Abstract
The biological control of plant diseases by application of antagonistic microorganisms to the plant phyllosphere is an alternative strategy to prevent the frequent treatment of plants by pesticides. Microbiological antagonists can firstly interact directly against the pathogen by releasing antimicrobial compounds and/or secondly induce the plant resistance of the host plant by expression of pathogenesis-related proteins (PR proteins). The focus of our study is on the interaction of the non-pathogenic bacterium Pseudomonas fluorescens Bk3 to the plant phyllosphere of Malus domestica cv. Holsteiner Cox. After application of P. fluorescens Bk3 to the phyllosphere of M. domestica cv. Holsteiner Cox we observed dramatic changes in the protein composition of the apoplast of the host plant. Sequencing of the induced proteins by ESI-Q-ToF mass spectrometry and homology search identified these additional proteins as pathogenesis related proteins (PR) like ß-1,3- glucanase, thaumatin-like protein, chitinase and hevein-like protein. To confirm these findings, a suppressive subtractive hybridization with total RNA from leaves before and after inoculation of P. fluorescens Bk3 to the leaves of the host plant was performed. It revealed an increased expression level of many PR and stress related genes. The induction of PR proteins and plant defence genes in host plants after application of non-pathogenic bacterial antagonists to the plant phylloshere can presumably prevent or reduce successful infections by plant pathogens.
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Claims 5
Claim · model-written summary
Applying P. fluorescens Bk3 to the phyllosphere of apple cv. Holsteiner Cox caused dramatic changes in the apoplast protein composition of the host plant.
- Taxon
- M. domestica
Malus domesticae0 - Material
- cv. Holsteiner Coxe0
- Treatment
- application of P. fluorescens Bk3e0
- Interactor
- P. fluorescens Bk3
.JPG?width=60)
Pseudomonas fluorescense0biocontrol_agent - Outcome
- protein composition of the apoplaste0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.JPG?width=500)
Verbatim
e0“After application of P. fluorescens Bk3 to the phyllosphere of M. domestica cv. Holsteiner Cox we observed dramatic changes in the protein composition of the apoplast of the host plant”
Claim · model-written summary
The induced apoplast proteins included the pathogenesis-related proteins beta-1,3-glucanase and thaumatin-like protein.
- Taxon
- Malus domesticaMalus domesticae1
- Material
- cv. Holsteiner Coxe1
- Gene
- ß-1,3- glucanase, thaumatin-like proteine0
- Outcome
- additional proteins as pathogenesis related proteinse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“these additional proteins as pathogenesis related proteins (PR) like ß-1,3- glucanase, thaumatin-like protein”
e1“Malus domestica cv. Holsteiner Cox”
Claim · model-written summary
The induced apoplast proteins also included the pathogenesis-related proteins chitinase and hevein-like protein.
- Taxon
- Malus domesticaMalus domesticae1
- Material
- cv. Holsteiner Coxe1
- Gene
- chitinase and hevein-like proteine0
- Outcome
- additional proteins as pathogenesis related proteinse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“these additional proteins as pathogenesis related proteins (PR) like ß-1,3- glucanase, thaumatin-like protein, chitinase and hevein-like protein”
e1“Malus domestica cv. Holsteiner Cox”
Claim · model-written summary
Suppressive subtractive hybridization of leaf RNA before and after Bk3 inoculation showed increased expression of many PR and stress-related genes.
- Taxon
- Malus domesticaMalus domesticae2
- Material
- cv. Holsteiner Coxe2
- Treatment
- inoculation of P. fluorescens Bk3e0
- Interactor
- P. fluorescens Bk3Pseudomonas fluorescense0biocontrol_agent
- Gene
- PR and stress related genese1
- Outcome
- expression level of many PR and stress related genese1
- Direction
- increasede1
- Comparator
- beforee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“total RNA from leaves before and after inoculation of P. fluorescens Bk3”
e1“It revealed an increased expression level of many PR and stress related genes”
e2“Malus domestica cv. Holsteiner Cox”
Claim · model-written summary
The authors propose that induction of PR proteins and defence genes by non-pathogenic bacterial antagonists can presumably prevent or reduce successful infections by plant pathogens.
Verbatim
e0“The induction of PR proteins and plant defence genes in host plants after application of non-pathogenic bacterial antagonists to the plant phylloshere can presumably prevent or reduce successful infections by plant pathogens”
The same questions in other papers
Pseudomonas fluorescens · molecular: expression · Malus domestica (apple)Results agree
1 paper in the same direction as this paper
- positive
inoculation · molecular: expression · Malus domestica (apple)Positive and negative results
6 papers in the same direction as this paper · 4 in the opposite direction
- no effect
- negative
- no effect
7 papers more in this group
See all 11 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.