Abstract
The effect of three growth regulators, namely kinetin, 6 benzyl adenine, 2 chloro ethyl trimethyl ammonium chloride at three concentrations (10-6 M, 5 x 10-5 M 10-4 M) was studied on the catalytic activity of nitrate reductase in green and etiolated seedlings.A concentration of 5 x 10-5 M was optimal for all the growth regulators treatments.All the growth regulators stimulated nitrate reductase activity effectively at 5 x 10-5M concentration in both etiolated and green seedlings and had an additive effect when supplemented by NO-3 up to 140% to 160%.The 99.2% and 93.4% inhibition of nitrate reductase activity resulted in development of etiolated and green seedlings, respectively when treated with eukaryotic 80S ribosome protein synthesis inhibitor cycloheximide.Prokaryotic 70S inhibitor chloromphenicol did not have any effect on measured parameters.Actinomycin D, a RNA synthesis inhibitor also inhibited the enzyme activity as 80s inhibitors (Green 80%, etiolated 98%).One may suggest from this that both DNA and protein synthesis are involved in the induction of nitrate reductase activity.The differential effect of aminoacids was observed on enzyme activity in combination with growth regulators.
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Claims 10
Claim · model-written summary
A concentration of 5 x 10-5 M was optimal for all tested growth regulators.
- Taxon
- RICE
Oryza sativat0
- Treatment
- 5 x 10-5 Me0
- Outcome
- growth regulators treatmentse0
- Direction
- optimale0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A concentration of 5 x 10-5 M was optimal for all the growth regulators treatments.”
t0“RICE (ORYZA SATIVA L.)”
Claim · model-written summary
Growth regulators stimulated nitrate reductase activity in etiolated and green rice seedlings at 5 x 10-5M.
- Taxon
- RICE
Oryza sativat0
- Organ / stage
- etiolated and green seedlingse0
- Treatment
- growth regulatorse0
- Gene
- nitrate reductasee0
- Outcome
- nitrate reductase activitye0
- Direction
- stimulatede0
- Value
- 5 x 10-5Me0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“All the growth regulators stimulated nitrate reductase activity effectively at 5 x 10-5M concentration in both etiolated and green seedlings”
t0“RICE (ORYZA SATIVA L.)”
Claim · model-written summary
Supplementing growth regulators with nitrate produced an additive effect on nitrate reductase activity up to 140% to 160%.
- Taxon
- RICE
Oryza sativat0
- Treatment
- supplemented by NO-3e0
- Outcome
- nitrate reductase activitye1
- Direction
- additive effecte0
- Value
- 140% to 160%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“had an additive effect when supplemented by NO-3 up to 140% to 160%”
e1“stimulated nitrate reductase activity effectively”
t0“RICE (ORYZA SATIVA L.)”
Claim · model-written summary
Cycloheximide inhibited nitrate reductase activity by 99.2% in etiolated rice seedlings.
- Taxon
- RICE
Oryza sativat0
- Organ / stage
- etiolatede0
- Treatment
- cycloheximidee0
- Outcome
- nitrate reductase activitye0
- Direction
- inhibitione0
- Value
- 99.2%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The 99.2% and 93.4% inhibition of nitrate reductase activity resulted in development of etiolated and green seedlings, respectively when treated with eukaryotic 80S ribosome protein synthesis inhibitor cycloheximide.”
t0“RICE (ORYZA SATIVA L.)”
Claim · model-written summary
Cycloheximide inhibited nitrate reductase activity by 93.4% in green rice seedlings.
- Taxon
- RICE
Oryza sativat0
- Organ / stage
- green seedlingse0
- Treatment
- cycloheximidee0
- Outcome
- nitrate reductase activitye0
- Direction
- inhibitione0
- Value
- 93.4%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The 99.2% and 93.4% inhibition of nitrate reductase activity resulted in development of etiolated and green seedlings, respectively when treated with eukaryotic 80S ribosome protein synthesis inhibitor cycloheximide.”
t0“RICE (ORYZA SATIVA L.)”
Claim · model-written summary
Chloromphenicol did not affect the measured parameters in rice seedlings.
- Taxon
- RICE
Oryza sativat0
- Treatment
- chloromphenicole0
- Outcome
- measured parameterse0
- Direction
- did not have any effecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Prokaryotic 70S inhibitor chloromphenicol did not have any effect on measured parameters.”
t0“RICE (ORYZA SATIVA L.)”
Claim · model-written summary
Actinomycin D inhibited nitrate reductase activity by 80% in green rice seedlings.
- Taxon
- RICE
Oryza sativat0
- Organ / stage
- Greene0
- Treatment
- Actinomycin De0
- Outcome
- enzyme activitye0
- Direction
- inhibitede0
- Value
- 80%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Actinomycin D, a RNA synthesis inhibitor also inhibited the enzyme activity as 80s inhibitors (Green 80%, etiolated 98%).”
t0“RICE (ORYZA SATIVA L.)”
Claim · model-written summary
Actinomycin D inhibited nitrate reductase activity by 98% in etiolated rice seedlings.
- Taxon
- RICE
Oryza sativat0
- Organ / stage
- etiolatede0
- Treatment
- Actinomycin De0
- Outcome
- enzyme activitye0
- Direction
- inhibitede0
- Value
- 98%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Actinomycin D, a RNA synthesis inhibitor also inhibited the enzyme activity as 80s inhibitors (Green 80%, etiolated 98%).”
t0“RICE (ORYZA SATIVA L.)”
Claim · model-written summary
The authors suggest DNA and protein synthesis are involved in induction of nitrate reductase activity.
- Taxon
- RICE
Oryza sativat0
- Outcome
- induction of nitrate reductase activitye0
- Qualifier
- DNA and protein synthesise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“both DNA and protein synthesis are involved in the induction of nitrate reductase activity”
t0“RICE (ORYZA SATIVA L.)”
Claim · model-written summary
Amino acids had differential effects on enzyme activity in combination with growth regulators.
- Taxon
- RICE
Oryza sativat0
- Treatment
- aminoacidse0
- Outcome
- enzyme activitye0
- Direction
- differential effecte0
- Qualifier
- in combination with growth regulatorse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The differential effect of aminoacids was observed on enzyme activity in combination with growth regulators.”
t0“RICE (ORYZA SATIVA L.)”
The same questions in other papers
nitric oxide · physiology: enzyme activity · Oryza sativa (rice)Results agree
1 paper in the same direction as this paper
- positive
cycloheximide · physiology: enzyme activity · Oryza sativa (rice)Results agree
2 papers in the same direction as this paper
- negative
- negative
class · physiology: enzyme activity · Oryza sativa (rice)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.