Abstract
Cucumber (Cucumis sativus) hypocotyls were cut to one-half of their diameter transversely, and morphological and histochemical analyses of the process of tissue reunion in the cortex were performed. Cell division in the cortex commenced 3 d after cutting, and the cortex was nearly fully united within 7 d. 4',6-Diamidino-2-phenylindole staining and 5-bromo-2'-deoxyuridine labeling experiments indicate that nDNA synthesis occurred during this process. In addition, specific accumulation of pectic substances was observed in the cell wall of attached cells in the reunion region of the cortex. Cell division during tissue reunion was strongly inhibited when the cotyledon was removed. This inhibition was reversed by applying gibberellin (GA, 10(-4) M GA3) to the apical tip of the cotyledon-less plant. Supporting this observation, cell division in the cortex was inhibited by treatment of the cotyledon with 10(-4) M uniconazole-P (an inhibitor of GA biosynthesis), and this inhibition was also reversed by simultaneous application of GA. In contrast to the essential role of cotyledon, normal tissue reunion in cut hypocotyls was still observed when the shoot apex was removed. The requirement of GA for tissue reunion in cut hypocotyls was also evident in the GA-deficient gib-1 mutant of tomato (Lycopersicon esculentum). Our results suggest that GA, possibly produced in cotyledons, is essential for cell division in reuniting cortex of cut hypocotyls.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by-nc-nd. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 11
Claim · model-written summary
Cucumber cortex cell division began three days after cutting.
- Taxon
- Cucumber
Cucumis sativust0 - Organ / stage
- cortexe0
- Outcome
- Cell division in the cortexe0
- Value
- 3 de0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cell division in the cortex commenced 3 d after cutting”
t0“Cucumber and Tomato Hypocotyls”
Claim · model-written summary
The cut cucumber cortex was nearly united within seven days.
- Taxon
- CucumberCucumis sativust0
- Organ / stage
- cortexe0
- Outcome
- cortex was nearly fully unitede0
- Value
- 7 de0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the cortex was nearly fully united within 7 d.”
t0“Cucumber and Tomato Hypocotyls”
Claim · model-written summary
Nuclear DNA synthesis occurred during cucumber tissue reunion.
- Taxon
- CucumberCucumis sativust0
- Outcome
- nDNA synthesis occurrede0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“4',6-Diamidino-2-phenylindole staining and 5-bromo-2'-deoxyuridine labeling experiments indicate that nDNA synthesis occurred during this process.”
t0“Cucumber and Tomato Hypocotyls”
Claim · model-written summary
Pectic substances specifically accumulated in cell walls of attached cells in the cortex reunion region.
- Taxon
- CucumberCucumis sativust0
- Organ / stage
- cell wall of attached cellse0
- Outcome
- accumulation of pectic substancese0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“specific accumulation of pectic substances was observed in the cell wall of attached cells in the reunion region of the cortex.”
t0“Cucumber and Tomato Hypocotyls”
Claim · model-written summary
Removing the cotyledon strongly inhibited cell division during tissue reunion.
- Taxon
- CucumberCucumis sativust0
- Treatment
- cotyledon was removede0
- Outcome
- Cell division during tissue reunione0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cell division during tissue reunion was strongly inhibited when the cotyledon was removed.”
t0“Cucumber and Tomato Hypocotyls”
Claim · model-written summary
Applying GA3 reversed inhibition in cotyledon-less plants.
- Taxon
- CucumberCucumis sativust0
- Treatment
- applying gibberellin (GA, 10(-4) M GA3)e0
- Outcome
- inhibitione0
- Direction
- reversede0
- Qualifier
- cotyledon-less plante0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This inhibition was reversed by applying gibberellin (GA, 10(-4) M GA3) to the apical tip of the cotyledon-less plant.”
t0“Cucumber and Tomato Hypocotyls”
Claim · model-written summary
Cotyledon treatment with uniconazole-P inhibited cortex cell division.
- Taxon
- CucumberCucumis sativust0
- Treatment
- treatment of the cotyledon with 10(-4) M uniconazole-Pe0
- Outcome
- cell division in the cortexe0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cell division in the cortex was inhibited by treatment of the cotyledon with 10(-4) M uniconazole-P (an inhibitor of GA biosynthesis)”
t0“Cucumber and Tomato Hypocotyls”
Claim · model-written summary
Simultaneous GA application reversed the uniconazole-P inhibition of cortex cell division.
- Taxon
- CucumberCucumis sativust0
- Treatment
- simultaneous application of GAe0
- Outcome
- inhibitione0
- Direction
- reversede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“this inhibition was also reversed by simultaneous application of GA.”
t0“Cucumber and Tomato Hypocotyls”
Claim · model-written summary
Removing the shoot apex still allowed normal reunion of cut hypocotyl tissue.
- Taxon
- CucumberCucumis sativust0
- Treatment
- shoot apex was removede0
- Outcome
- normal tissue reunione0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“normal tissue reunion in cut hypocotyls was still observed when the shoot apex was removed.”
t0“Cucumber and Tomato Hypocotyls”
Claim · model-written summary
The GA-deficient gib-1 tomato mutant showed that GA is required for cut-hypocotyl tissue reunion.
- Taxon
- tomato (Lycopersicon esculentum)
Solanum lycopersicume0 - Material
- gib-1 mutante0
- Outcome
- requirement of GA for tissue reunione0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The requirement of GA for tissue reunion in cut hypocotyls was also evident in the GA-deficient gib-1 mutant of tomato (Lycopersicon esculentum).”
Claim · model-written summary
The authors infer that GA, possibly produced in cotyledons, is essential for cell division in the reuniting cortex.
- Taxon
- CucumberCucumis sativust0
- Compound
- GAe0
- Outcome
- cell division in reuniting cortexe0
- Qualifier
- possibly produced in cotyledonse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GA, possibly produced in cotyledons, is essential for cell division in reuniting cortex of cut hypocotyls.”
t0“Cucumber and Tomato Hypocotyls”
The same questions in other papers
Gibberellic acid · disease · Cucumis sativus (cucumber)Results agree
1 paper in the same direction as this paper
- positive
class · disease · Cucumis sativus (cucumber)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.