Paper · 2002 · University of Zagreb University Computing Centre (SRCE)

REGULATION OF NITRATE REDUCTASE ACTIVITY IN RICE (ORYZA SATIVA L.) BY GROWTH REGULATORS

HEMALATHA, S.

10
claims
21
verbatim quotes
9
citations
1
research areas
✓
Open access · other-oa

Abstract

The effect of three growth regulators, namely kinetin, 6 benzyl adenine, 2 chloro ethyl trimethyl ammonium chloride at three concentrations (10-6 M, 5 x 10-5 M 10-4 M) was studied on the catalytic activity of nitrate reductase in green and etiolated seedlings.A concentration of 5 x 10-5 M was optimal for all the growth regulators treatments.All the growth regulators stimulated nitrate reductase activity effectively at 5 x 10-5M concentration in both etiolated and green seedlings and had an additive effect when supplemented by NO-3 up to 140% to 160%.The 99.2% and 93.4% inhibition of nitrate reductase activity resulted in development of etiolated and green seedlings, respectively when treated with eukaryotic 80S ribosome protein synthesis inhibitor cycloheximide.Prokaryotic 70S inhibitor chloromphenicol did not have any effect on measured parameters.Actinomycin D, a RNA synthesis inhibitor also inhibited the enzyme activity as 80s inhibitors (Green 80%, etiolated 98%).One may suggest from this that both DNA and protein synthesis are involved in the induction of nitrate reductase activity.The differential effect of aminoacids was observed on enzyme activity in combination with growth regulators.

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Claims 10

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

A concentration of 5 x 10-5 M was optimal for all tested growth regulators.

Treatment
5 x 10-5 Me0
Outcome
growth regulators treatmentse0
Direction
optimale0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“A concentration of 5 x 10-5 M was optimal for all the growth regulators treatments.”

✓ located verbatim in the source · abstract, characters 260–343 · show in text ↑
t0

“RICE (ORYZA SATIVA L.)”

✓ located verbatim in the source · title, characters 44–66 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Growth regulators stimulated nitrate reductase activity in etiolated and green rice seedlings at 5 x 10-5M.

Organ / stage
etiolated and green seedlingse0
Treatment
growth regulatorse0
Gene
nitrate reductasee0
Outcome
nitrate reductase activitye0
Direction
stimulatede0
Value
5 x 10-5Me0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“All the growth regulators stimulated nitrate reductase activity effectively at 5 x 10-5M concentration in both etiolated and green seedlings”

✓ located verbatim in the source · abstract, characters 343–483 · show in text ↑
t0

“RICE (ORYZA SATIVA L.)”

✓ located verbatim in the source · title, characters 44–66 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Supplementing growth regulators with nitrate produced an additive effect on nitrate reductase activity up to 140% to 160%.

Treatment
supplemented by NO-3e0
Outcome
nitrate reductase activitye1
Direction
additive effecte0
Value
140% to 160%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“had an additive effect when supplemented by NO-3 up to 140% to 160%”

✓ located verbatim in the source · abstract, characters 488–555 · show in text ↑
e1

“stimulated nitrate reductase activity effectively”

✓ located verbatim in the source · abstract, characters 369–418 · show in text ↑
t0

“RICE (ORYZA SATIVA L.)”

✓ located verbatim in the source · title, characters 44–66 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Cycloheximide inhibited nitrate reductase activity by 99.2% in etiolated rice seedlings.

Organ / stage
etiolatede0
Treatment
cycloheximidee0
Outcome
nitrate reductase activitye0
Direction
inhibitione0
Value
99.2%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“The 99.2% and 93.4% inhibition of nitrate reductase activity resulted in development of etiolated and green seedlings, respectively when treated with eukaryotic 80S ribosome protein synthesis inhibitor cycloheximide.”

✓ located verbatim in the source · abstract, characters 556–772 · show in text ↑
t0

“RICE (ORYZA SATIVA L.)”

✓ located verbatim in the source · title, characters 44–66 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Cycloheximide inhibited nitrate reductase activity by 93.4% in green rice seedlings.

Organ / stage
green seedlingse0
Treatment
cycloheximidee0
Outcome
nitrate reductase activitye0
Direction
inhibitione0
Value
93.4%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“The 99.2% and 93.4% inhibition of nitrate reductase activity resulted in development of etiolated and green seedlings, respectively when treated with eukaryotic 80S ribosome protein synthesis inhibitor cycloheximide.”

✓ located verbatim in the source · abstract, characters 556–772 · show in text ↑
t0

“RICE (ORYZA SATIVA L.)”

✓ located verbatim in the source · title, characters 44–66 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Chloromphenicol did not affect the measured parameters in rice seedlings.

Treatment
chloromphenicole0
Outcome
measured parameterse0
Direction
did not have any effecte0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“Prokaryotic 70S inhibitor chloromphenicol did not have any effect on measured parameters.”

✓ located verbatim in the source · abstract, characters 772–861 · show in text ↑
t0

“RICE (ORYZA SATIVA L.)”

✓ located verbatim in the source · title, characters 44–66 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Actinomycin D inhibited nitrate reductase activity by 80% in green rice seedlings.

Organ / stage
Greene0
Treatment
Actinomycin De0
Outcome
enzyme activitye0
Direction
inhibitede0
Value
80%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“Actinomycin D, a RNA synthesis inhibitor also inhibited the enzyme activity as 80s inhibitors (Green 80%, etiolated 98%).”

✓ located verbatim in the source · abstract, characters 861–982 · show in text ↑
t0

“RICE (ORYZA SATIVA L.)”

✓ located verbatim in the source · title, characters 44–66 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Actinomycin D inhibited nitrate reductase activity by 98% in etiolated rice seedlings.

Organ / stage
etiolatede0
Treatment
Actinomycin De0
Outcome
enzyme activitye0
Direction
inhibitede0
Value
98%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“Actinomycin D, a RNA synthesis inhibitor also inhibited the enzyme activity as 80s inhibitors (Green 80%, etiolated 98%).”

✓ located verbatim in the source · abstract, characters 861–982 · show in text ↑
t0

“RICE (ORYZA SATIVA L.)”

✓ located verbatim in the source · title, characters 44–66 · show in text ↑
Mechanismauthor interpretationoriginal resultmeasured

Claim · model-written summary

The authors suggest DNA and protein synthesis are involved in induction of nitrate reductase activity.

Outcome
induction of nitrate reductase activitye0
Qualifier
DNA and protein synthesise0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“both DNA and protein synthesis are involved in the induction of nitrate reductase activity”

✓ located verbatim in the source · abstract, characters 1013–1103 · show in text ↑
t0

“RICE (ORYZA SATIVA L.)”

✓ located verbatim in the source · title, characters 44–66 · show in text ↑
Intervention effectobservedoriginal resultmixedmeasured

Claim · model-written summary

Amino acids had differential effects on enzyme activity in combination with growth regulators.

Treatment
aminoacidse0
Outcome
enzyme activitye0
Direction
differential effecte0
Qualifier
in combination with growth regulatorse0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“The differential effect of aminoacids was observed on enzyme activity in combination with growth regulators.”

✓ located verbatim in the source · abstract, characters 1104–1212 · show in text ↑
t0

“RICE (ORYZA SATIVA L.)”

✓ located verbatim in the source · title, characters 44–66 · show in text ↑

nitric oxide · physiology: enzyme activity · Oryza sativa (rice)Results agree

2 papers: 2 positive, 0 negative or no effect

1 paper in the same direction as this paper

  1. positive
See all 2 papers →

cycloheximide · physiology: enzyme activity · Oryza sativa (rice)Results agree

3 papers: 0 positive, 3 negative or no effect

2 papers in the same direction as this paper

  1. negative
  2. negative
See all 3 papers →

class · physiology: enzyme activity · Oryza sativa (rice)Results agree

4 papers: 4 positive, 0 negative or no effect

3 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive
See all 4 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.