling networks; e.g., Germin-Like Proteins, Tonoplast Intrinsic Proteins, MAP kinases and leucine-rich repeat (LRR) receptor-like proteins. To get more details about ABAassociated genes in wheat, the expressional characteristics of the transcripts from the initial screening was further investigated by using quantitative Real Time PCR. For example, the amount of transcripts MAPK4 and TIP1 homologs in leaf tissue of bread wheat is gradually increased and eventually peaked in about 2 hours after ABA treatment, and subsequently declined with the prolonged exposure. On the other hand, induction in the GLP homolog expression was much faster than the aforementioned transcripts; reaching to the apex in roughly an hour after the treatment. This study provided insights about the expressional characteristics of some ABA-associated genes from bread wheat. This is the first study investigating the early response genes under ABA-treatment in hexaploid bread wheat.
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Claims 4
Claim · model-written summary
ABA profiling revealed 60 differentially regulated cDNA fragments, 30 of which were sequenced.
- Taxon
- bread wheat
Triticum aestivumt0 - Outcome
- differentially-regulated cDNA fragmentse0
- Value
- 60e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The comparative differential transcript profiling experiments revealed a total of 60 differentially-regulated cDNA fragments, thirty of which were sequenced to obtain more details about their functional identities.”
t0“Abscisic acid regulated gene expression in bread wheat (Triticum aestivum L.)”
Claim · model-written summary
Sequenced fragments resembled genes associated with ABA signaling networks.
- Taxon
- bread wheatTriticum aestivumt0
- Outcome
- significant similaritye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Some of the sequenced fragments showed significant similarity with the genes known to be associated with ABA-induced signaling networks; e.g., Germin-Like Proteins, Tonoplast Intrinsic Proteins, MAP kinases and leucine-rich repeat (LRR) receptor-like proteins.”
t0“Abscisic acid regulated gene expression in bread wheat (Triticum aestivum L.)”
Claim · model-written summary
MAPK4 and TIP1 homolog transcripts rose and peaked about two hours after ABA treatment, then declined.
- Taxon
- bread wheatTriticum aestivume0
- Organ / stage
- leaf tissuee0
- Treatment
- ABA treatmente0
- Outcome
- amount of transcripts MAPK4 and TIP1 homologse0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the amount of transcripts MAPK4 and TIP1 homologs in leaf tissue of bread wheat is gradually increased and eventually peaked in about 2 hours after ABA treatment, and subsequently declined with the prolonged exposure.”
Claim · model-written summary
GLP homolog expression reached its peak roughly one hour after ABA treatment.
- Taxon
- bread wheatTriticum aestivumt0
- Treatment
- the treatmente0
- Outcome
- GLP homolog expressione0
- Direction
- inductione0
- Value
- roughly an houre0
- Comparator
- aforementioned transcriptse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“induction in the GLP homolog expression was much faster than the aforementioned transcripts; reaching to the apex in roughly an hour after the treatment.”
t0“Abscisic acid regulated gene expression in bread wheat (Triticum aestivum L.)”
The same questions in other papers
(+)-abscisic acid · molecular: expression · Triticum aestivum (wheat)Positive and negative results
33 papers in the same direction as this paper · 9 in the opposite direction
- negative
- negative
- no effect
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See all 43 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.