Abstract
The TaMATE1B gene (for multidrug and toxic compound extrusion) from wheat (Triticum aestivum) was isolated and shown to encode a citrate transporter that is located on the plasma membrane. TaMATE1B expression in roots was induced by iron deficiency but not by phosphorus deficiency or aluminum treatment. The coding region of TaMATE1B was identical in a genotype showing citrate efflux from root apices (cv Carazinho) to one that lacked citrate efflux (cv Egret). However, sequence upstream of the coding region differed between these two genotypes in two ways. The first difference was a single-nucleotide polymorphism located approximately 2 kb upstream from the start codon in cv Egret. The second difference was an 11.1-kb transposon-like element located 25 bp upstream of the start codon in cv Carazinho that was absent from cv Egret. The influence of these polymorphisms on TaMATE1B expression was investigated using fusions to green fluorescent protein expressed in transgenic lines of rice (Oryza sativa). Fluorescence measurements in roots of rice indicated that 1.5- and 2.3-kb regions upstream of TaMATE1B in cv Carazinho (which incorporated 3' regions of the transposon-like element) generated 20-fold greater expression in the apical 1 mm of root compared with the native promoter in cv Egret. By contrast, fluorescence in more mature tissues was similar in both cultivars. The presence of the single-nucleotide polymorphism alone consistently generated 2-fold greater fluorescence than the cv Egret promoter. We conclude that the transposon-like element in cv Carazinho extends TaMATE1B expression to the root apex, where it confers citrate efflux and enhanced aluminum tolerance.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 11
Claim · model-written summary
Wheat TaMATE1B encodes a plasma-membrane citrate transporter.
- Taxon
- wheat (Triticum aestivum)
Triticum aestivume0 - Gene
- TaMATE1Be0
- Outcome
- encode a citrate transporter that is located on the plasma membranee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The TaMATE1B gene (for multidrug and toxic compound extrusion) from wheat (Triticum aestivum) was isolated and shown to encode a citrate transporter that is located on the plasma membrane.”
Claim · model-written summary
Iron deficiency induced TaMATE1B root expression.
- Taxon
- WheatTriticum aestivumt0
- Treatment
- iron deficiencye0
- Gene
- TaMATE1Be0
- Outcome
- TaMATE1B expression in rootse0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaMATE1B expression in roots was induced by iron deficiency but not by phosphorus deficiency or aluminum treatment.”
t0“Wheat”
Claim · model-written summary
Phosphorus deficiency did not induce TaMATE1B root expression.
- Taxon
- WheatTriticum aestivumt0
- Treatment
- phosphorus deficiencye0
- Gene
- TaMATE1Be0
- Outcome
- TaMATE1B expression in rootse0
- Direction
- note0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaMATE1B expression in roots was induced by iron deficiency but not by phosphorus deficiency or aluminum treatment.”
t0“Wheat”
Claim · model-written summary
Aluminum treatment did not induce TaMATE1B root expression.
- Taxon
- WheatTriticum aestivumt0
- Treatment
- aluminum treatmente0
- Gene
- TaMATE1Be0
- Outcome
- TaMATE1B expression in rootse0
- Direction
- note0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaMATE1B expression in roots was induced by iron deficiency but not by phosphorus deficiency or aluminum treatment.”
t0“Wheat”
Claim · model-written summary
TaMATE1B coding sequence was identical in Carazinho and Egret despite different citrate efflux.
- Taxon
- WheatTriticum aestivumt0
- Material
- cv Carazinhoe0
- Gene
- TaMATE1Be0
- Outcome
- coding region of TaMATE1Be0
- Direction
- identicale0
- Comparator
- cv Egrete0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The coding region of TaMATE1B was identical in a genotype showing citrate efflux from root apices (cv Carazinho) to one that lacked citrate efflux (cv Egret).”
t0“Wheat”
Claim · model-written summary
Egret had a SNP approximately 2 kb upstream of the start codon.
- Taxon
- WheatTriticum aestivumt0
- Material
- cv Egrete0
- Outcome
- single-nucleotide polymorphisme0
- Qualifier
- approximately 2 kb upstream from the start codone0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a single-nucleotide polymorphism located approximately 2 kb upstream from the start codon in cv Egret.”
t0“Wheat”
Claim · model-written summary
Carazinho had an 11.1-kb transposon-like element absent from Egret.
- Taxon
- WheatTriticum aestivumt0
- Material
- cv Carazinhoe0
- Outcome
- transposon-like elemente0
- Value
- 11.1-kbe0
- Qualifier
- 25 bp upstream of the start codone0
- Comparator
- cv Egrete0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“an 11.1-kb transposon-like element located 25 bp upstream of the start codon in cv Carazinho that was absent from cv Egret.”
t0“Wheat”
Claim · model-written summary
Carazinho promoter regions drove 20-fold greater expression in the apical rice root millimeter than Egret promoter.
- Taxon
- rice (Oryza sativa)
Oryza sativat0 - Treatment
- 1.5- and 2.3-kb regions upstream of TaMATE1B in cv Carazinhoe0
- Gene
- TaMATE1Be0
- Outcome
- expression in the apical 1 mm of roote0
- Direction
- greatere0
- Value
- 20-folde0
- Comparator
- native promoter in cv Egrete0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“1.5- and 2.3-kb regions upstream of TaMATE1B in cv Carazinho (which incorporated 3' regions of the transposon-like element) generated 20-fold greater expression in the apical 1 mm of root compared with the native promoter in cv Egret.”
t0“rice (Oryza sativa)”
Claim · model-written summary
Fluorescence was similar in mature tissue for the two cultivars.
- Taxon
- rice (Oryza sativa)Oryza sativat0
- Outcome
- fluorescence in more mature tissuese0
- Direction
- similare0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“fluorescence in more mature tissues was similar in both cultivars.”
t0“rice (Oryza sativa)”
Claim · model-written summary
The SNP alone gave twofold greater fluorescence than the Egret promoter.
- Taxon
- rice (Oryza sativa)Oryza sativat0
- Treatment
- single-nucleotide polymorphisme0
- Outcome
- fluorescencee0
- Direction
- greatere0
- Value
- 2-folde0
- Comparator
- cv Egret promotere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The presence of the single-nucleotide polymorphism alone consistently generated 2-fold greater fluorescence than the cv Egret promoter.”
t0“rice (Oryza sativa)”
Claim · model-written summary
The authors conclude that the Carazinho transposon extends TaMATE1B expression to root apices and confers citrate efflux and aluminum tolerance.
- Taxon
- WheatTriticum aestivumt0
- Material
- cv Carazinhoe0
- Gene
- TaMATE1Be0
- Outcome
- citrate efflux and enhanced aluminum tolerancee0
- Qualifier
- extends TaMATE1B expression to the root apexe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the transposon-like element in cv Carazinho extends TaMATE1B expression to the root apex, where it confers citrate efflux and enhanced aluminum tolerance.”
t0“Wheat”
The same questions in other papers
aluminium atom · molecular: expression · Triticum aestivum (wheat)Positive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- positive
- negative
iron atom · molecular: expression · Triticum aestivum (wheat)Positive and negative results
5 papers in the same direction as this paper · 2 in the opposite direction
This paper is about lacking the nutrient, so it counts the other way round here.
- positive
- positive
- negative
4 papers more in this group
See all 8 papers →phosphorus · molecular: expression · Triticum aestivum (wheat)Positive and negative results
5 papers in the same direction as this paper · 4 in the opposite direction
This paper is about lacking the nutrient, so it counts the other way round here.
- positive
- positive
- positive
6 papers more in this group
See all 10 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.