Abstract
Auxin biosynthesis was analyzed in a maize (Zea mays) kernel culture system in which the seeds develop under physiological conditions similar to the in vivo situation. This system was modified for precursor feeding experiments. Tryptophan (Trp) is efficiently incorporated into indole-3-acetic acid (IAA) with retention of the 3, 3' bond. Conversion of Trp to IAA is not competed by indole. Labeling with the general precursors [U-(13)C(6)]glucose and [1, 2-(13)C(2)]acetate followed by retrobiosynthetic analysis strongly suggest that Trp-dependent IAA synthesis is the predominant route for auxin biosynthesis in the maize kernel. The synthesis of IAA from indole glycerol phosphate and IAA formation via condensation of indole with an acetyl-coenzyme A or phosphoenolpyruvate derived metabolite can be excluded.
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Claims 5
Claim · model-written summary
Tryptophan was efficiently incorporated into IAA with retention of the 3, 3' bond.
- Compound
- Tryptophan (Trp)e0
- Outcome
- incorporated into indole-3-acetic acid (IAA) with retention of the 3, 3' bonde0
Zea maysOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Tryptophan (Trp) is efficiently incorporated into indole-3-acetic acid (IAA) with retention of the 3, 3' bond”
t0“Maize”
Claim · model-written summary
Indole did not compete with conversion of Trp to IAA.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Conversion of Trp to IAA is not competed by indole”
t0“Maize”
Claim · model-written summary
Tracer analysis strongly suggested Trp-dependent IAA synthesis predominates in maize kernels.
- Outcome
- auxin biosynthesis in the maize kernele0
- Qualifier
- Trp-dependent IAA synthesis is the predominant routee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“strongly suggest that Trp-dependent IAA synthesis is the predominant route for auxin biosynthesis in the maize kernel”
Claim · model-written summary
IAA synthesis from indole glycerol phosphate was excluded.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The synthesis of IAA from indole glycerol phosphate and IAA formation via condensation of indole with an acetyl-coenzyme A or phosphoenolpyruvate derived metabolite can be excluded”
t0“Maize”
Claim · model-written summary
IAA formation via condensation of indole with an acetyl-coenzyme A or phosphoenolpyruvate derived metabolite was excluded.
- Outcome
- IAA formation via condensation of indole with an acetyl-coenzyme A or phosphoenolpyruvate derived metabolitee0
- Qualifier
- can be excludede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“IAA formation via condensation of indole with an acetyl-coenzyme A or phosphoenolpyruvate derived metabolite can be excluded”
t0“Maize”
The same questions in other papers
indole · molecular: metabolites · Zea mays (maize)Positive and negative results
2 in the opposite direction
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.