Abstract
Biological control of plant diseases by the application of antagonistic micro-organisms to the plant phyllosphere is only marginally understood. Suppression subtractive hybridization (SSH) was used for the identification of genes expressed after application of the non-pathogenic bacterium Pseudomonas fluorescens Bk3 to the phyllosphere of the apple scab-susceptible cultivar Malus domestica cv. Holsteiner Cox. In total, 157 expressed sequence tag (EST) clones were obtained. The sequencing of 113 ESTs which have a significantly elevated transcript level and the comparison of the obtained sequences with databases revealed similarities to different classes of pathogenesis-related proteins, for example, RNase-like PR10 protein and endochitinase, or similarities to proteins expressed under stress conditions that could have a protective function, for example, a germin-like protein, glutathione S-transferase, thioredoxin-like proteins, and heat shock proteins. In addition, several transcripts were identified that code for proteins which have a crucial role at different stages of pathogen recognition and in signalling pathways or an as yet unknown function in plant defence. The results show that a number of transcripts encoding proteins/enzymes which are known to be up-regulated after pathogen infection are also up-regulated after the application of a non-pathogenic bacterium to a M. domestica cultivar. The expression of these proteins might increase the plant resistance towards pathogen infection and damage.
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Claims 5
Claim · model-written summary
The analysis obtained 157 expressed sequence tag clones.
- Taxon
- Malus domestica
Malus domesticat0 - Outcome
- expressed sequence tag (EST) clonese0
- Value
- 157e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In total, 157 expressed sequence tag (EST) clones were obtained.”
t0“Malus domestica”
Claim · model-written summary
Sequencing 113 elevated transcripts identified similarities to pathogenesis-related and stress-associated proteins.
- Taxon
- Malus domesticaMalus domesticat0
- Gene
- RNase-like PR10 protein and endochitinasee0
- Outcome
- similarities to different classes of pathogenesis-related proteinse0
- Value
- 113 ESTse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The sequencing of 113 ESTs which have a significantly elevated transcript level and the comparison of the obtained sequences with databases revealed similarities to different classes of pathogenesis-related proteins, for example, RNase-like PR10 protein and endochitinase, or similarities to proteins expressed under stress conditions that could have a protective function, for example, a germin-like protein, glutathione S-transferase, thioredoxin-like proteins, and heat shock proteins.”
t0“Malus domestica”
Claim · model-written summary
Several identified transcripts encode proteins involved in pathogen recognition and signalling, or with unknown plant defence function.
- Taxon
- Malus domesticaMalus domesticat0
- Outcome
- transcripts were identified that code for proteinse0
- Qualifier
- pathogen recognition and in signalling pathways or an as yet unknown function in plant defencee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“several transcripts were identified that code for proteins which have a crucial role at different stages of pathogen recognition and in signalling pathways or an as yet unknown function in plant defence.”
t0“Malus domestica”
Claim · model-written summary
Applying Pseudomonas fluorescens Bk3 up-regulated apple transcripts also known to be induced after pathogen infection.
- Taxon
- Malus domesticaMalus domesticae1
- Material
- Malus domestica cv. Holsteiner Coxe1
- Treatment
- application of the non-pathogenic bacterium Pseudomonas fluorescens Bk3e1
- Interactor
- Pseudomonas fluorescens Bk3
.JPG?width=60)
Pseudomonas fluorescense1biocontrol_agent - Outcome
- transcripts encoding proteins/enzymese0
- Direction
- up-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.JPG?width=500)
Verbatim
e0“a number of transcripts encoding proteins/enzymes which are known to be up-regulated after pathogen infection are also up-regulated after the application of a non-pathogenic bacterium to a M. domestica cultivar.”
e1“application of the non-pathogenic bacterium Pseudomonas fluorescens Bk3 to the phyllosphere of the apple scab-susceptible cultivar Malus domestica cv. Holsteiner Cox.”
Claim · model-written summary
The authors suggest that expression of these proteins might increase apple resistance to pathogen damage.
- Taxon
- Malus domesticaMalus domesticat0
- Outcome
- plant resistance towards pathogen infection and damagee0
- Qualifier
- expression of these proteinse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The expression of these proteins might increase the plant resistance towards pathogen infection and damage.”
t0“Malus domestica”
The same questions in other papers
Pseudomonas fluorescens · molecular: expression · Malus domestica (apple)Results agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.