Abstract
The embryogenic capability of five soybean cultivars (Renascença, IAS-5, IAC-17, BR-16 and FT-Cometa) was studied at different auxin concentrations (8, 10 and 12 mg/l naphthalene acetic acid, NAA), at different pHs (5.8 and 7.0) and at low (8-12 muEm-2 s-1) and high (27-33 mEm-2 s-1) light intensities. The experimental design was completely randomized with four replications. Immature cotyledons 4-6 mm in length were placed in the six induction mediums evaluated and submitted to two light intensities. Twenty immature cotyledons per cultivar were placed on each Petri dish, which was considered to be one replication. The number of somatic embryos per treatment per replication was counted. The results showed genotype influence on somatic embryogenic capability of each cultivar, with the most embryogenic cultivars being BR-16, FT-Cometa and IAS-5. Auxin concentration and pH value also influenced somatic embryo production, with 10 mg/l NAA being the best auxin concentration and 7.0 the best pH value. The interactions cultivar x auxin, auxin x pH and pH x light were significant, while other double interactions were not. All triple and quadruple interactions were significant, except cultivar x pH x light. No significant differences in somatic embryo production were observed in medium with different pHs or when the Petri dishes containing immature cotyledons were exposed to the two light intensities evaluated. However, a higher number of somatic embryos was produced when the medium pH was adjusted to 7.0.
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Claims 8
Claim · model-written summary
Genotype influenced somatic embryogenic capability of soybean cultivars, with BR-16, FT-Cometa and IAS-5 the most embryogenic.
- Taxon
- soybean
Glycine maxt0 - Material
- BR-16, FT-Cometa and IAS-5e0
- Organ / stage
- Immature cotyledonse1
- Outcome
- somatic embryogenic capabilitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“The results showed genotype influence on somatic embryogenic capability of each cultivar, with the most embryogenic cultivars being BR-16, FT-Cometa and IAS-5”
e1“Immature cotyledons 4-6 mm in length”
t0“soybean”
Claim · model-written summary
Auxin concentration influenced somatic embryo production in soybean, with 10 mg/l NAA the best auxin concentration.
- Taxon
- soybean
Glycine maxt0 - Organ / stage
- Immature cotyledonse1
- Treatment
- 10 mg/l NAAe0
- Outcome
- somatic embryo productione0
- Direction
- beste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“Auxin concentration and pH value also influenced somatic embryo production, with 10 mg/l NAA being the best auxin concentration”
e1“Immature cotyledons 4-6 mm in length”
t0“soybean”
Claim · model-written summary
Medium pH adjusted to 7.0 gave a higher number of somatic embryos than pH 5.8 in soybean.
- Taxon
- soybean
Glycine maxt0 - Organ / stage
- Immature cotyledonse2
- Treatment
- the medium pH was adjusted to 7.0e0
- Outcome
- number of somatic embryose0
- Direction
- highere0
- Comparator
- 5.8e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“However, a higher number of somatic embryos was produced when the medium pH was adjusted to 7.0”
e1“at different pHs (5.8 and 7.0)”
e2“Immature cotyledons 4-6 mm in length”
t0“soybean”
Claim · model-written summary
No significant differences in somatic embryo production were observed between media with different pHs.
- Taxon
- soybean
Glycine maxt0 - Organ / stage
- Immature cotyledonse1
- Treatment
- different pHse0
- Outcome
- somatic embryo productione0
- Direction
- No significant differencese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“No significant differences in somatic embryo production were observed in medium with different pHs”
e1“Immature cotyledons 4-6 mm in length”
t0“soybean”
Claim · model-written summary
No significant differences in somatic embryo production were observed when Petri dishes were exposed to the two light intensities evaluated.
- Taxon
- soybean
Glycine maxt0 - Organ / stage
- Immature cotyledonse1
- Treatment
- two light intensitiese0
- Outcome
- somatic embryo productione0
- Direction
- No significant differencese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“No significant differences in somatic embryo production were observed in medium with different pHs or when the Petri dishes containing immature cotyledons were exposed to the two light intensities evaluated”
e1“Immature cotyledons 4-6 mm in length”
t0“soybean”
Claim · model-written summary
Cultivar x auxin, auxin x pH and pH x light interactions were significant for somatic embryo production.
- Taxon
- soybean
Glycine maxt0 - Outcome
- The interactions cultivar x auxin, auxin x pH and pH x lighte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“The interactions cultivar x auxin, auxin x pH and pH x light were significant, while other double interactions were not”
t0“soybean”
Claim · model-written summary
Other double interactions were not significant.
- Taxon
- soybean
Glycine maxt0 - Outcome
- other double interactionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“while other double interactions were not”
t0“soybean”
Claim · model-written summary
All triple and quadruple interactions were significant, except cultivar x pH x light.
- Taxon
- soybean
Glycine maxt0 - Outcome
- All triple and quadruple interactionse0
- Qualifier
- except cultivar x pH x lighte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“All triple and quadruple interactions were significant, except cultivar x pH x light”
t0“soybean”
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