Abstract
Plant programmed cell death (PCD) is a genetically controlled process that plays an important role in development and stress responses. Reactive oxygen species (ROS) are key inducers of PCD. The addition of 50 mM H₂O₂ to tobacco Bright Yellow-2 (TBY-2) cell cultures induces PCD. A comparative proteomic analysis of TBY-2 cells treated with 50 mM H₂O₂ for 30 min and 3 h was performed. The results showed early down-regulation of several elements in the cellular redox hub and inhibition of the protein repair-degradation system. The expression patterns of proteins involved in the homeostatic response, in particular those associated with metabolism, were consistently altered. The changes in abundance of several cytoskeleton proteins confirmed the active role of the cytoskeleton in PCD signalling. Cells undergoing H₂O₂-induced PCD fail to cope with oxidative stress. The antioxidant defence system and the anti-PCD signalling cascades are inhibited. This promotes a genetically programmed cell suicide pathway. Fifteen differentially expressed proteins showed an expression pattern similar to that previously observed in TBY-2 cells undergoing heat shock-induced PCD. The possibility that these proteins are part of a core complex required for PCD induction is discussed.
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Claims 9
Claim · model-written summary
Hydrogen peroxide induced programmed cell death in tobacco Bright Yellow-2 cultures.
- Taxon
- tobacco
Nicotiana tabacume0 - Material
- Bright Yellow-2 (TBY-2)e0
- Treatment
- 50 mM H₂O₂e0
- Outcome
- PCDe0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The addition of 50 mM H₂O₂ to tobacco Bright Yellow-2 (TBY-2) cell cultures induces PCD.”
Claim · model-written summary
Hydrogen peroxide treatment caused early down-regulation of several redox hub elements.
- Taxon
- tobaccoNicotiana tabacumt0
- Material
- Bright Yellow-2t0
- Treatment
- 50 mM H₂O₂e1
- Outcome
- several elements in the cellular redox hube0
- Direction
- down-regulatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The results showed early down-regulation of several elements in the cellular redox hub”
e1“TBY-2 cells treated with 50 mM H₂O₂ for 30 min and 3 h”
t0“tobacco Bright Yellow-2 cells”
Claim · model-written summary
Hydrogen peroxide treatment inhibited the protein repair-degradation system.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- 50 mM H₂O₂e1
- Outcome
- protein repair-degradation systeme0
- Direction
- inhibitione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“inhibition of the protein repair-degradation system”
e1“TBY-2 cells treated with 50 mM H₂O₂ for 30 min and 3 h”
t0“tobacco Bright Yellow-2 cells”
Claim · model-written summary
Homeostatic response protein expression patterns, particularly metabolism-related proteins, were altered.
- Taxon
- tobaccoNicotiana tabacumt0
- Outcome
- expression patterns of proteins involved in the homeostatic responsee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The expression patterns of proteins involved in the homeostatic response, in particular those associated with metabolism, were consistently altered.”
t0“tobacco Bright Yellow-2 cells”
Claim · model-written summary
Cytoskeleton protein abundance changed during programmed cell death.
- Taxon
- tobaccoNicotiana tabacumt0
- Outcome
- abundance of several cytoskeleton proteinse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The changes in abundance of several cytoskeleton proteins confirmed the active role of the cytoskeleton in PCD signalling.”
t0“tobacco Bright Yellow-2 cells”
Claim · model-written summary
Tobacco cells undergoing peroxide-induced programmed cell death failed to cope with oxidative stress.
- Taxon
- tobaccoNicotiana tabacumt0
- Outcome
- fail to cope with oxidative stresse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cells undergoing H₂O₂-induced PCD fail to cope with oxidative stress.”
t0“tobacco Bright Yellow-2 cells”
Claim · model-written summary
The antioxidant defence system was inhibited during peroxide-induced programmed cell death.
- Taxon
- tobaccoNicotiana tabacumt0
- Outcome
- antioxidant defence systeme0
- Direction
- inhibitede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The antioxidant defence system and the anti-PCD signalling cascades are inhibited.”
t0“tobacco Bright Yellow-2 cells”
Claim · model-written summary
Anti-PCD signalling cascades were inhibited during peroxide-induced programmed cell death.
- Taxon
- tobaccoNicotiana tabacumt0
- Outcome
- anti-PCD signalling cascadese0
- Direction
- inhibitede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The antioxidant defence system and the anti-PCD signalling cascades are inhibited.”
t0“tobacco Bright Yellow-2 cells”
Claim · model-written summary
Fifteen proteins had differential expression resembling the pattern previously seen in heat shock induced cell death.
- Taxon
- tobaccoNicotiana tabacumt0
- Outcome
- differentially expressed proteinse0
- Value
- Fifteene0
- Comparator
- TBY-2 cells undergoing heat shock-induced PCDe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Fifteen differentially expressed proteins showed an expression pattern similar to that previously observed in TBY-2 cells undergoing heat shock-induced PCD.”
t0“tobacco Bright Yellow-2 cells”
The same questions in other papers
hydrogen peroxide · cytology · Nicotiana tabacum (tobacco)Positive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- positive
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.