Abstract
Rice plants(Oryza sativa L.japonica ssp.cv.Nipponbare) were cultivated with ammonium(NH+4-N) or nitrate(NO-3-N) as sole nitrogen in a hydroponics experiment,and the plasma membrane vesicles of leaves were isolated by two-phase system at the seedling stage.The plasma membrane H+-ATPase hydrolytic activity and proton pump activity were analyzed for elucidating the responses of the plasma membrane H+-ATPase of rice leaves to ammonium or nitrate nutrition.The purity of plasma membrane is above 80%.The optimum pH for the plasma membrane H+-ATPase is 6.2 under both NH+4-N and NO-3-N cultivated plants.The plasma membrane H+-ATPase hydrolytic activity,Vmax and Km obtained from the NO-3-N fed rice leaves are significantly higher than those from the NH+4-N fed rice leaves. In addition,the Western Blot showed that the enzyme concentration of plasma membrane H+-ATPase from rice leaves of NO-3-N fed plants was higher than that of NH+4-N fed plants.These results indicate that the higher activity of H+-ATPase from rice leaves of NO-3-N fed plants is resulted from the increased number of H+-ATPase units per membrane area and up-regulated expression of H+-ATPase.The H+-pump activity of the plasma membrane from NO-3-N fed rice leaves is larger than that of NH+4-N fed rice leaves.As the NO-3-transport across the plasma membrane is an active transport process in relation to the plasma membrane H+-ATPase,it might be true that higher activity of plasma membrane H+-ATPase of rice leaves with NO-3-N nutrition is induced by uptake of large amount NO-3-N in to the leaf cells.
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Claims 9
Claim · model-written summary
Leaf plasma membrane preparations had purity above 80%.
- Taxon
- rice
Oryza sativat0 - Outcome
- purity of plasma membranee0
- Value
- above 80%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice leaves”
e0“The purity of plasma membrane is above 80%”
Claim · model-written summary
The H+-ATPase optimum pH was 6.2 under both nitrogen forms.
- Taxon
- riceOryza sativat0
- Gene
- plasma membrane H+-ATPasee0
- Outcome
- optimum pHe0
- Value
- 6.2e0
- Qualifier
- both NH+4-N and NO-3-N cultivated plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice leaves”
e0“The optimum pH for the plasma membrane H+-ATPase is 6.2 under both NH+4-N and NO-3-N cultivated plants”
Claim · model-written summary
Nitrate-fed rice had significantly higher H+-ATPase hydrolytic activity than ammonium-fed rice.
- Taxon
- riceOryza sativae0
- Treatment
- NO-3-Ne0
- Gene
- H+-ATPasee0
- Outcome
- plasma membrane H+-ATPase hydrolytic activitye0
- Direction
- highere0
- Comparator
- NH+4-Ne0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The plasma membrane H+-ATPase hydrolytic activity,Vmax and Km obtained from the NO-3-N fed rice leaves are significantly higher than those from the NH+4-N fed rice leaves”
Claim · model-written summary
Nitrate-fed rice had significantly higher H+-ATPase Vmax than ammonium-fed rice.
- Taxon
- riceOryza sativae0
- Treatment
- NO-3-Ne0
- Gene
- H+-ATPasee0
- Outcome
- Vmaxe0
- Direction
- highere0
- Comparator
- NH+4-Ne0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The plasma membrane H+-ATPase hydrolytic activity,Vmax and Km obtained from the NO-3-N fed rice leaves are significantly higher than those from the NH+4-N fed rice leaves”
Claim · model-written summary
Nitrate-fed rice had significantly higher H+-ATPase Km than ammonium-fed rice.
- Taxon
- riceOryza sativae0
- Treatment
- NO-3-Ne0
- Gene
- H+-ATPasee0
- Outcome
- Kme0
- Direction
- highere0
- Comparator
- NH+4-Ne0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The plasma membrane H+-ATPase hydrolytic activity,Vmax and Km obtained from the NO-3-N fed rice leaves are significantly higher than those from the NH+4-N fed rice leaves”
Claim · model-written summary
Nitrate-fed rice had higher leaf plasma membrane H+-ATPase concentration.
- Taxon
- riceOryza sativae0
- Treatment
- NO-3-Ne0
- Gene
- H+-ATPasee0
- Outcome
- enzyme concentration of plasma membrane H+-ATPasee0
- Direction
- highere0
- Comparator
- NH+4-Ne0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the enzyme concentration of plasma membrane H+-ATPase from rice leaves of NO-3-N fed plants was higher than that of NH+4-N fed plants”
Claim · model-written summary
The authors attribute higher H+-ATPase activity under nitrate to more enzyme units and up-regulated expression.
- Taxon
- riceOryza sativae0
- Treatment
- NO-3-Ne0
- Gene
- H+-ATPasee0
- Outcome
- higher activity of H+-ATPasee0
- Qualifier
- increased number of H+-ATPase units per membrane area and up-regulated expression of H+-ATPasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the higher activity of H+-ATPase from rice leaves of NO-3-N fed plants is resulted from the increased number of H+-ATPase units per membrane area and up-regulated expression of H+-ATPase”
Claim · model-written summary
Nitrate-fed rice had greater plasma membrane proton-pump activity than ammonium-fed rice.
- Taxon
- riceOryza sativae0
- Treatment
- NO-3-Ne0
- Outcome
- H+-pump activity of the plasma membranee0
- Direction
- largere0
- Comparator
- NH+4-Ne0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The H+-pump activity of the plasma membrane from NO-3-N fed rice leaves is larger than that of NH+4-N fed rice leaves”
Claim · model-written summary
The authors hypothesize that nitrate uptake into leaf cells induces higher H+-ATPase activity.
- Taxon
- riceOryza sativae0
- Treatment
- NO-3-N nutritione0
- Gene
- H+-ATPasee0
- Outcome
- higher activity of plasma membrane H+-ATPasee0
- Qualifier
- uptake of large amount NO-3-N in to the leaf cellse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“higher activity of plasma membrane H+-ATPase of rice leaves with NO-3-N nutrition is induced by uptake of large amount NO-3-N in to the leaf cells”
The same questions in other papers
nitric oxide · physiology: enzyme activity · Oryza sativa (rice)Results agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.