Abstract
Active oxygen species (AOS), especially hydrogen peroxide, play a critical role in the defence of plants against invading pathogens and in the hypersensitive response (HR). This is characterized by the induction of a massive production of AOS and the rapid appearance of necrotic lesions is considered as a programmed cell death (PCD) process during which a limited number of cells die at the site of infection. This work was aimed at investigating the mode of cell death observed in cultures of BY‐2 tobacco cells exposed to H 2 O 2 . It was shown that H 2 O 2 is able to induce various morphological cell death features in cultured tobacco BY‐2 cells. The hallmarks of cell death observed with fluorescent and electron microscopy differed greatly with the amount of H 2 O 2 added to the cell culture. The appearance of nuclear fragmentation similar to ‘apoptotic bodies’ associated with a fragmentation of the nuclear DNA into small fragments appear for almost 18% of the cells treated with 12.5 mM H 2 O 2 . The early stages of the induction of this PCD process consisted in cell shrinkage and chromatin condensation at the periphery of the nucleus. Above 50 mM, H 2 O 2 induces high necrotic cell death. These data suggest that H 2 O 2 ‐induced cell damage is associated with the induction of various cell death processes that could be involved differently in plant defence reactions.
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Claims 5
Claim · model-written summary
Hydrogen peroxide induced multiple morphological cell death features.
- Taxon
- tobacco
Nicotiana tabacume0 - Treatment
- H 2 O 2e0
- Outcome
- morphological cell death featurese0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“H 2 O 2 is able to induce various morphological cell death features in cultured tobacco BY‐2 cells”
Claim · model-written summary
Cell death features varied greatly with hydrogen peroxide amount.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- amount of H 2 O 2 addede0
- Outcome
- hallmarks of cell deathe0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“hallmarks of cell death observed with fluorescent and electron microscopy differed greatly with the amount of H 2 O 2 added to the cell culture”
t0“tobacco”
Claim · model-written summary
At 12.5 mM hydrogen peroxide nearly 18% of cells showed nuclear fragmentation and DNA fragmentation.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- 12.5 mM H 2 O 2e0
- Outcome
- nuclear fragmentation similar to ‘apoptotic bodies’ associated with a fragmentation of the nuclear DNA into small fragmentse0
- Value
- almost 18%e0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“nuclear fragmentation similar to ‘apoptotic bodies’ associated with a fragmentation of the nuclear DNA into small fragments appear for almost 18% of the cells treated with 12.5 mM H 2 O 2”
t0“tobacco”
Claim · model-written summary
Early programmed cell death showed cell shrinkage and peripheral chromatin condensation.
- Taxon
- tobaccoNicotiana tabacumt0
- Outcome
- cell shrinkage and chromatin condensation at the periphery of the nucleuse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“early stages of the induction of this PCD process consisted in cell shrinkage and chromatin condensation at the periphery of the nucleus”
t0“tobacco”
Claim · model-written summary
Above 50 mM hydrogen peroxide induced high necrotic cell death.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- Above 50 mM, H 2 O 2e0
- Outcome
- necrotic cell deathe0
- Direction
- highe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Above 50 mM, H 2 O 2 induces high necrotic cell death”
t0“tobacco”
The same questions in other papers
hydrogen peroxide · cytology · Nicotiana tabacum (tobacco)Positive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- positive
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.