Abstract
N‐ethylmaleimide (NEM) Lit 10‐100 μ M led to a strong inhibition of the auxin‐induced elongation growth of colcoptile segments, while fusicoccin‐enhanced growth was not affected. Growth inhibition occurred only if NEM and auxin were allowed to act simultaneously. Preincubation of plant segments with NEM in the absence of auxin caused no inhibition of a subsequent growth stimulation by auxin, whenever NEM was removed before the application of IAA. However, preincubation with NEM plus auxin led to a remaining growth inhibition, which could not be reversed by a second auxin incubation in the absence of NEM. Fusicoccin added to NEM‐ plus auxin‐treated segments was able to restore growth. It is suggested that auxin causes the unmasking of essential SH‐groups of a protein to which NEM links covalently. thus inhibiting the growth process. This assumption was further supported by labeling experiments wish [ 14 C]‐NEM using membranes of maize ( Zea mays L. cv. Inraplus) coleoptiles. Two membrane fractions (S 2 = 480‐1900 g; S 4 = 4300‐15000 g) revealed a significantly higher [ 14 C]‐NEM labeling in the presence of auxin (2,4‐diehlorophe‐noxyacctic acid compared to 2,6 dichlorophenoxyacetic acid). This effect disappeared when the membranes were previously washed with EGTA [ethyleneglycolbis‐(β‐aminoethylether)‐N,N,Nr′,N′‐tetraacetic acid]. The auxin‐induced sensitization of coleoptilc segments against thiol‐reagents and the auxin‐induced expression of SH‐groups of proteins of isolated membranes from coleoptiles arc suggested to be events involved in the primary action of auxins.
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Claims 9
Claim · model-written summary
NEM inhibited auxin-induced coleoptile elongation.
- Organ / stage
- colcoptile segmentse0
- Treatment
- N‐ethylmaleimide (NEM) Lit 10‐100 μ Me0
- Outcome
- auxin‐induced elongation growthe0
- Direction
- inhibitione0
Zea maysNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“N‐ethylmaleimide (NEM) Lit 10‐100 μ M led to a strong inhibition of the auxin‐induced elongation growth of colcoptile segments”
t0“maize ( Zea mays L. cv. Inraplus) coleoptiles”
Claim · model-written summary
NEM did not affect fusicoccin-enhanced growth.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“N‐ethylmaleimide (NEM) Lit 10‐100 μ M led to a strong inhibition of the auxin‐induced elongation growth of colcoptile segments, while fusicoccin‐enhanced growth was not affected.”
t0“maize ( Zea mays L. cv. Inraplus) coleoptiles”
Claim · model-written summary
Growth inhibition occurred only with simultaneous NEM and auxin exposure.
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Growth inhibition occurred only if NEM and auxin were allowed to act simultaneously.”
t0“maize ( Zea mays L. cv. Inraplus) coleoptiles”
Claim · model-written summary
NEM preincubation without auxin did not inhibit later auxin stimulation if NEM was removed.
- Treatment
- Preincubation of plant segments with NEM in the absence of auxine0
- Outcome
- subsequent growth stimulation by auxine0
- Direction
- no inhibitione0
- Qualifier
- NEM was removed before the application of IAAe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Preincubation of plant segments with NEM in the absence of auxin caused no inhibition of a subsequent growth stimulation by auxin, whenever NEM was removed before the application of IAA.”
t0“maize ( Zea mays L. cv. Inraplus) coleoptiles”
Claim · model-written summary
Preincubation with NEM and auxin left growth inhibition that a second auxin incubation did not reverse.
- Treatment
- preincubation with NEM plus auxine0
- Outcome
- remaining growth inhibitione0
- Qualifier
- could not be reversed by a second auxin incubatione0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“preincubation with NEM plus auxin led to a remaining growth inhibition, which could not be reversed by a second auxin incubation in the absence of NEM.”
t0“maize ( Zea mays L. cv. Inraplus) coleoptiles”
Claim · model-written summary
Fusicoccin restored growth after NEM and auxin treatment.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Fusicoccin added to NEM‐ plus auxin‐treated segments was able to restore growth.”
t0“maize ( Zea mays L. cv. Inraplus) coleoptiles”
Claim · model-written summary
Auxin increased NEM labeling in two maize coleoptile membrane fractions relative to the comparison compound.
- Organ / stage
- membrane fractionse0
- Treatment
- auxin (2,4‐diehlorophe‐noxyacctic acide0
- Outcome
- [ 14 C]‐NEM labelinge0
- Direction
- highere0
- Comparator
- 2,6 dichlorophenoxyacetic acide0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Two membrane fractions (S 2 = 480‐1900 g; S 4 = 4300‐15000 g) revealed a significantly higher [ 14 C]‐NEM labeling in the presence of auxin (2,4‐diehlorophe‐noxyacctic acid compared to 2,6 dichlorophenoxyacetic acid).”
t0“maize ( Zea mays L. cv. Inraplus) coleoptiles”
Claim · model-written summary
EGTA washing abolished the auxin-dependent membrane labeling effect.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This effect disappeared when the membranes were previously washed with EGTA”
t0“maize ( Zea mays L. cv. Inraplus) coleoptiles”
Claim · model-written summary
Authors suggest auxin unmasks protein SH groups that bind NEM and inhibit growth.
- Outcome
- growth processe0
- Qualifier
- unmasking of essential SH‐groups of a protein to which NEM links covalentlye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“It is suggested that auxin causes the unmasking of essential SH‐groups of a protein to which NEM links covalently. thus inhibiting the growth process.”
t0“maize ( Zea mays L. cv. Inraplus) coleoptiles”
The same questions in other papers
fusicoccin · growth · Zea mays (maize)Results differ
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- no effect
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1 paper in the same direction as this paper · 6 in the opposite direction
- positive
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See all 8 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.