Abstract
Slow anion channels (SLAC/SLAH) are efflux channels previously shown to be critical for stomatal regulation. However, detailed analysis using the β-glucuronidase reporter gene showed that members of the SLAC/SLAH gene family are predominantly expressed in roots, in addition to stomatal guard cells, implicating distinct function(s) of SLAC/SLAH in the roots. Comprehensive mutant analyses of all slac/slah mutants indicated that slah3 plants showed a greater growth defect than wild-type plants when ammonium was supplied as the sole nitrogen source. Ammonium toxicity was mimicked by acidic pH in nitrogen-free external medium, suggesting that medium acidification by ammonium-fed plants may underlie ammonium toxicity. Interestingly, such toxicity was more severe in slah3 mutants and, particularly in wild-type plants, was alleviated by supplementing the media with micromolar levels of nitrate. These data thus provide evidence that SLAH3, a nitrate efflux channel, plays a role in nitrate-dependent alleviation of ammonium toxicity in plants.
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Claims 6
Claim · model-written summary
SLAC/SLAH family members were predominantly expressed in roots as well as stomatal guard cells.
- Taxon
- Arabidopsis
Arabidopsist0 - Organ / stage
- rootse0
- Gene
- SLAC/SLAHe0
- Outcome
- predominantly expressed in roots, in addition to stomatal guard cellse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“members of the SLAC/SLAH gene family are predominantly expressed in roots, in addition to stomatal guard cells”
t0“Arabidopsis”
Claim · model-written summary
With ammonium as sole nitrogen source, slah3 plants had a greater growth defect than wild type.
- Taxon
- ArabidopsisArabidopsist0
- Material
- slah3e0
- Treatment
- ammonium was supplied as the sole nitrogen sourcee0
- Outcome
- growth defecte0
- Direction
- greatere0
- Comparator
- wild-type plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“slah3 plants showed a greater growth defect than wild-type plants when ammonium was supplied as the sole nitrogen source”
t0“Arabidopsis”
Claim · model-written summary
Acidic pH in nitrogen-free medium mimicked ammonium toxicity.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- acidic pHe0
- Outcome
- Ammonium toxicitye0
- Qualifier
- nitrogen-free external mediume0
- Comparator
- Ammonium toxicitye0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ammonium toxicity was mimicked by acidic pH in nitrogen-free external medium”
t0“Arabidopsis”
Claim · model-written summary
Ammonium toxicity was more severe in slah3 mutants.
- Taxon
- ArabidopsisArabidopsist0
- Material
- slah3 mutantse0
- Outcome
- toxicitye0
- Direction
- more severee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“such toxicity was more severe in slah3 mutants”
t0“Arabidopsis”
Claim · model-written summary
Micromolar nitrate alleviated toxicity, particularly in wild-type plants.
- Taxon
- ArabidopsisArabidopsist0
- Material
- wild-type plantse0
- Treatment
- supplementing the media with micromolar levels of nitratee0
- Outcome
- toxicitye1
- Direction
- alleviatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“particularly in wild-type plants, was alleviated by supplementing the media with micromolar levels of nitrate”
e1“such toxicity”
t0“Arabidopsis”
Claim · model-written summary
The authors inferred SLAH3 participates in nitrate-dependent relief of ammonium toxicity.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- SLAH3e0
- Outcome
- nitrate-dependent alleviation of ammonium toxicitye0
- Qualifier
- nitrate efflux channele0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SLAH3, a nitrate efflux channel, plays a role in nitrate-dependent alleviation of ammonium toxicity in plants”
t0“Arabidopsis”
The same questions in other papers
ph · pest · ArabidopsisResults agree
1 paper in the same direction as this paper
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.