Abstract
We report the expression of the barley (Hordeum vulgare L.) COR (cold-regulated) gene cor14b (formerly pt59) and the accumulation of its chloroplast-localized protein product. A polyclonal antibody raised against the cor14b-encoded protein detected two chloroplast COR proteins: COR14a and COR14b. N-terminal sequencing of COR14a and expression of cor14b in Arabidopsis plants showed that COR14a is not encoded by the cor14b sequence, but it shared homology with the wheat (Triticum aestivum L.) WCS19 COR protein. The expression of cor14b was strongly impaired in the barley albino mutant an, suggesting the involvement of a plastidial factor in the control of gene expression. Low-level accumulation of COR14b was induced by cold treatment in etiolated plants, although cor14b expression and protein accumulation were enhanced after a short light pulse. Light quality was a determining factor in regulating gene expression: red or blue but not far-red or green light pulses were able to promote COR14b accumulation in etiolated plants, suggesting that phytochrome and blue light photoreceptors may be involved in the control of cor14b gene expression. Maximum accumulation of COR14b was reached only when plants were grown and/or hardened under the standard photoperiod. The effect of light on the COR14b stability was demonstrated by using transgenic Arabidopsis. These plants constitutively expressed cor14b mRNAs regardless of temperature and light conditions; nevertheless, green plants accumulated about twice as much COR14b protein as etiolated plants.
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Claims 9
Claim · model-written summary
The antibody detected COR14a and COR14b in barley chloroplasts.
- Taxon
- barley
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Hordeum vulgaree0t0 - Gene
- COR14a and COR14be0
- Outcome
- two chloroplast COR proteins: COR14a and COR14be0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“A polyclonal antibody raised against the cor14b-encoded protein detected two chloroplast COR proteins: COR14a and COR14b.”
t0“The Interaction between Cold and Light Controls the Expression of the Cold-Regulated Barley Gene cor14b and the Accumulation of the Corresponding Protein1”
Claim · model-written summary
COR14a is not encoded by cor14b but shares homology with wheat WCS19.
- Taxon
- Triticum aestivum L.
Triticum aestivume0 - Gene
- WCS19 COR proteine0
- Outcome
- shared homologye0
- Comparator
- cor14b sequencee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“COR14a is not encoded by the cor14b sequence, but it shared homology with the wheat (Triticum aestivum L.) WCS19 COR protein.”
Claim · model-written summary
cor14b expression was strongly impaired in albino barley mutant an.
- Taxon
- barleyHordeum vulgaree0t0
- Material
- albino mutant ane0
- Gene
- cor14be0
- Outcome
- expression of cor14be0
- Direction
- strongly impairede0
Not reportedTreatmentComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The expression of cor14b was strongly impaired in the barley albino mutant an”
t0“The Interaction between Cold and Light Controls the Expression of the Cold-Regulated Barley Gene cor14b and the Accumulation of the Corresponding Protein1”
Claim · model-written summary
Cold induced low COR14b accumulation in etiolated plants.
- Taxon
- barleyHordeum vulgaree0t0
- Treatment
- cold treatmente0
- Gene
- COR14be0
- Outcome
- accumulation of COR14be0
- Direction
- inducede0
- Qualifier
- etiolated plantse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Low-level accumulation of COR14b was induced by cold treatment in etiolated plants”
t0“The Interaction between Cold and Light Controls the Expression of the Cold-Regulated Barley Gene cor14b and the Accumulation of the Corresponding Protein1”
Claim · model-written summary
A short light pulse enhanced cor14b expression and protein accumulation.
- Taxon
- barleyHordeum vulgaree0t0
- Treatment
- a short light pulsee0
- Gene
- cor14be0
- Outcome
- cor14b expression and protein accumulatione0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cor14b expression and protein accumulation were enhanced after a short light pulse.”
t0“The Interaction between Cold and Light Controls the Expression of the Cold-Regulated Barley Gene cor14b and the Accumulation of the Corresponding Protein1”
Claim · model-written summary
Red light promoted COR14b accumulation in etiolated plants.
- Taxon
- barleyHordeum vulgaree0t0
- Treatment
- rede0
- Gene
- COR14be0
- Outcome
- COR14b accumulatione0
- Direction
- promotee0
- Comparator
- far-red or green light pulsese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“red or blue but not far-red or green light pulses were able to promote COR14b accumulation in etiolated plants”
t0“The Interaction between Cold and Light Controls the Expression of the Cold-Regulated Barley Gene cor14b and the Accumulation of the Corresponding Protein1”
Claim · model-written summary
Blue light promoted COR14b accumulation in etiolated plants.
- Taxon
- barleyHordeum vulgaree0t0
- Treatment
- bluee0
- Gene
- COR14be0
- Outcome
- COR14b accumulatione0
- Direction
- promotee0
- Comparator
- far-red or green light pulsese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“red or blue but not far-red or green light pulses were able to promote COR14b accumulation in etiolated plants”
t0“The Interaction between Cold and Light Controls the Expression of the Cold-Regulated Barley Gene cor14b and the Accumulation of the Corresponding Protein1”
Claim · model-written summary
Standard photoperiod growth or hardening gave maximum COR14b accumulation.
- Taxon
- barleyHordeum vulgaree0t0
- Treatment
- standard photoperiode0
- Gene
- COR14be0
- Outcome
- Maximum accumulation of COR14be0
- Direction
- Maximume0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Maximum accumulation of COR14b was reached only when plants were grown and/or hardened under the standard photoperiod.”
t0“The Interaction between Cold and Light Controls the Expression of the Cold-Regulated Barley Gene cor14b and the Accumulation of the Corresponding Protein1”
Claim · model-written summary
Green transgenic Arabidopsis accumulated about twice the COR14b protein of etiolated plants.
- Taxon
- Arabidopsis
Arabidopsise0 - Treatment
- green plantse0
- Gene
- COR14be0
- Outcome
- COR14b proteine0
- Direction
- twice as muche0
- Value
- about twicee0
- Comparator
- etiolated plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The effect of light on the COR14b stability was demonstrated by using transgenic Arabidopsis. These plants constitutively expressed cor14b mRNAs regardless of temperature and light conditions; nevertheless, green plants accumulated about twice as much COR14b protein as etiolated plants.”
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See all 30 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.