Paper · 2000 · Genetics and Molecular Biology

Induction of somatic embryogenesis in soybean: physicochemical factors influencing the development of somatic embryos

Gisele Aparecida Bonacin; Antonio Orlando Di Mauro; Roberto Carlos de Oliveira; et al.

8
claims
22
verbatim quotes
10
citations
2
research areas
✓
Open access · cc-by

Abstract

The embryogenic capability of five soybean cultivars (Renascença, IAS-5, IAC-17, BR-16 and FT-Cometa) was studied at different auxin concentrations (8, 10 and 12 mg/l naphthalene acetic acid, NAA), at different pHs (5.8 and 7.0) and at low (8-12 muEm-2 s-1) and high (27-33 mEm-2 s-1) light intensities. The experimental design was completely randomized with four replications. Immature cotyledons 4-6 mm in length were placed in the six induction mediums evaluated and submitted to two light intensities. Twenty immature cotyledons per cultivar were placed on each Petri dish, which was considered to be one replication. The number of somatic embryos per treatment per replication was counted. The results showed genotype influence on somatic embryogenic capability of each cultivar, with the most embryogenic cultivars being BR-16, FT-Cometa and IAS-5. Auxin concentration and pH value also influenced somatic embryo production, with 10 mg/l NAA being the best auxin concentration and 7.0 the best pH value. The interactions cultivar x auxin, auxin x pH and pH x light were significant, while other double interactions were not. All triple and quadruple interactions were significant, except cultivar x pH x light. No significant differences in somatic embryo production were observed in medium with different pHs or when the Petri dishes containing immature cotyledons were exposed to the two light intensities evaluated. However, a higher number of somatic embryos was produced when the medium pH was adjusted to 7.0.

Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 8

Observationobservedoriginal resultmeasured

Claim · model-written summary

Genotype influenced somatic embryogenic capability of soybean cultivars, with BR-16, FT-Cometa and IAS-5 the most embryogenic.

Taxon
soybeanGlycine maxt0
Material
BR-16, FT-Cometa and IAS-5e0
Organ / stage
Immature cotyledonse1
Outcome
somatic embryogenic capabilitye0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Glycine max
Glycine max · TaxonPublic domain · Wikimedia Commons ↗

Verbatim

e0

“The results showed genotype influence on somatic embryogenic capability of each cultivar, with the most embryogenic cultivars being BR-16, FT-Cometa and IAS-5”

✓ located verbatim in the source · abstract, characters 695–853 · show in text ↑
e1

“Immature cotyledons 4-6 mm in length”

✓ located verbatim in the source · abstract, characters 378–414 · show in text ↑
t0

“soybean”

✓ located verbatim in the source · title, characters 38–45 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Auxin concentration influenced somatic embryo production in soybean, with 10 mg/l NAA the best auxin concentration.

Taxon
soybeanGlycine maxt0
Organ / stage
Immature cotyledonse1
Treatment
10 mg/l NAAe0
Outcome
somatic embryo productione0
Direction
beste0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Glycine max
Glycine max · TaxonPublic domain · Wikimedia Commons ↗

Verbatim

e0

“Auxin concentration and pH value also influenced somatic embryo production, with 10 mg/l NAA being the best auxin concentration”

✓ located verbatim in the source · abstract, characters 855–982 · show in text ↑
e1

“Immature cotyledons 4-6 mm in length”

✓ located verbatim in the source · abstract, characters 378–414 · show in text ↑
t0

“soybean”

✓ located verbatim in the source · title, characters 38–45 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Medium pH adjusted to 7.0 gave a higher number of somatic embryos than pH 5.8 in soybean.

Taxon
soybeanGlycine maxt0
Organ / stage
Immature cotyledonse2
Treatment
the medium pH was adjusted to 7.0e0
Outcome
number of somatic embryose0
Direction
highere0
Comparator
5.8e1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Glycine max
Glycine max · TaxonPublic domain · Wikimedia Commons ↗

Verbatim

e0

“However, a higher number of somatic embryos was produced when the medium pH was adjusted to 7.0”

✓ located verbatim in the source · abstract, characters 1425–1520 · show in text ↑
e1

“at different pHs (5.8 and 7.0)”

✓ located verbatim in the source · abstract, characters 198–228 · show in text ↑
e2

“Immature cotyledons 4-6 mm in length”

✓ located verbatim in the source · abstract, characters 378–414 · show in text ↑
t0

“soybean”

✓ located verbatim in the source · title, characters 38–45 · show in text ↑
Intervention effectobservedoriginal resultno effectnot significant (as stated)measured

Claim · model-written summary

No significant differences in somatic embryo production were observed between media with different pHs.

Taxon
soybeanGlycine maxt0
Organ / stage
Immature cotyledonse1
Treatment
different pHse0
Outcome
somatic embryo productione0
Direction
No significant differencese0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Glycine max
Glycine max · TaxonPublic domain · Wikimedia Commons ↗

Verbatim

e0

“No significant differences in somatic embryo production were observed in medium with different pHs”

✓ located verbatim in the source · abstract, characters 1217–1315 · show in text ↑
e1

“Immature cotyledons 4-6 mm in length”

✓ located verbatim in the source · abstract, characters 378–414 · show in text ↑
t0

“soybean”

✓ located verbatim in the source · title, characters 38–45 · show in text ↑
Intervention effectobservedoriginal resultno effectnot significant (as stated)measured

Claim · model-written summary

No significant differences in somatic embryo production were observed when Petri dishes were exposed to the two light intensities evaluated.

Taxon
soybeanGlycine maxt0
Organ / stage
Immature cotyledonse1
Treatment
two light intensitiese0
Outcome
somatic embryo productione0
Direction
No significant differencese0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Glycine max
Glycine max · TaxonPublic domain · Wikimedia Commons ↗

Verbatim

e0

“No significant differences in somatic embryo production were observed in medium with different pHs or when the Petri dishes containing immature cotyledons were exposed to the two light intensities evaluated”

✓ located verbatim in the source · abstract, characters 1217–1423 · show in text ↑
e1

“Immature cotyledons 4-6 mm in length”

✓ located verbatim in the source · abstract, characters 378–414 · show in text ↑
t0

“soybean”

✓ located verbatim in the source · title, characters 38–45 · show in text ↑
Observationobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

Cultivar x auxin, auxin x pH and pH x light interactions were significant for somatic embryo production.

Taxon
soybeanGlycine maxt0
Outcome
The interactions cultivar x auxin, auxin x pH and pH x lighte0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Glycine max
Glycine max · TaxonPublic domain · Wikimedia Commons ↗

Verbatim

e0

“The interactions cultivar x auxin, auxin x pH and pH x light were significant, while other double interactions were not”

✓ located verbatim in the source · abstract, characters 1010–1129 · show in text ↑
t0

“soybean”

✓ located verbatim in the source · title, characters 38–45 · show in text ↑
Observationobservedoriginal resultnot significant (as stated)measured

Claim · model-written summary

Other double interactions were not significant.

Taxon
soybeanGlycine maxt0
Outcome
other double interactionse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Glycine max
Glycine max · TaxonPublic domain · Wikimedia Commons ↗

Verbatim

e0

“while other double interactions were not”

✓ located verbatim in the source · abstract, characters 1089–1129 · show in text ↑
t0

“soybean”

✓ located verbatim in the source · title, characters 38–45 · show in text ↑
Observationobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

All triple and quadruple interactions were significant, except cultivar x pH x light.

Taxon
soybeanGlycine maxt0
Outcome
All triple and quadruple interactionse0
Qualifier
except cultivar x pH x lighte0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Glycine max
Glycine max · TaxonPublic domain · Wikimedia Commons ↗

Verbatim

e0

“All triple and quadruple interactions were significant, except cultivar x pH x light”

✓ located verbatim in the source · abstract, characters 1131–1215 · show in text ↑
t0

“soybean”

✓ located verbatim in the source · title, characters 38–45 · show in text ↑

1-naphthaleneacetic acid · yield · Glycine max (soybean)Results agree

11 papers: 11 positive, 0 negative or no effect

10 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive

7 papers more in this group

See all 11 papers →

ph · growth · Glycine max (soybean)Positive and negative results

34 papers: 10 positive, 24 negative or no effect

9 papers in the same direction as this paper · 24 in the opposite direction

  1. negative
  2. negative
  3. negative

30 papers more in this group

See all 34 papers →

in vitro · growth · Glycine max (soybean)Positive and negative results

53 papers: 38 positive, 15 negative or no effect

37 papers in the same direction as this paper · 14 in the opposite direction · 1 reporting no difference from an alternative

  1. positive
  2. negative
  3. negative

49 papers more in this group

See all 53 papers →

light · yield · Glycine max (soybean)Positive and negative results

71 papers: 50 positive, 21 negative or no effect

20 papers in the same direction as this paper · 50 in the opposite direction

  1. positive
  2. positive
  3. positive

67 papers more in this group

See all 71 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.