Paper · 2015 · Journal of Shaanxi Normal University

The effects of the cyanide-resistant respiration on the cell viability and hydrogen peroxide production of the tobacco suspension cells under ZnCl_2 stress

Feng Hanqin

10
claims
24
verbatim quotes
0
citations
1
research areas
—
Not open access

Abstract

By using the tobacco(Nicotiana tabacum L.cv.Bright Yellow)cell culture,the effects of the cyanide-resistant respiration on the cell viability and hydrogen peroxide production under ZnCl2 stress were studied.The results showed that with the increase of the concentrations of ZnCl2,the level of cell viability is decreased,while the level of the cyanide-resistant respiration is increased.A higher level of ZnCl2(150 mmol/L)stress caused a significant decrease of cell viability and a significant increase of the hydrogen peroxide(H2O2)production.Treatment with exogenous H2O2 also caused a decrease of cell viability,and application of the scavenger of H2O2 alleviated the decrease of cell viability under ZnCl2 stress.The effects of the cyanide-resistant respiration on the cell viability and hydrogen peroxide production under ZnCl2 stress were further studied.The ZnCl2-stressed(150mmol/L)cells pretreated with salicylhydroxamic acid(an inhibitor of the cyanide-resistant respiration)had lower cell viability but higher H2O2 production than the cells exposed to150mmol/L ZnCl2 stress only.These results suggest that the cyanide-resistant respiration can function in alleviating the decrease of cell viability and oxidative stress under ZnCl2 stress and thus could be involved inthe tolerance of plants to ZnCl2 stress.

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The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 10

Associationobservedoriginal resultnegativemeasured

Claim · model-written summary

With rising ZnCl2 concentration, tobacco cell viability decreased.

Material
Bright Yellowe1
Treatment
the concentrations of ZnCl2e2
Outcome
cell viabilitye2
Direction
decreasede2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Nicotiana tabacum
Nicotiana tabacum · TaxonJom / Joachim Müllerchen · CC BY 2.5 · Wikimedia Commons ↗

Verbatim

e1

“tobacco(Nicotiana tabacum L.cv.Bright Yellow)cell culture”

✓ located verbatim in the source · abstract, characters 13–70 · show in text ↑
e2

“with the increase of the concentrations of ZnCl2,the level of cell viability is decreased”

✓ located verbatim in the source · abstract, characters 231–320 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

With rising ZnCl2 concentration, the cyanide-resistant respiration of tobacco cells increased.

Material
Bright Yellowe1
Treatment
the concentrations of ZnCl2e2
Outcome
cyanide-resistant respiratione3
Direction
increasede3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Nicotiana tabacum
Nicotiana tabacum · TaxonJom / Joachim Müllerchen · CC BY 2.5 · Wikimedia Commons ↗

Verbatim

e1

“tobacco(Nicotiana tabacum L.cv.Bright Yellow)cell culture”

✓ located verbatim in the source · abstract, characters 13–70 · show in text ↑
e2

“with the increase of the concentrations of ZnCl2,the level of cell viability is decreased”

✓ located verbatim in the source · abstract, characters 231–320 · show in text ↑
e3

“while the level of the cyanide-resistant respiration is increased”

✓ located verbatim in the source · abstract, characters 321–386 · show in text ↑
Intervention effectobservedoriginal resultnegativesignificant (as stated)measured

Claim · model-written summary

ZnCl2 at 150 mmol/L significantly decreased tobacco cell viability.

Material
Bright Yellowe1
Treatment
ZnCl2(150 mmol/L)stresse4
Outcome
cell viabilitye4
Direction
decreasee4

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Nicotiana tabacum
Nicotiana tabacum · TaxonJom / Joachim Müllerchen · CC BY 2.5 · Wikimedia Commons ↗

Verbatim

e1

“tobacco(Nicotiana tabacum L.cv.Bright Yellow)cell culture”

✓ located verbatim in the source · abstract, characters 13–70 · show in text ↑
e4

“A higher level of ZnCl2(150 mmol/L)stress caused a significant decrease of cell viability”

✓ located verbatim in the source · abstract, characters 387–476 · show in text ↑
Intervention effectobservedoriginal resultnegativesignificant (as stated)measured

Claim · model-written summary

ZnCl2 at 150 mmol/L significantly increased H2O2 production in tobacco cells.

Material
Bright Yellowe1
Treatment
ZnCl2(150 mmol/L)stresse4
Outcome
hydrogen peroxide(H2O2)productione5
Direction
increasee5

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Nicotiana tabacum
Nicotiana tabacum · TaxonJom / Joachim Müllerchen · CC BY 2.5 · Wikimedia Commons ↗

Verbatim

e1

“tobacco(Nicotiana tabacum L.cv.Bright Yellow)cell culture”

✓ located verbatim in the source · abstract, characters 13–70 · show in text ↑
e4

“A higher level of ZnCl2(150 mmol/L)stress caused a significant decrease of cell viability”

✓ located verbatim in the source · abstract, characters 387–476 · show in text ↑
e5

“a significant increase of the hydrogen peroxide(H2O2)production”

✓ located verbatim in the source · abstract, characters 481–544 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Exogenous H2O2 decreased tobacco cell viability.

Material
Bright Yellowe1
Treatment
Treatment with exogenous H2O2e6
Outcome
cell viabilitye6
Direction
decreasee6

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Nicotiana tabacum
Nicotiana tabacum · TaxonJom / Joachim Müllerchen · CC BY 2.5 · Wikimedia Commons ↗

Verbatim

e1

“tobacco(Nicotiana tabacum L.cv.Bright Yellow)cell culture”

✓ located verbatim in the source · abstract, characters 13–70 · show in text ↑
e6

“Treatment with exogenous H2O2 also caused a decrease of cell viability”

✓ located verbatim in the source · abstract, characters 545–615 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

A scavenger of H2O2 alleviated the decrease of tobacco cell viability under ZnCl2 stress.

Material
Bright Yellowe1
Treatment
application of the scavenger of H2O2e7
Outcome
decrease of cell viabilitye7
Direction
alleviatede7

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Nicotiana tabacum
Nicotiana tabacum · TaxonJom / Joachim Müllerchen · CC BY 2.5 · Wikimedia Commons ↗

Verbatim

e1

“tobacco(Nicotiana tabacum L.cv.Bright Yellow)cell culture”

✓ located verbatim in the source · abstract, characters 13–70 · show in text ↑
e7

“application of the scavenger of H2O2 alleviated the decrease of cell viability under ZnCl2 stress”

✓ located verbatim in the source · abstract, characters 620–717 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Pretreatment with salicylhydroxamic acid lowered cell viability of ZnCl2-stressed tobacco cells compared with ZnCl2 stress alone.

Material
Bright Yellowe1
Treatment
pretreated with salicylhydroxamic acide0
Outcome
cell viabilitye0
Direction
lowere0
Comparator
150mmol/L ZnCl2 stress onlye2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Nicotiana tabacum
Nicotiana tabacum · TaxonJom / Joachim Müllerchen · CC BY 2.5 · Wikimedia Commons ↗

Verbatim

e1

“tobacco(Nicotiana tabacum L.cv.Bright Yellow)cell culture”

✓ located verbatim in the source · abstract, characters 13–70 · show in text ↑
e0

“pretreated with salicylhydroxamic acid(an inhibitor of the cyanide-resistant respiration)had lower cell viability”

✓ located verbatim in the source · abstract, characters 897–1010 · show in text ↑
e2

“higher H2O2 production than the cells exposed to150mmol/L ZnCl2 stress only”

✓ located verbatim in the source · abstract, characters 1015–1090 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Pretreatment with salicylhydroxamic acid raised H2O2 production of ZnCl2-stressed tobacco cells compared with ZnCl2 stress alone.

Material
Bright Yellowe1
Treatment
pretreated with salicylhydroxamic acide0
Outcome
H2O2 productione2
Direction
highere2
Comparator
150mmol/L ZnCl2 stress onlye2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Nicotiana tabacum
Nicotiana tabacum · TaxonJom / Joachim Müllerchen · CC BY 2.5 · Wikimedia Commons ↗

Verbatim

e1

“tobacco(Nicotiana tabacum L.cv.Bright Yellow)cell culture”

✓ located verbatim in the source · abstract, characters 13–70 · show in text ↑
e0

“pretreated with salicylhydroxamic acid(an inhibitor of the cyanide-resistant respiration)had lower cell viability”

✓ located verbatim in the source · abstract, characters 897–1010 · show in text ↑
e2

“higher H2O2 production than the cells exposed to150mmol/L ZnCl2 stress only”

✓ located verbatim in the source · abstract, characters 1015–1090 · show in text ↑
Mechanismauthor interpretationoriginal resultpositivebasis n/a

Claim · model-written summary

Cyanide-resistant respiration can function in alleviating the decrease of cell viability and oxidative stress under ZnCl2 stress.

Material
Bright Yellowe1
Outcome
the decrease of cell viability and oxidative stresse0
Qualifier
cyanide-resistant respiration can function in alleviatinge0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Nicotiana tabacum
Nicotiana tabacum · TaxonJom / Joachim Müllerchen · CC BY 2.5 · Wikimedia Commons ↗

Verbatim

e1

“tobacco(Nicotiana tabacum L.cv.Bright Yellow)cell culture”

✓ located verbatim in the source · abstract, characters 13–70 · show in text ↑
e0

“the cyanide-resistant respiration can function in alleviating the decrease of cell viability and oxidative stress under ZnCl2 stress”

✓ located verbatim in the source · abstract, characters 1118–1250 · show in text ↑
Mechanismhypothesisoriginal resultpositivebasis n/a

Claim · model-written summary

Cyanide-resistant respiration could be involved in the tolerance of tobacco to ZnCl2 stress.

Material
Bright Yellowe1
Outcome
tolerance of plants to ZnCl2 stresse0
Qualifier
could be involvede0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Nicotiana tabacum
Nicotiana tabacum · TaxonJom / Joachim Müllerchen · CC BY 2.5 · Wikimedia Commons ↗

Verbatim

e1

“tobacco(Nicotiana tabacum L.cv.Bright Yellow)cell culture”

✓ located verbatim in the source · abstract, characters 13–70 · show in text ↑
e0

“could be involved inthe tolerance of plants to ZnCl2 stress”

✓ located verbatim in the source · abstract, characters 1260–1319 · show in text ↑

hydrogen peroxide · cytology · Nicotiana tabacum (tobacco)Positive and negative results

3 papers: 1 positive, 2 negative or no effect

2 in the opposite direction

  1. negative
  2. negative
See all 3 papers →

salicylhydroxamic acid · physiology: oxidative · Nicotiana tabacum (tobacco)Results agree

2 papers: 0 positive, 2 negative or no effect

1 paper in the same direction as this paper

  1. negative
See all 2 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.